Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P10415 (Bcl-2)
 33,771 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A new member of the Bcl-2 family was identified, Bcl-G. The human BCL-G gene consists of 6 exons, resides on chromosome 12p12, and encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) and Bcl-G(S) have identical sequences for the first 226 amino acids but diverge thereafter. Among the Bcl-2 homology (BH) domains previously recognized in Bcl-2 family proteins, the BH3 domain is found in both Bcl-G(L) and Bcl-G(S), but only the longer Bcl-G(L) protein possesses a BH2 domain. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L). Apoptosis induction by Bcl-G(S) depended on the BH3 domain and was suppressed by coexpression of anti-apoptotic Bcl-X(L) protein. Bcl-X(L) also coimmunoprecipitated with Bcl-G(S) but not with mutants of Bcl-G(S) in which the BH3 domain was deleted or mutated or with Bcl-G(L). Bcl-G(S) was predominantly localized to cytosolic organelles, whereas Bcl-G(L) was diffusely distributed throughout the cytosol. A mutant of Bcl-G(L) in which the BH2 domain was deleted displayed increased apoptotic activity and coimmunoprecipitated with Bcl-X(L), suggesting that the BH2 domain autorepresses Bcl-G(L).
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PMID:Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. 1105 13

BCL-G, also known as Bcl2-like14, is a unique member of the Bcl-2 family that plays an important role in regulating apoptosis in humans. In the present study, we assessed the biological activities of porcine BCL-G (pBCL-G). The open reading frame (ORF) of pBCL-G covered 990 bp and encoded 329 amino acids. The genomic structure of the pBCL-G gene was also determined. The deduced amino acid sequence of the pBCL-G cDNA was highly identical to homologs in other species. Furthermore, domain prediction showed that pBCL-G protein contains BH2 and BH3 domains, which are typical domains of the Bcl-2 family. Phylogenetic analysis indicated that BCL-G may function differently among species. Subcellular localization analysis showed that GFP-pBCL-G fusion protein is distributed in the nucleus and cytoplasm. Flow cytometric analysis proved that pBCL-G is a pro-apoptotic factor. This study is useful for understanding pBCL-G and offers a potential molecular model for the investigation of diseases related to human BCL-G.
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PMID:BCL-G as a new candidate gene for immune responses in pigs: bioinformatic analysis and functional characterization. 2298 May 16

BCL-G, an apoptotic factor in Bcl-2 family, is involved in several kinds of diseases by interacting with several proteins. Although many studies on mouse and human BCL-G have been reported, porcine BCL-G (pBCL-G) has been little investigated. In this study, our results showed that pBCL-G was universally expressed in porcine tissues. The BH2 domain affected the subcellular distribution of pBCL-G protein. pBCL-G could interact with porcine JAB1 (pJAB1), by which its subcellular distribution was affected. pBCL-G promoted staurosporine-induced apoptosis that was significantly enhanced by interaction of pBCL-G with pJAB1. The apoptosis at least partially depended on the activated caspase-8, -9 and -3. Owing to the close phylogenetic distance between pigs and humans and their many physiological similarities, our findings may provide a potential molecular model to study human BCL-G and also may have implications in the treatment of diseases relevant with BCL-G.
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PMID:A potential molecular model for studying apoptosis enhanced by the interaction of BCL-G with JAB1 in swine. 2754 39