Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
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Target Concepts:
Gene/Protein
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Query: UNIPROT:P15088 (
mast cell
)
14,925
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Several fibroblast growth factors (FGFs) are able to reduce and improve radiation-induced tissue damage through the activation of surface fibroblast growth factor receptors (FGFRs). In contrast, some FGFs lack classical signal sequences, which play roles in the release of FGFs, and the intracellular function of these FGFs is not well clarified. In this study, we evaluated the transcript levels of 22 FGFs in a human
mast cell
line, HMC-1, using quantitative RT-PCR and found that FGF2 and
FGF12
were expressed in HMC-1 cells.
FGF12
not only lacks classical signal sequences but also fails to activate FGFRs. HMC-1 cells were transfected with an expression vector of
FGF12
to clarify the intracellular function of
FGF12
after irradiation. The overexpression of
FGF12
in HMC-1 cells decreased ionizing radiation-induced apoptosis, and siRNA-mediated repression of
FGF12
expression augmented apoptosis in HMC-1 cells. The overexpression of
FGF12
strongly suppressed the marked augmentation of apoptosis induced by inhibition of the MEK/ERK pathway with PD98059. In contrast, the mitogen-activated protein kinase (MAPK) scaffold protein islet brain 2 (IB2), which was reported to bind to
FGF12
, did not interfere with the anti-apoptotic effect of
FGF12
. The expression of
FGF12
transcripts was also detected in murine cultured mast cells derived from bone marrow or fetal skin. These findings suggest that
FGF12
intracellularly suppresses radiation-induced apoptosis in mast cells independently of IB2.
...
PMID:Involvement of intracellular expression of FGF12 in radiation-induced apoptosis in mast cells. 1852 61
