Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P11021 (
BiP
)
2,049
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The phenotypes of
calbindin-D9k
- (
CaBP-9k
-) knockout (KO),
calbindin
-
D28k
- (
CaBP-28k
-) KO, and
CaBP-9k/28k
-KO mice are similar to those of wild-type (WT) mice due to the compensatory action of other calcium transport proteins. In this study, we investigated the expression of cellular prion protein (PrP
C
) in the brains of
CaBP-9k
-,
CaBP-28k
-, and
CaBP-9k/28k
-KO mice. PrP
C
expression was significantly upregulated in the brain of all three strains. Levels of phospho-Akt (Ser473) and phospho-Bad (Ser136) were significantly elevated, but those of phospho-ERK and phospho-Bad (Ser155 and 112) were significantly reduced in the brains of
CaBP-9k
-,
CaBP-28k
-, and
CaBP-9k/28k
-KO mice. The expressions of the Bcl-2, p53, Bax, Cu/Zn-SOD, and Mn-SOD proteins were decreased in the brains of all KO mice. Expression of the endoplasmic reticulum marker protein
BiP
/GRP78 was decreased, and that of the CHOP protein was increased in the brains of those KO mice. To identify the roles of
CaBP-28k
, we transfected PC12 cells with siRNA for
CaBP-28k
and found increased expression of the PrP
C
protein compared to the levels in control cells. These results suggest that
CaBP-28k
expression may regulate PrP
C
protein expression and these mice may be vulnerable to the influence of prion disease.
...
PMID:
Calbindin-D28k
in the Brain Influences the Expression of Cellular Prion Protein. 2954 46
