Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P08908 (5-HT1A)
 5,574 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monocytes, recovered directly from peripheral blood by counter-current centrifugal elutriation (CCE), were shown to provide two regulatory signals for induction of interferon-gamma (IFN-gamma) in natural killer (NK) cells in response to interleukin-2 (IL-2): an upregulating signal and an inhibitory signal. The inhibitory signal was time-dependent, irreversible, and operating on a pretranslational level, as indicated by the inability of enriched NK cells to accumulate IFN-gamma mRNA in the presence of elutriated monocytes. Monocyte-induced inhibition of IFN-gamma production was abrogated by the biogenic amine serotonin, acting via the 5-hydroxytryptamine, or serotonin (5-HT1A), subset of serotonin receptors (5-HTR). Thereby, serotonin effectively promoted IFN-gamma production in the presence of monocytes. We conclude that serotonergic 5-HT1A receptors transduce signals that are required for NK cells to produce IFN-gamma in response to IL-2.
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PMID:Role of serotonin in the regulation of interferon-gamma production by human natural killer cells. 845 8

5-Hydroxytryptamine (5-HT) and its receptor have been localized and quantified in the submaxillary gland of rats of various ages, using immunohistochemistry, in situ hybridization and in situ quantification. In male rats, the epithelial cells of serous acini, intercalated ducts, secretary tubes and excretory ducts all showed 5-HT and 5-HT receptor (5-HTR) immunoreactivity. Both 5-HT and 5-HTR reactive sites were found in the same cells of adjacent sections. 5-HT1A receptor mRNA hybridized signals could be detected in cytoplasm of these cells. The parasympathetic ganglia cells and endothelial cells of small vessels also showed 5-HT and 5-HTR immunoreactivity in the cytoplasm. However, in female rats, only the epithelial cells in excretory tubes showed 5-HT and 5-HTR immunoreactivity. The immunoreactivity was present in the same cells of adjacent sections. The relative content of 5-HT and its receptor increased during the first 60 postnatal days but remained constant from day 60 to day 90 postnatum. These results suggest that the submaxillary gland of rats possess autocrine 5-HT, which may regulate the function and development of the gland.
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PMID:Localization and in situ quantification of 5-hydroxytryptamine and its receptor in rat submaxillary gland. 1532 48

Substance P (SP) is a candidate mediator along the brain-skin axis and can mimic the effects of stress to regulate melanogenesis. Previously, we and others have found that the regulation of SP for pigmentary function was mediated by neurokinin 1 receptor (NK1R). Emerging evidence has accumulated that psychologic stress can induce dysfunction in the cutaneous serotonin 5-hydroxytryptamine (5-HT)-5-HT1A/1B receptor system, thereby resulting in skin hypopigmentation. Moreover, NK1R and 5-HTR (except 5-HT3) belong to GPCR. The present study aimed at assessing the possible existence of NK1R-5-HTR interactions and related melanogenic functions. Western blot and PCR detection revealed that SP reduced expression of 5-HT1A receptor via the NK1 receptor. Biochemical analyses showed that NK1R and 5-HT1AR could colocalize and interact in a cell and in the skin. When the N terminus of the NK1R protein was removed NK1R surface targeting was prevented, the interaction between NK1R-5-HT1AR decreased, and the depigmentation caused by SP and WAY100635 could be rescued. Importantly, pharmaceutical coadministration of NK1R agonist (SP) and 5-HT1A antagonist (WAY100635) enhanced the NK1-5-HT1A receptor coimmunoprecipitation along with the depigmentary response. SP and WAY100635 cooperation elicited activation of a signaling cascade (the extracellular, regulated protein kinase p-JNK signaling pathway) and inhibition of p70S6K1 phosphorylation and greatly reduced melanin production in vitro and in vivo in mice and zebrafish. Moreover, the SP-induced depigmentation response did not be occur in 5-htr1aa+/- zebrafish embryos. Taken together, the results of our systemic study increases our knowledge of the roles of NK1R and 5-HT1AR in melanogenesis and provides possible, novel therapeutic strategies for treatment of skin hypo/hyperpigmentation.-Wu, H., Zhao, Y., Huang, Q., Cai, M., Pan, Q., Fu, M., An, X., Xia, Z., Liu, M., Jin, Y., He, L., Shang, J. NK1R/5-HT1AR interaction is related to the regulation of melanogenesis.
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PMID:NK1R/5-HT1AR interaction is related to the regulation of melanogenesis. 2943 Sep 89