Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10721 (
c-kit
)
6,575
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Our recent study showed that bradykinin increases cell cycling progression and migration of human cardiac
c-Kit
+
progenitor cells by activating pAkt and pERK1/2 signals. This study investigated whether bradykinin-mediated Ca
2+
signalling participates in regulating cellular functions in cultured human cardiac
c-Kit
+
progenitor cells using laser scanning confocal microscopy and biochemical approaches. It was found that bradykinin increased cytosolic free Ca
2+
(
Ca
i
2
+
) by triggering a transient Ca
2+
release from ER IP3Rs followed by sustained Ca
2+
influx through store-operated Ca
2+
entry (SOCE) channel. Blockade of B2 receptor with HOE140 or IP3Rs with araguspongin B or silencing
IP3R3
with siRNA abolished both Ca
2+
release and Ca
2+
influx. It is interesting to note that the bradykinin-induced cell cycle progression and migration were not observed in cells with siRNA-silenced
IP3R3
or the SOCE component TRPC1, Orai1 or STIM1. Also the bradykinin-induced increase in pAkt and pERK1/2 as well as cyclin D1 was reduced in these cells. These results demonstrate for the first time that bradykinin-mediated increase in free
Ca
i
2
+
via ER-
IP3R3
Ca
2+
release followed by Ca
2+
influx through SOCE channel plays a crucial role in regulating cell growth and migration via activating pAkt, pERK1/2 and cyclin D1 in human cardiac
c-Kit
+
progenitor cells.
...
PMID:Bradykinin-mediated Ca
2+
signalling regulates cell growth and mobility in human cardiac c-Kit
+
progenitor cells. 3011 80
