Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10415 (
Bcl-2
)
33,771
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Scopoletin is a promising acaricidal botanical natural compound against
Tetranychus cinnabarinus
, and its acaricidal mechanism maybe involve calcium overload according to our previous study. To seek potential candidate target genes of calcium overload induced by scopoletin in
T. cinnabarinus
, RNA-seq was utilized to detect changes in transcription levels. 24 and 48 h after treatment, 70 and 102 differentially expressed genes were obtained, respectively. Target genes included 3 signal transduction genes, 4 cell apoptosis genes, 4 energy metabolism genes, and 2 transcription factor genes. The role of 3 calcium signaling pathway-related genes, namely, G-protein-coupled neuropeptide receptor,
Bcl-2
protein and
guanylate kinase
(designated
TcGPCR
,
TcBAG
, and
TcGUK
, respectively) in the calcium overload were investigated in this study. RT-qPCR detection showed that scopoletin treatment upregulated the expression level of
TcGPCR
and downregulated the expression level of
TcBAG
and
TcGUK
. The result of RNAi indicated that downregulation of
TcGPCR
decreased susceptibility to scopoletin, and downregulation of
TcBAG
and
TcGUK
enhanced susceptibility to scopoletin. Functional expression in Chinese hamster ovary cells showed that scopoletin induced a significant increase in intracellular free calcium [Ca
2+
]i levels by activating
TcGPCR.
These results demonstrated that the acaricidal mechanism of scopoletin was via disrupting intracellular Ca
2+
homeostasis and calcium signaling pathway mediated by GPCR, BAG, and GUK.
...
PMID:Acaricidal Mechanism of Scopoletin Against
Tetranychus cinnabarinus
. 3089 18
