Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P14784 (
IL-2 receptor
)
3,849
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Mechanism of action of
Deoxyspergualin
(
DSG
) in vitro has not been clarified, because it is unstable in solution. I studied its mechanism in vitro using Deoxymethylspergulin (MeDSG) that is stable in solution. Action of MeDSG was studied using human lymphocyte and a human T cell clone which was established in our laboratory. MeDSG showed dose dependent inhibition of blastogenesis in MLR. MeDSG inhibited the response by about 50% at the concentration of 10 micrograms/ml. It is a very interesting point that MeDSG added at 3 days later suppressed MLR. But MeDSG did not suppress MLR added at 4 days later or 2nd MLR. MeDSG did not have effects on the production of IL-1, IL-2 and the expression of
IL-2 receptor
on stimulated lymphocyte by mitogen. But MeDSG suppressed IFN-gamma production induced stimulation of alloantigen at only concentration of 1 microgram/ml. MeDSG did not suppress proliferation of helper T cell clone. On the other hand, CsA suppressed
IL-2 receptor
expression and proliferation of helper T cell clone. These results showed MeDSG act on relatively late phase of immunoreaction. From in vitro results, when we use
DSG
for clinical acute rejection, it is more appropriate to use
DSG
and Methylprednisolone at the same time to get early recovery.
...
PMID:[Study of immunosuppressive mechanism of deoxymethylspergualin in vitro]. 835 45
