Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P14784 (IL-2 receptor)
 3,849 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A double staining technique for simultaneously determinating cell surface phenotype and the degree of cell activation is described. As an activation marker, the tetrazolium dye MTT has been used. Cells were incubated for 30 min with MTT. Activated cells yielded a granular staining pattern. Upon termination of the reaction with sodium azide, a double-step immunofluorescence staining procedure using monoclonal antibodies specific for cell surface antigens was performed. The percentage of cells simultaneously displaying MTT formation and fluorescence was microscopically evaluated. Our results demonstrate that MTT staining is expressed concomitantly with the IL-2 receptor and the transferrin receptor. This method permits a simple characterization of activated T cell subsets and can be used clinically to analyse the T cell functions of patients being treated with immunosuppressive agents and patients with acquired immune deficiency syndrome.
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PMID:Detection of activated lymphocyte subsets by fluorescence and MTT staining. 246 13

By using ELISA and assay of MTT participating in IL-6 dependent cell clone. The authors measured the circulating levels of soluble IL-2 receptor (sIL-2R) and IL-6 in patients undergoing hemodialysis (HD). Alterations of the above-mentioned parameters before and after a three-month course of treatment with Chinese drug Epimedium sagittatum on the same HD patients. It is confirmed that in patients with end-stage renal failure (ESRF), sIL-2R level elevated significantly, while IL-6 level decreased apparently (P < 0.01). Furthermore, levels of both sIL-2R and IL-6 could be restored to normal after treatment with Epimedium sagittatum. These findings indicated not only the presence of immunodeficiency in ESRF, but also the effectiveness of regulation with Chinese drug Epimedium sagittatum.
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PMID:[Effect of epimedium sagittatum on soluble IL-2 receptor and IL-6 levels in patients undergoing hemodialysis]. 779 53

The proliferative response of CTLL-2 cells to human recombinant interleukin-2 (IL-2) can be modeled mathematically using enzyme kinetic equations. This approach has been used to analyze dose-response curves (IL-2 concentration vs. level of proliferation) measured by MTT and [3H]TdR assays. The values of functional dissociation constants, equivalent to IL-2 concentrations giving 50% of the maximal response, depended on the cell concentration and increased from 4 to 60 pM for the [3H]TdR assay and from 40 to 140 pM for the MTT assay when the cell concentration was increased from 2 x 10(3) to 4 x 10(4) cells/well. The types of inhibition and dissociation constants for various inhibitors of IL-2-dependent proliferation such as mAbs against IL-2 receptor (7D4 and AMT13) and normal mouse serum (NMS) were also analyzed. Both mAbs exhibited competitive mechanisms of inhibition whereas NMS inhibited IL-2-driven proliferation in a mixed manner. Two gel-filtration fractions of NMS with inhibitory activity manifested different types of inhibition: purely competitive type of inhibition in the case of a 10-15 kDa fraction and a mixed type of inhibition for a 100-150 kDa fraction. The proposed model can also be used for quantitative analysis of the influence of various factors (pH, temperature, cultivation condition) on the level of proliferation.
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PMID:Quantitative analysis of interleukin-2-induced proliferation in the presence of inhibitors using a mathematical model. 844 49

In this paper, we determined the number of peripheral blood lymphocytes (PBL) in the elderly and their proliferative response to PHA (Phytohemagglutinin). The data showed that the PBL number decreased with age. For the young group: 1680.0 +/- 644.7/microliters; the 60-69 years old group: 1284.0 +/- 492.3/microliters; the 70-79 years old group: 987.5 +/- 309.1/microliters, P < 0.05. The OD (optical density) value measured by MTT method for proliferative response of PBL in the elderly was 0.15 +/- 0.08, which was lower than the young group 0.18 +/- 0.05. Also, an apparent decline of soluble IL-2 receptor (sIL-2R) release from activated PBL in old people was found. The results suggested that the changes of PBL number and sIL-2R level could be considered as an indication of immune function of lymphocytes in the elderly.
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PMID:The monitoring biomarker for immune function of lymphocytes in the elderly. 914 69

Immunologic effector cells termed cytokine-induced killer (CIK) cells are generated in vitro from peripheral blood lymphocytes by addition of interferon-gamma, interleukin (IL)-2, IL-1 and an antibody against CD3. CIK cells have been shown to eradicate established tumors in a SCID mouse/human lymphoma model. CIK cells are dependent on exogenous cytokines such as IL-2, IL-7, or IL-12. We studied the effect of these cytokines in detail. Cellular proliferation was analyzed using an MTT proliferation assay, surface antigen expression via flow cytometry, cytotoxic activity using an LDH release assay, and apoptosis via flow cytometric analysis. IL-2, IL-7 and IL-12 led to significant growth of lymphocytes. Cells grown in IL-2 and IL-7 showed higher proliferation rates than cells grown in IL-12 according to the MTT assay. Concerning surface antigen expression, exogenous IL-7 led to a decrease in IL-7 receptor expression (4.8% from 60.4%) and exogenous IL-2 to a decrease in IL-2 receptor expression (61.2% from 73.2%). CD28 expression was higher in cells grown in IL-7 (77.3%) than in cells grown in IL-2 (62.5%). IL-12 led to a decrease in ICAM-1 adhesion molecule expression (57.7% from 76.7%) and an increase in CD56 expression compared with exogenous IL-7. IL-7 led to higher number of CD4-positive cells than IL-2 (53.0% vs 49.5%). No significant difference was found between IL-2, IL-7 and IL-12 in cytotoxic activity measured in an LDH release assay. Small amounts of apoptotic cells were found with all cytokines. However, the percentage of necrotic cells was higher with exogenous IL-12 than with IL-2 or IL-7. In summary, CIK cells can be generated using exogenous IL-2, IL-7 or IL-12. No difference in cytotoxic activity was found. However, significant differences were found in cell proliferation rates, antigen expression and percentage of necrotic cells.
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PMID:Generation of cytokine-induced killer cells using exogenous interleukin-2, -7 or -12. 987 75