Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P15088 (mast cell)
 14,925 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Continuous mouse bone marrow cultures were infected with Friend murine leukemia virus. Production of nonadherent (NA) and adherent cells, granulocyte-macrophage colony-forming unit(s) of progenitor cells (GM-CFUc), pluripotential hematopoietic stem cells (CFUs), the self-renewal potential (Rs) of CFUs, and generation of factor-dependent (FD) multipotential and committed permanent stem cell cloned lines were measured. Uninfected marrow cultures from C57BL/6J, C57BL/6JUt, B6.S, C3H/HeJ, (C57BL/6J x DBA/2J)F1, CD- 1 Swiss, or N:NIH(S) mice generated NA cells, GM-CFUc, and CFUs for 20-41 weeks; cultures infected with Rauscher or other helper viruses generated them for 35-45 weeks. GM-CFUc and CFUs production in SFFV-positive cultures persisted for over 65 weeks and exceeded control levels by twentyfold to fiftyfold. The Rs of CFUs in SFFV-positive cultures was not detectably increased above control cultures. Multipotential (erythroid-neutrophil-mast cell-basophil-eosinophil) permanent FD cell clones were derived from control and SFFV-positive cultures. Thus SFFV amplifies the stem cell pool in vitro without detectably increasing the Rs capacity of CFUs.
J Natl Cancer Inst 1983 Feb
PMID:Pool size of pluripotential hematopoietic stem cells increased in continuous bone marrow culture by Friend spleen focus-forming virus. 657 39

Most early-phase testing of new therapeutic modalities involves analysis of initial tumor response as opposed to estimation of long-term response. In this study, the validity of initial response rates to predict long-term responses was examined for tumors treated with radiotherapy alone compared with heat combined with radiotherapy. A total of 130 pet animals with either squamous cell carcinomas, melanomas, fibrosarcomas, mammary adenocarcinomas, or mast cell sarcomas were randomized to receive either radiation alone (XRT) or heat + radiation (delta + XRT). Responses to treatment were evaluated by response rates and response duration. The complete response (CR) rates were consistently higher for delta + XRT than for XRT across different histology groups. The combined therapy led to prolonged tumor response in all histological subgroups except melanomas, which had a longer response duration when treated with XRT alone (p = 0.043). This was in spite of a relatively high CR rate in that group (100% versus 12.5% for delta + XRT and XRT, respectively). In contrast, while no significant improvement in CR rate was observed for dermal squamous cell carcinomas treated with delta + XRT (XRT = 52.9%; delta + XRT = 68.8%), a significant improvement in response duration was noted (p = 0.002). These are two examples where CR rate did not predict long-term response. When all histological subgroups were combined (except melanomas), the CR rate was higher (p less than 0.001), and response duration was prolonged (p = 0.031) for delta + XRT compared to XRT alone.
Cancer Res 1983 Dec
PMID:Correlation between initial and long-term responses of spontaneous pet animal tumors to heat and radiation or radiation alone. 664 May 25

The accumulation of mast cells is characteristic of a number of pathological states. We demonstrate here the directional motility of mast cells in vitro in response to tumor-derived peptides. Rat peritoneal mast cells were isolated on Percoll gradients and maintained in serum-free medium containing transferrin, albumin, soybean lipid, and cholesterol. The isolated mast cells migrated under agarose in response to medium conditioned by any of eight tumor cell lines but not to medium conditioned by any of a variety of nontumorigenic cell types. The tumor-derived activity is dialyzable (cutoff, Mr 3500), stable to trypsin treatment and to heating at 56 degrees, but destroyed by heating to 100 degrees or by treatment with Streptomyces griseus protease or carboxypeptidase A. Ultrafiltration suggests a molecular weight of 300 to 1000. Two tripeptides, glycylhistidyllysine and N-formylmethionylleucylphenylalanine, were also found to be potent chemoattractants for mast cells. N-Formylmethionylphenylalanine and valylglycylserylglutamic acid (eosinophil chemotactic Factor A) had significantly less chemoattractant activity over the same range of concentrations. Several peptide analogues of glycylhistidyllysine were tested and found to have no activity. The growth of capillary blood vessels toward a growing tumor is generally preceded by an accumulation of mast cells at the tumor site. Based on the results presented here and previous data from our laboratory on mast cell stimulation of capillary endothelial cell migration, we propose an hypothesis that the chemoattraction of mast cells by tumor-derived peptides may be an important early event in tumor neovascularization.
Cancer Res 1983 Dec
PMID:Stimulation of rat peritoneal mast cell migration by tumor-derived peptides. 664 May 37

A total of 130 dogs and cats with squamous cell carcinomas, melanomas, fibrosarcomas, mammary adenocarcinomas, or mast cell sarcomas were randomized to receive radiation (XRT) or heat plus XRT. Time-temperature data for each monitored tumor location were converted to degree-minutes or equivalent min at 43 degrees (Eq43). Response rates and durations of response were compared for subgroups of histology, volume, site, and heat treatment method. Thermal gradients existed in all heated tumors. The influence of these gradients on tumor response was examined by correlation of response with degree-minutes and Eq43 minima, maxima, averages, and ranges. A pattern emerged from these analyses linking dose minima, maxima, and ranges with prognostic subgroups as classified by volume, site, or treatment method. The data indicated that the coolest part of the tumor governed the biological response to combined heat + XRT. Tumors which received a minimum of 35 Eq43 had significantly longer durations of response than did those receiving XRT alone or less than 3 Eq43 (p less than or equal to 0.006 and 0.014, respectively; log-rank test). Furthermore, broad temperature ranges were associated with power-limiting "hot spots" and invariably led to underheating in other areas of tumor. Multivariate analysis found minimum Eq43 on the first treatment to be the best predictor of long-term response (p less than 0.05). Other biological covariates of site, volume, and histology contributed strength to the model, which was independent of Eq43 (p less than 0.05).
Cancer Res 1984 Jan
PMID:Importance of minimum tumor temperature in determining early and long-term responses of spontaneous canine and feline tumors to heat and radiation. 669 58

Mast cell colonies grew in methylcellulose when mouse spleen cells were cultured for 14 days in the presence of medium conditioned by concanavalin A-stimulated spleen cells. Tumor-promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate, enhanced the concanavalin A-stimulated spleen cell-conditioned medium-induced colony growth of mast cell progenitors. The effect of TPA on this colony growth was blocked by the addition of two antipromoters of experimental skin carcinogenesis, i.e., retinoic acid and dexamethasone. These results show that TPA has the ability to enhance the clonal proliferation of mouse mast cells and that this effect of TPA is closely related to tumor-promoting activity.
Cancer Res 1984 May
PMID:Enhancement of clonal proliferation of mouse mast cells by a tumor-promoting phorbol ester. 671 2

Thymoma was diagnosed in 15 dogs at Angell Memorial Animal Hospital between 1972 and 1983. All thymomas developed in the cranial portion of the mediastinum. An autoimmune paraneoplastic syndrome was observed in 10 (67%) of the dogs and included myasthenia gravis, nonthymic neoplasms, and polymyositis. Clinical signs were variable and inconsistent, depending on whether they were attributable to the cranial mediastinal mass or to the paraneoplastic syndrome. Eleven dogs were necropsied. Two thymomas had gross characteristics of malignancy. In 3 cases, a cell consistent with a subclass of mast cell was found and in 1 thymoma, melanocytes were observed.
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PMID:Clinical and pathologic features of thymoma in 15 dogs. 673 56

The effects of the specific active cancer immunotherapy utilizing autologous tumor tissue particles polymerized with ethylchlorformiate on the immune system of the host were evaluated in DBA/2 mice bearing a malignant mast cell tumor, mastocytoma (P-815 X 2), with the special emphasis placed on the morphologic changes in the lymph nodes. In assessing the lymph node morphology in relation to the immunologically reactive lymphocyte populations (T- and B-cells), the standardized reporting system was employed, and the post-capillary venule score (PCV-S), shown to be related to the T-cell activity, was calculated for each lymph node. The specific cancer immunotherapy instituted along with the PPD tuberculin as adjuvant was capable of reverting to a considerable degree the profound depletion of the T-cell population in the paracortex of the lymph nodes, as well as exerting a stimulatory influence on the B-cells responsible for antibody synthesis in the cortex and medulla of the nodes. The results were interpreted to favor the view that the favorable influence on the tumor rejection previously shown to be exerted by the specific immunotherapy technique studied, most probably is attributable to the observed stimulatory effects of it on the lymphocyte populations involved in cell-mediated and humoral immune responses. The appropriate cooperation of both T- and B-lymphocytes is most probably needed to ensure the most effective host response against the tumor cells in this system.
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PMID:Immunological reactivity in the lymph nodes of the host following experimental cancer immunotherapy utilizing polymerized autologous tumor tissue particles. 677 10

Ultrastructural and ultracytochemical studies were performed on blood and bone marrow specimens from 18 patients with Philadelphia chromosome-positive blastic leukemia; 7 patients were in blast transformation following a typical history of chronic myelogenous leukemia and 11 patients presented with "acute leukemia." The patients were divided into 2 morphologic groups on the basis of light microscopic and cytochemical observations. In group I, which consisted of 11 patients, the proliferating cells were "lymphoid" in appearance and demonstrated many cytochemical, biochemical, and immunologic features similar to those of the lymphoblasts of non-T, non-B acute lymphoblastic leukemia. In group II, which consisted of 7 patients, the proliferating cells were myeloid in appearance. On the basis of ultrastructural observations, the 11 group I patients were divided into 2 subgroups, A and B. Subgroup IA, consisting of 5 patients, was characterized by blasts that demonstrated no differentiating features. In subgroup IB, consisting of 6 patients, 20-30% of the leukemic cells contained inclusions that resembled leukemic mast cell or basophil granules. The leukemic cells in the 7 group II patients manifested myeloid characteristics by light microscopy and prominent basophil and mast cell granulopoiesis by electron microscopy. Abnormalities of other myeloid cell lines were also observed in both the lymphoid and myeloid groups of patients.
J Natl Cancer Inst 1980 Sep
PMID:Ultrastructural features of basophil and mast cell granulopoiesis in blastic phase Philadelphia chromosome-positive leukemia. 693 33

Mast cell-deficient W/Wv mice had an increased tumor incidence after subcutaneous treatment with 3-methylcholanthrene, compared with that in normal congenic mice treated in the same way. This increased tumor incidence was suppressed to the normal level when the carcinogen was given after the mast cell deficiency had been overcome by transplantation of bone marrow cells from normal congenic mice. The W/Wv mice, however, were not defective in natural killer and T-cell-mediated cytotoxic activities. These results support the hypothesis that mast cells are involved in tumor suppression.
J Natl Cancer Inst 1982 Dec
PMID:Evidence for involvement of mast cells in tumor suppression in mice. 698 95

A xenoantiserum raised against a mast cell tumor line (FMP1.1) was found to have cross-reactivity with surface antigens on primitive hemopoietic precursor cells in normal mouse bone marrow: erythroid burst-forming units; granulocyte-macrophage colony-forming cells; and high-proliferative-potential granulocyte-macrophage colony-forming cells. In the presence of anti-FMP1.1 serum and complement, only 15 +/- 2% (S.E.) (n = 5) of normal nucleated marrow cells were lysed, demonstrating that the majority of mature hemopoietic cells did not express the antigens detected on their primitive counterparts. A variety of hemopoietic and other tumor cell lines were examined with anti-FMP1.1 serum, and all B- and pre-B-lymphomas, one plasmacytoma, one mastocytoma, and a monocyte-macrophage line exhibited significant lysis. Direct typing of the FMP1.1 tumor demonstrated that it did not express the B-cell surface antigens such as Ia, surface immunoglobulin, Fc, and complement (C3) receptors. Although the Ly.6 alloantigen was present on FMP1.1 cells, this antigen was not found on marrow erythroid burst-forming units and granulocyte-macrophage colony-forming units. Absorption of anti-FMP1.1 serum with cross-reacting (WEHI-3 and W-279.1) and a nonreacting (P-815) cell line confirmed the specificity of the antiserum reaction with these cells and marrow progenitors. These experiments indicated that more than one antibody is contained in anti-FMP1.1 serum. Thus, the mast cell tumor (FMP1.1) carries an unusual array of antigens, which are found on bone marrow progenitors and are not expressed on the majority of differentiated cells. It has been demonstrated that tumor cell lines provide an important basis for the analysis of surface membrane antigens expressed on normal hemopoietic progenitors.
Cancer Res 1981 Oct
PMID:Surface membrane antigens of hemopoietic tumor cell lines and normal marrow progenitor cells. 728 8


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