Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10415 (
Bcl-2
)
33,771
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Gami-soyosan is a medicinal herbal formulation prescribed for the treatment of menopausal symptoms, including
hot flashes
and osteoporosis. Gami-soyosan is also used to treat similar symptoms experienced by patients with breast cancer. The incidence of breast cancer in women receiving hormone replacement therapy is a big burden. However, little is known about the components and their mechanism of action that exhibit these beneficial effects of Gami-soyosan. The aim of this study was to simultaneously analyze compounds of Gami-soyosan, and determine their cytotoxic effects on estrogen receptor (ER)-positive MCF-7 human breast adenocarcinoma cells. We established a simultaneous analysis method of 18 compounds contained in Gami-soyosan and found that, among the various compounds in Gami-soyosan, gallic acid (
1
), decursin (
17
), and decursinol angelate (
18
) suppressed the viability of MCF-7 cells. Gallic acid (
1
), decursin (
17
), and decursinol angelate (
18
) induced apoptotic cell death and significantly increased poly (ADP-ribose) polymerase (PARP) cleavage and the
Bcl-2
-associated X protein/ B-cell lymphoma 2 (Bax/
Bcl-2
) ratio. Decursin (
17
) increased the expression of cleaved caspases-8, -9, -7, and -3. Decursinol angelate (
18
) increased the expression of cleaved caspase-8 and -7. These three components altered the different apoptosis signal pathways. Collectively, gallic acid (
1
), decursin (
17
), and decursinol angelate (
18
) may be used to inhibit cell proliferation synergistically in patients with ER-positive breast cancer.
...
PMID:Analysis and Identification of Active Compounds from Gami-Soyosan Toxic to MCF-7 Human Breast Adenocarcinoma Cells. 3129 70
