Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10415 (
Bcl-2
)
33,771
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
To investigate the expression of
Bcl-2
and Bcl-xl gene in sensitive (A2780) and drug-resistance (
AD6
) human ovarian cancer cell lines and explore the molecular mechanism of multidrug resistance, A2780 and
AD6
were detected by using DNA gel electrophoresis, flow cytometry and RT-PCR. Our results showed that (1) "DNA ladder" was observed in A2780 and
AD6
after cisplatin treatment; (2) after 3.0, 6.0, 9.9 microg/ml of cisplatin treatment, a significant difference was noted in the rate of apoptosis between in A2780 and
AD6
(P<0.05); (3)
Bcl-2
and Bcl-xl genes were overexpressed in
AD6
. After cisplatin treatment, the expression of
Bcl-2
and Bcl-xl genes was down-regulated in A2780 and
AD6
. It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells, and the over-expression of
Bcl-2
and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance of human ovarian cancer.
...
PMID:Difference in expression of Bcl-2 and Bcl-xl genes in cisplatin-sensitive and cisplatin-resistant human in ovarian cancer cell lines. 1531 67
