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Query: UNIPROT:P15088 (mast cell)
 14,925 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mast cells (MCs) are important effector cells in mucosal defense and allergic inflammation. Lymphoid tissues such as tonsils and adenoids also have modest numbers of MCs. However, the role of MCs in lymphoid tissues is not well known. In this study, we showed the local distribution of MCs in tonsils from IgE-mediated allergic or nonallergic donors, studied their ultrastructure, and specified their surrounding cell types. Tonsils were obtained from IgE-mediated allergic or nonallergic donors suffering from chronic tonsillitis or hyperplastic tonsils. The localization and distribution of MCs and IgE binding to MCs were determined by immunohistochemistry using antibodies against tryptase, c-kit, and IgE. In addition, mast cell structure was examined using electron microscopy. In both allergic and nonallergic donors, MCs were distributed mainly in the interfollicular area and the perivascular area of connective tissue. We found that the total number of MCs in tonsils was almost the same in both donor groups. However, the number of MCs in the interfollicular area was significantly higher in nonallergic versus allergic donors. Moreover, the electron microscopy revealed that MCs localized in perivascular areas of connective tissue have scroll-type granules, and MCs in the interfollicular areas contained both particle and scroll-type granules. In addition, the MCs that were surrounded by CD4+ lymphocytes in the interfollicular area showed empty granules, whereas MCs in the perivascular area, not surround by CD4+, were intact. This implies that these MCs were degranulated, and this probably was caused by CD4+ cells. Taken together, these findings suggest that tonsillar MCs distribute differently in allergic and nonallergic donors, and that MCs in the interfollicular area might be activated by direct contact with CD4+ T cells.
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PMID:Comparison of human tonsillar mast cell localization and ultrastructural observations between IgE-mediated allergic and nonallergic donors. 1706 73

Mast cell take active part in immune system of body, clinical manifestations are closely related to hypersensitivity. Reactions and potent biological mediators released by these cells are responsible for different clinical manifestation like allergic reaction. Frequent and important sites of these allergic reactions occur in-upper aero digestive tract, nasal, pharyngeal and respiratory allergies. The study aims to demonstrate different types of heparin in mast cells having role in tonsillitis. Present work is to study distribution and histochemical nature of mast cells in human palatine tonsil in patients suffering from chronic tonsillitis. 20 cases were studied during the study. Diagnosis was done on clinical criteria-Anterior pillar flushing. Expulsion of cheesy material from the tonsillar crypts on pressing with tongue depressor. Palpable jugulodigastric group of lymph nodes. Dissected Tonsillar material fixed into fixative-staining done. For qualitative histological observation Harris Haemotoxylene with eosin used as counter stain. Normal Histological study of mast cell like size, shape, granules were done. Distribution of mast cells in different parts of Tonsillar Tissue was observed. Histochemical study of mast cells to determine nature of reactive substance was done. Tonsil contains mucous glands, no granular tissue in present study of chronic inflammatory reactions feasible in form of fibrosis, lymphatic infiltration in epithelium. Morphology of mast cells with toluidine blue at pH 2 and 4.4 gave better picture. Mast cells had varied shape and size in different places and points of same field of focus, oval, elongated, fusiform, pleomorphic in shape. Nucleus is central ill-defined masked by granules. Cytoplasm contains mast cell granules that stained blue with Alcian blue stain, purple to bluish purple with toluidine blue. Distribution of mast cells in present study: Mast cells were found at all places mostly concentrated at capsules. Lamina propria-1-2/hpf, capsule-4-5/hpf, connective tissue septa-1-2/hpf. Histochemical Feature- In present study, histochemical study remained confined to connective tissue septa of mast cells and biochemical nature of Heparin contained in it. In present work, the identification of mast cells is based on metachromatic reactions of its granules with acidified toluidine blue at pH 2 and buffered toluidine blue at pH 4.4 to identify mast cell granules containing higher and lower sulphates of heparin.
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PMID:Histological and Histochemical Observations on Mast Cell and Glands in Chronic Tonsillitis. 3174 25