Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P11021 (BiP)
 2,049 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Micro/nano-topography (MNT) promotes osteogenic differentiation of stem cells, but the topographical signaling transduction mechanisms remain unclear. We have confirmed that MNT as a stressor triggers endoplasmic reticulum (ER) stress and activates unfolded protein response (UPR) in rBMMSCs, and such topography-induced ER stress improves osteogenic differentiation. In order to reveal the influence of nanotube dimension on ER stress, MNTs containing vertically oriented TiO2 nanotubes with defined diameters between 30 and 100 nm were fabricated on pure titanium (Ti) foils, then ER stress and osteogenic differentiation of cells was systematically investigated. After cultivating for 12 h, cells on MNTs showed gross distortion of rough ER morphology that containing the electron-dense material through the transmission electron microscopy (TEM) examination, and the expansion of the ER lumen became more pronounced as the dimension of nanotubes increased. Additionally, PCR and western blotting showed that the ER stress-related gene the ER chaperone 78 kDa glucose-regulated protein or immunoglobulin binding protein (GRP78/BiP) was up-regulated dependent on the nanotube dimension, which was in line with the osteogenic induction capabilities of MNTs. Our findings further revealed the mechanism for topographical cues modulating osteogenic differentiation of cells, which may provide an innovated criterion of the optimal design of implant surface topography.
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PMID:Micro/nano topography with altered nanotube diameter differentially trigger endoplasmic reticulum stress to mediate bone mesenchymal stem cell osteogenic differentiation. 3303 6