Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10415 (
Bcl-2
)
33,771
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Objective To observe the expression of the
neural precursor cell expressed, developmentally down-regulated 9
(
NEDD9
) in esophageal cancer, to investigate the impact of decreased expression of
NEDD9
on invasion and migration, and to explicit the function of
NEDD9
in EC109 human esophageal cancer cell line. Methods Immunohistochemical staining was used to detect the expression of
NEDD9
in human esophageal cancer tissues and paracancerous normal tissues. RNA interfering (RNAi) was used to knockdown
NEDD9
in EC109 cells. The interference efficiency was detected by reverse transcription PCR (RT-PCR) and Western blot analysis. Cell proliferation was determined by MTT assay and the invasion and migration abilities of EC109 cells were monitored by Transwell
TM
assay. The protein levels of proliferating cell nuclear antigen (PCNA), Bax and
Bcl-2
were tested by Western blotting. Results The positive expression rate of
NEDD9
in esophageal carcinoma tissues was significantly higher compared with that in the paracancerous tissues. After
NEDD9
expression was successfully downregulated in EC109 cells by siRNA, the proliferation, invasion and migration rates in transfection group were significantly lower than those in control group; meanwhile, the expression of
Bcl-2
was reduced and Bax expression was enhanced. Conclusion The protein expression level of
NEDD9
is higher in esophageal carcinoma tissues than that in adjacent normal tissues. Knockdown of
NEDD9
expression can restrain the proliferation, invasion and migration of EC109 cells.
...
PMID:[Knockdown of NEDD9 inhibits the proliferation, invasion and migration of esophageal carcinoma EC109 cells]. 2791 98
