Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P10145 (
IL-8
)
23,849
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The pneumonic lesions and mortality caused by Mannheimia haemolytica in bighorn sheep (
BHS
; Ovis canadensis) are more severe than those in the related species, domestic sheep (DS; Ovis aries), under both natural and experimental conditions. Leukotoxin (Lkt) and lipopolysaccharide (LPS) are the most important virulence factors of this organism. One hallmark of pathogenesis of pneumonia is the influx of polymorphonuclear leukocytes (PMNs) into the lungs. Lkt-induced cytolysis of PMNs results in the release of cytotoxic compounds capable of damaging lung tissue.
Interleukin-8
(
IL-8
) is a potent PMN chemoattractant. The objective of the present study was to determine if there is differential expression of
IL-8
by the macrophages and PMNs of
BHS
and DS in response to M. haemolytica. Macrophages and PMNs of
BHS
and DS were stimulated with heat-killed M. haemolytica or LPS.
IL-8
expression by the cells was measured by enzyme-linked immunosorbent assays and real-time reverse transcription-PCR (RT-PCR). The PMNs of
BHS
expressed severalfold higher levels of
IL-8
than those of DS upon stimulation. Lesional lung tissue of M. haemolytica-infected
BHS
contained significantly higher levels of
IL-8
than nonlesional tissue. The bronchoalveolar lavage (BAL) fluid of infected
BHS
also contained higher levels of
IL-8
than that of infected DS. Depletion of
IL-8
reduced migration of PMNs toward BAL fluid by approximately 50%, indicating that
IL-8
is integral to PMN recruitment to the lung during M. haemolytica infection. Excessive production of
IL-8
, enhanced recruitment of PMNs, and PMN lysis by Lkt are likely responsible for the severity of the lung lesions in M. haemolytica-infected
BHS
.
...
PMID:Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infection. 2051 32
