Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10415 (
Bcl-2
)
33,771
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
ZNF689
, a C2H2-type of zinc finger transcription factor, was suggested to play a key role in hepatocarcinogenesis. However, none of the target genes or potential roles of
ZNF689
in hepatocellular carcinoma (HCC) have been elucidated. Here, we investigated the role of
ZNF689
in HCC cell lines focusing on cell viability and apoptosis.We found that the knockdown of
ZNF689
by its specific siRNA decreased cell viability of Huh7. Cell cycle analysis revealed that the
ZNF689
knockdown increased the proportion of the sub-G1 population, accompanied by an increase of annexin V- and TUNEL-positive cells.Western blot analysis revealed that
ZNF689
knockdown induced the expression of pro-apoptotic factors of
Bcl-2
family, Bax, Bak and jBid. There was a correlation between the expression of
ZNF689
and an anticancer drug 5-fluorouracil (5-FU) resistance of HCC cells. In vivo,
ZNF689
siRNA reduced tumor viability in HepG2-bearing mice with statistical significance. Furthermore, immunohistochemical analysis demonstrated that nuclei of a significant portion of human HCC surgical specimens were positive for
ZNF689
. Taken together, our results indicate that
ZNF689
blocks pro-apoptotic signaling by suppressing the Bak/Bax/Bid pathway, resulting in the progression of liver cancer and resistance to 5-FU.
ZNF689
may be a promising chemotherapeutic target against liver cancer.
...
PMID:ZNF689 suppresses apoptosis of hepatocellular carcinoma cells through the down-regulation of Bcl-2 family members. 2162 62
