Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P11684 (Uteroglobin)
 114 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Uteroglobin(utg) is a potent phospholipase A2 inhibitor but is genetically distinct from lipocortins. The purpose of the present investigation was to biochemically and immunologically characterize the utg-like antigen from rabbit plasma and serum that were found to be highly positive by radioimmunoassay(RIA). The RIA standard curve of pure rabbit utg from the uterus is compared with utg-like protein in circulation, and the curves are parallel to each other. Concerning the western blot of utg-like protein as compared with utg standard, it is clear that there is a distinct protein band corresponding to the two monomers of utg(7 kDa). Moreover, the rise in endometrial utg synthesis that occurs upon progesterone(P) treatment in rabbits is paralleled by a dramatic decrease in endometrial PGE2, PGF2 alpha levels. The level of utg-like protein in circulation increased the level of this protein approximately three-fold in the serum (70 ng/ml without Pvs 216 ng/ml with P), whereas dexamethasone(Dex) increased it two-fold. To determine the source of this protein in circulation, we cannulated the uterine and the pulmonary veins of rabbits primed with different steroids. The levels of utg-like protein in the uterine venous plasma versus peripheral venous plasma were as follows: 379 ng/ml vs 216 ng/ml, treated P. The pulmonary venous plasma was compared with the peripheral venous samples (1240 ng/ml vs 127 ng/ml, treated Dex). The results of the present study indicate that utg-like protein is detectable in the circulation of the rabbit.
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PMID:[Uteroglobin: a phospholipase A2 inhibitory protein in rabbit blood]. 279 60

Uteroglobin (UG) gene encodes a cytokine-like, multifunctional, antiinflammatory protein, with potent phospholipase A2-inhibitory activity. It has been suggested that during implantation this protein protects the embryos from maternal immunological assault, facilitates the maintenance of quiescence in the uterus throughout pregnancy, prevents the onset of premature labor, and helps maintain an inflammation-free respiratory organ. This latter function of UG is suggested to be accomplished by preventing hydrolysis of surfactant phospholipids by a lung-specific phospholipase A2. Using reverse transcription polymerase chain reaction, in situ hybridization, immunofluorescence, and radioimmunoassay, we studied UG gene expression in the rabbit uterus throughout gestation and in the fetal lung. Here, we report that: (a) contrary to previous reports, UG gene expression in the rabbit uterus occurs throughout gestation with a precipitous decline just before parturition; (b) this gene expression is dramatically increased in the fetal lung with increasing gestational age; and (c) while there is an inverse relationship between the levels of UG, PGE2, and PGF2 alpha, a positive correlation was found in that of UG and leukotriene C4 in the fetal lung. Our results raise the possibility that dysregulation of UG gene expression, at least in part, may contribute to the onset of premature labor and the development of inflammatory lung disease in premature neonates.
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PMID:Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung. Relationship between uteroglobin and eicosanoid levels in the developing fetal lung. 761 4

Uteroglobin (UG, Clara cell secretory protein) is a steroid inducible, multifunctional protein that is secreted by the mucosal epithelia. UG has anti-proliferative and anti-metastatic effects in cancer cells. COX-2, which catalyzes the first step in the synthesis of prostanoids, has been shown to be overexpressed in tumors. This study investigated the effect of UG on the inhibition of COX-2 expression in lung cancer cells. The level of the COX-2 protein and its mRNA were decreased by UG, as demonstrated by Western blot and the RT-PCR, respectively. The EIA shows that UG suppressed PGE2 synthesis. Western blot showed that the NF-kappaB nuclear translocation was inhibited by the transduction of UG. In addition, an EMSA demonstrated the inhibition of the NF-kappaB-DNA binding by UG. The luciferase assay showed that UG also inhibited the NF-kappaB-mediated transcription activity. Furthermore, transfection of the lung cancer cell lines with the COX-2 reporter gene constructs demonstrated that the transcription of COX-2 gene was suppressed by UG. These results show that the inhibition of COX-2 expression by UG transduction correlated with the suppression of NF-kappaB activity in the lung cancer cells. This suggests that UG have the possibility for the treatment of lung cancer.
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PMID:Adenovirus-uteroglobin suppresses COX-2 expression via inhibition of NF-kappaB activity in lung cancer cells. 1582 19