Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Peptidases capable of releasing proline residues from polypeptides are present in the cytoplasmic fraction of rabbit polymorphonuclear granulocytes. This was shown with peptide substrates where proline is present either at the carboxy-terminal or within the polypeptide chain. Lysosomal and plasma membrane enzymes were inactive towards such polypeptides. The proline residue was hydrolyzed at either its amino end or its carboxy end. It is noteworthy that a Pro:Pro bond was cleaved both in the pentapeptide Thr-Lys-Pro-Arg and the dipeptide Pro:Pro.
Mol Cell Biochem 1976 Feb 16
PMID:Proline endopeptidase and exopeptidase activity in polymorphonuclear granulocytes. 0 27

The formation of compact double-stranded DNA molecules in PEG-containing watersalt solutions (0.3 M NaCl) may be observed within the pH-range 3-10; i.e. under conditions at which parameters of double-stranded DNA helices are not strongly different from those of B-form. At pH less than 3, when regularity of double helices is significantly changed, the formation of the specific compact particles of DNA in PEG-containing solutions does not take place. Denaturation of the compact form of DNA in PEG-containing solution is accompanied by disappearance of the negative band in CD spectrum. Hyperchromic effect of denaturation of DNA compact form is uninformative because of the influence of the light-scattering by compact DNA molecules.
Mol Biol (Mosk)
PMID:[A compact form of DNA in solution. 2. Peculiarities of acidic titration of double-stranded DNA in PEG-containing water-salt solutions]. 0 28

Comparative studies have been carried out for the chemical composition and physico-chemical characteristics of chromatin isolated from spleens of non-immunized and immunized mice. It is found that the chromatin from spleens of immunized mice contains significantly more non-histone proteins and RNA, while the quantity of histone proteins is unaltered. The melting temperatures of chromatin from spleens of non-immunized and immunized mice in 2.5-10(-4) M EDTA (pH 8.0) are 76.8+/-1.50 degrees and 74.4+/-1.10 degrees, respectively. DNA isolated from chromatin melts at 40 degrees. The melting of chromatin was followed in 5 mM sodium-cacodylate buffer (pH 7.0)+1.5-10(-4) M EDTA containing increasing concentrations of urea. The results show that during immunogenesis the changes of the chemical composition of the chromatin are accompanied by certain destabilisation of DNP complex.
Mol Biol (Mosk)
PMID:[Changes in the chemical composition and physico-chemical characteristics of chromatin from spleens of mice during immunogenesis]. 0 29

The pH-dependence of the 3H to 1H exchange between water and C(8)H groups of purine, adenine, 9-methyladenine, 7-methyladenine, hypoxanthine, guanine, xanthine as well as C(2)H groups of imidazole and benzimidazole was studied. It was shown that within the pH-ranges, where the majority of molecules under study are non-ionized, the values of observed rate constant (kobs) do not depend on pH. Beyond these ranges the values of k(obs) are increased or decreased depending on the type of ionizaiton of the compound under study in appropriate pH range. The observed pH dependence of the 3H to 1H exchange is in a good quantitative agreement with ylide mechanism of the exchange reaction. According to this mechanism the 3H--1H exchange takes place in N(7)-protonated forms of the purine derivatives and in zwitterions with positive charge on N(7). The ylide mechanism of the exchange reaction is also suggested by the fact that the true exchange rate constants (k+) of protonated forms of the studied compounds, calculated from the values of k(ods), rises linearly with the increase of their protonation constant (Ka1)--the tenfold increase of Ka1 leads to about four-fold rise of k+. The knowledge of 1h to 3H exchange mechanism in C(8)H groups of purine derivatives allows to estimate alterations of reactivity of the purine residues in polynucleotides and nucleic acids depending on their conformation.
Mol Biol (Mosk)
PMID:[Kinetics and mechanism of the 3H to 1H in C(8)H groups of purine derivatives]. 0 30

1. Seven healthy males were studied during cycle ergometer exercise at 33%, 66% and 90% of VO2 max. on three occasions when NH4C1, NaHCO3 or CaCO3 (as a control substance) were administered in gelatin capsules double blind and in randomized order. Plasma growth hormone (HGH), lactic acid and hydrogen ion concentration ([H+]) were measured at frequent intervals. 2. Ammonium chloride produced highest blood [H+] and NaHCO3 the lowest. These differences were maintained during exercise and in recovery. Plasma lactic acid concentrations were similar at rest. At 66%, 90% VO2 max. and recovery lactic acid was highest with NaHCO3 and lowest with NH4C1. 3. Exercise stimulated HGH secretion in all studies and the elevation was proportional to the intensity of the exercise. NH4C1 caused a variable elevation of HGH at rest and 33% VO2 max. At 66% VO2 max., plasma HGH was significantly elevated to similar concentrations in all studies and, at 90% VO2 max., HGH was highest with NaHCO3. 4. An infusion of sodium L(+)-lactate producing plasma lactate concentrations of 3-5 mmol/l did not influence HGH secretion. 5. Exercise is a physiological stimulus to HGH secretion and the mechanism is independent of blood [H+] and lactate concentrations.
Clin Sci Mol Med 1976 Apr
PMID:Growth hormone secretion in acid-base alterations at rest and during exercise. 0 58

1. Viable human leucocytes were isolated from venous blood and suspended in artificial media. Intracellular pH measurements were made by the dimethyloxazolidinedione technique in conditions simulating "respiratory" or "metabolic" acid-base disturbances. 2. Normal intracellular pH was 7-11 +/- 0-02 (mean +/- 2 SD) at an extracellular PCO2 of 5-8 kPa and a bicarbonate concentration of 25 mmol/l. 3. "Respiratory" and "metabolic" acidosis caused little change in pHi although increases in PCO2 led to relatively greater falls in pHi than did reduction in external bicarbonate concentration. 4. "Respiratory" and "metabolic" alkalosis caused similar and relatively greater increases in the pHi when compared with the response to an external acidosis.
Clin Sci Mol Med 1976 Apr
PMID:The intracellular pH of human leucocytes in response to acid-base changes in vitro. 0 59

Following the demonstration of peptidases in the rat hypothalamus which inactivate thyrotrophin-releasing hormone (TRH), a sensitive and specific radioimmunoassay for the releasing hormone was used to investigate the presence of similar peptidases in the rabbit hypothalamus. TRH was found to be rapidly inactivated by supernatant and particulate hypothalamic fractions, with higher peptidase activity in the supernatant than in the particulate fraction. An optimum pH of 7.3 within physiological limits was obtained for the enzymes in both the fractions examined. The results obtained confirm that the rabbit hypothalamus contains enzymes capable of inactivating TRH, and since it has been found that such peptidases interfere with studies on TRH biosynthesis, it is possible that the peptidases may play a part in controlling the releasing hormone's production. The specificity of the antiserum used in the radioimmunoassay has also suggested that the peptidases may cleave the C-terminal-ProNH2,-NH2 or both from the TRH molecule to cause inactivation.
Mol Cell Endocrinol 1976 Mar
PMID:Hypothalamic inactivation of thyrotrophin-releasing hormone. 0 45


J Mol Biol 1976 Feb 25
PMID:Single-strand breakage on binding of DNA to cells in the genetic transformation of Diplococcus pneumoniae. 0 25


J Mol Evol 1976 Mar 29
PMID:The prebiotic synthesis of deoxythymidine oligonucleotides. III. Acid salt reactions. 0 26

Two strains independently isolated in Salmonella typhimurium display abnormal autolytic activity when nutrient broth becomes alkaline. They also show increased sensitivity to deoxycholate, EDTA, and sodium dodecyl sulfate. Response to acridine orange remains normal. In both strains a single stable mutation is responsible for all the changes. The same gene, called envD, appears to be involved in both mutant strains. envD has been located at minute 33 of the Salmonella genetic map, between markers sucA and nadA, very close to the latter. envD also affects morphological characteristics of the cells. Many mutant cells are shorter than wild type bacteria, and appear frequently associated in short chains of 4 to 10 cells. Furthermore, envD mutants display division by septation under conditions that preclude its observation in wild type strains.
Mol Gen Genet 1976 Feb 27
PMID:Envelope mutation promoting autolysis in Salmonella typhimurium. 0 22


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