Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10415 (
Bcl-2
)
33,771
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Neuroblastoma breakpoint family member 1
(
NBPF1
) is involved in the occurrence and development of tumors. However, only a limited number of studies were conducted on
NBPF1
and cutaneous squamous cell carcinoma (SCC). This study mainly explored the expression and mechanism of
NBPF1
in SCC. SCC tissue and adjacent tissues samples were randomly selected.
NBPF1
gene was overexpressed in the A431 cell line using plasmid transfection technique. Cell viability was tested by cell counting kit-8 (CCK-8) assay. Flow cytometry was used to determine cell cycle and apoptosis. Western blot and RT-qPCR were respectively performed to determine the expression levels of proteins and mRNAs. The
NBPF1
gene was lowly expressed in SCC tissues. The expression level of
NBPF1
gene was the lowest in A431 cell line. The cell viability of A431 was reduced after transfection. Overexpression of
NBPF1
not only arrested A431 cells in G1 phase and promoted apoptosis, but also up-regulated the expressions of Bax and p53 mRNA and protein and down-regulated the expressions of
Bcl-2
, Survivin and Cyclin D1. Akt-p53-Cyclin pathway was inhibited when
NBPF1
gene expression was up-regulated. Upregulation of
NBPF1
might promote apoptosis of A431 cells and block cell cycle via inhibiting the activation of Akt-p53-Cyclin signaling pathway.
...
PMID:Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma cells. 3105 34
