Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P06889 (
Mol
)
630,302
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Keratin-associated proteins 9.2 (
KAP9.2
) gene encodes one of the ultra high sulfur KAPs. Variation in KAP genes may affect the structure of KAPs and hence cashmere characteristics. In order to test the association between the polymorphism of
KAP9.2
gene and cashmere trait, DNA sequencing was used to detect a novel C/T polymorphism of
KAP9.2
gene from a genomic DNA pool. The mutation could be recognized by Pst I restriction enzyme. To Shanbei white cashmere goat, Inner Mongolia white cashmere goat and Guanzhong dairy goat, the genotypic frequencies of TT, TC and CC from total 1,236 animals were as follows: 0.047, 0.519 and 0.434; 0.180, 0.592 and 0.228; 0.431, 0.544 and 0.025. The allelic frequencies of T and C were 0.307 and 0.693; 0.476 and 0.524; 0.703 and 0.297, respectively, in breeds mentioned above. The frequency of C allele between cashmere and dairy goat was significant (P < 0.01). To provide support for the hypothesis that SNP 586 was responsible for
KAP9.2
expression, quantitative real-time PCR analysis revealed that the expression level of
KAP9.2
was reduced in individuals bearing genotype CC compared with TT individuals, suggesting that C was the nucleotide causing decreased expression of
KAP9.2
or was in linkage disequilibrium with the causative SNP. The 586C/T SNP found in this study might control translation or stability of
KAP9.2
mRNA, which would be beneficial for marker assistant selection in cashmere goat breeding.
Mol
Biol Rep 2012 Dec
PMID:Variation and expression of KAP9.2 gene affecting cashmere trait in goats. 2305 52
Keratin-associated protein 9.2
(
KAP9.2
) and Homeobox C13 (Hoxc13) genes were chosen to study because of their biological functions involving hair formation.
KAP9.2
gene belongs to the ultra high sulfur KAPs, which is important for hair formation and may have association with cashmere. Hoxc13 takes part in the formation of cashmere keratin and maintaining the normal structure of follicle. It has been reported that Hoxc13 gene exists binding site of KP and KAP genes at its promoter regions in mouse. So the expression of
KAP9.2
and Hoxc13 genes was detected at anagen stage vs telogen stage by qRT-PCR. The data showed that
KAP9.2
and Hoxc13 gene had similar expression trend at different stages, which indicated that there was interaction between them.
KAP9.2
and Hoxc13 gene had lower expression level in anagen than that of in telogen of cashmere growth. In anagen,
KAP9.2
and Hoxc13 expressed lower in high cashmere yield individuals than that of in low cashmere yield ones. In telogen, the result was reverse. The study would provide the evidence of involvement of
KAP9.2
and Hoxc13 in hair periodic growth.
Mol
Biol Rep 2014 Sep
PMID:Expression analysis of KAP9.2 and Hoxc13 genes during different cashmere growth stages by qRT-PCR method. 2490 88
