Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P14784 (IL-2 receptor)
 3,849 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Long-term S-antigen (S-Ag) specific T lymphocyte lines can induce experimental autoimmune uveoretinitis (EAU) when transferred into naive rats systemically or intravitreally. The uveitogenic lymphocyte line (ThS) stains positively for the ART 18 (IL-2 receptor) and W3/25 (T helper/inducer); negatively for OX6 (RT1B) and OX8 (T suppressor/cytotoxic cells). The inflammation induced by systemic or intravitreal transfer of the ThS line was studied with respect to the surface markers of the infiltrating cells, as well as the markers expressed by the resident ocular cells, by immunohistopathological techniques. On day 4 to 5 after systemic injection of the ThS line, rare T cells (W3/25+, ART 18+) and macrophages (OX42+, OX6+) surrounded by some resident cells that expressed MHC class II antigens were identified in the ciliary body and choroid. Shortly thereafter (10-20 hr) more macrophages, polymorphonuclear leukocytes, and T lymphocytes (W3/25+, then OX8+) appeared in the inflamed eye. The kinetics was similar to EAU induced by active immunization. The rats with severe disease expressed the MHC class II antigens on large numbers of resident cells in the eye. Intravitreally transferred ThS cells migrated to the retina within 24 hr of transfer. Infiltration of macrophages (OX6+, OX42+) and other T lymphocytes (OX6+, W3/25+ or OX8+), in conjunction with photoreceptor damage, were observed within the next 24-48 hr. The cells with the ThS markers disappeared from the eye on day 4-5 post transfer. These findings suggest that the ThS line can recognize the photoreceptor S-Ag in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Dynamics of experimental autoimmune uveoretinitis induced by adoptive transfer of S-antigen-specific T cell line. 325 50

T-cell lines, initially expanded with either mitogens or the uveitogenic S-antigen obtained from the eyes of three uveitis patients, were maintained in vitro for 4-8 weeks. S-antigen specific T-cell clones were derived from the peripheral blood of a fourth patient. The surface marker characteristics clearly demonstrate these cells to be T-cells. Both OKT4+ and OKT8+ clones and cell lines were found using immunofluorescence microscopy, an ELISA technique, or the laser cytofluorograph, but some could be only definitely identified as T-cells with the antibody to the IL-2 receptor, and not the OKT series of monoclonal anti-sera. These studies underscore how dynamic the expression of T-cell membrane markers can be, and that commercially available monoclonal anti-sera to T-cell membrane markers may not identify all T-cells in long term culture. This is the first report of the isolation of an antigen specific T-cell clone relevant to the eye, and the technique described will allow clonal expansion of ocular cells in order to study their functional characteristics.
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PMID:Long-term antigen specific and non-specific T-cell lines and clones in uveitis. 636 32