Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Cardiac output increased in proportion to oxygen consumption in intact chloralose-anaesthetized dogs after four successive intravenous infusions of 2,4-dinitrophenol (11 mumol/kg; 2 mg/kg). 2. Splenectomy abolished the increase in cardiac output after the first three doses of 2,4-dinitrophenol. beta-Adrenoreceptor blockade by practolol, on the other hand, did not prevent the cardiac output rise after the first 2,4-dinitrophenol infusion, but further increases by 2,4-dinitrophenol infusion were abolished. When splenectomy and beta-adrenoreceptor blockade were combined, cardiac output did not increase significantly after all four doses of 2,4-dinitrophenol. 3. Cardiac output and mean systemic arterial blood pressure increased when the splenic venous blood collected after 2,4-dinitrophenol infusion was infused intraportally. 4. In a vascularly isolated, but normally innervated, lower half-body cross-perfusion preparation, cardiac output and mean systemic arterial blood pressure increased in the upper half-body when tissue hypermetabolism was produced in the cross-perfused area by 2,4-dinitrophenol. Neith pulmonary artery wedge pressure nor heart rate changed significantly. 5. This circulatory stimulation, after regional 2,4-dinitrophenol infusion, was abolished or was prevented from occurring by splenectomy. 6. It appears that the normal cardiac output response to tissue hypermetabolism requires both an intact spleen and normally functioning beta-adrenoreceptors.
Clin Sci Mol Med 1977 Jul
PMID:Regulation of cardiac output during 2,4-dinitrophenol-induced tissue hypermetabolism in the dog. 87 17

1. Oxygen breath tests were performed in seven patients with thyrotoxicosis. The inspired air was replaced by oxygen without their knowledge for 20 s and the fall in ventilation was recorded. 2. Ventilation fell significantly in all the patients within 40 s. The mean fall was 21-1% (se +/-1-7) below the control value. 3. beta-Adrenoreceptor blockade with propranolol had little effect on this reduction in ventilation. The fall observed in five patients receiving propranol was 19-6% (se +/-1-0). 4. After definitive treatment, which rendered the patients euthyroid, the observed reduction in ventilation after breathing oxygen was significantly less at 11-7% (se +/-1-2). 5. It is concluded that patients with thyrotoxicosis have an increased resting reflex hypoxic drive to respiration, when compared with the euthyroid state, and this is unaffected by beta-adrenoreceptor blockade.
Clin Sci Mol Med 1977 Jul
PMID:Effect of thyrotoxicosis on the reflex hypoxic respiratory drive. 87 26

Spontaneously arising erythromycin-resistant mutants were isolated in the "petite-negative" yeast Kluyveromyces lactis. Two independently arising mutants were studied, in one erythromycin-resistance was conferred by a single dominant nuclear gene, and in the other the resistance was extrachromosomally inherited. In fermentable medium growth of sensitive and resistant strains in presence or absence of erythromycin does not qualitatively change the cytochrome absorption spectra, whereas oxygen uptake of parental strains growing in glucose-medium is affected by the drug. The importance of "petite-negative" yeasts like Kluyveromyces lactis for the study of nucleo-cytoplasmic interrelations is discussed.
Mol Gen Genet 1977 Apr 29
PMID:Chromosomal and extrachromosomal inheritance of erythromycin-resistance in the "petite-negative" yeast Kluyveromyces lactis. 87 30

1. Sixteen patients with chronic bronchitis and airways obstruction were given radioactive nitrogen (13N) by intravenous injection and by inhalation, while breathing air and after 10-20 min breathing 30% oxygen. The clearance of 13N from four zones of each patient's whole lung field was monitored. 2. The 13N clearance of each region in these patients with chronic bronchitis was much slower than in normal subjects. Oxygen breathing produced a significant delay in the clearance of intravenously administered 13N in 23 zones in 10 patients but no systematic change in clearance after inhaled 13N. 3. With inhalation of 30% oxygen there was no no significant change in the mean minute ventilation, tidal volume or arterial PCO2. 4. The results suggest that local hypoxic vasoconstriction is present in some patients on breathing air and that this is relieved by 30% oxygen, resulting in a diversion of local blood flow from well-ventilated to more poorly ventilated areas. The fall in VA/Q on 30% oxygen is insufficient to increase arterial PCO2.
Clin Sci Mol Med 1977 Oct
PMID:Effect of 30% oxygen on local matching of perfusion and ventilation in chronic airways obstruction. 91 64

1. Acute hypoxaemia had been reported to stimulate vasopressin release in animals. 2. Hypoxaemia induced by breathing 9-3% oxygen for 15-20 min failed to produce a rise in plasma arginine vasopressin concentration in six out of eight healthy human subjects. The two subjects who developed an increase in plasma arginine vasopressin concentration had a significant rise in serum cortisol. 3. Breathing 100% nitrogen until impairment of consciousness caused no rise in plasma arginine vasopressin concentration.
Clin Sci Mol Med 1977 Oct
PMID:Effect of acute hypoxaemia on plasma arginine vasopressin in conscious man. 91 65

alpha-Hemocyanin from the Roman snail Helix pomatia is composed of polypeptide chains with a molecular weight of 360000 +/- 30000. The cylindrically shaped hemocyanin molecule contains 20 of these large chains. The polypeptide chain has been split into components with molecular weights of: 210000, 154000, 147000, 112000, 120000, 98000, 55000, and 50000, by gentle proteolysis with enzymes of different specificities. Most of the fragments have molecular weights which are about 50000 or a multiple of 50000. Departure from these values, as found in the 112000 and 120000 fragments, is probably caused by the high carbohydrates content of these components. A mixture of these fragments has the same oxygen binding properties as the nondigested protein. Subtilisin converts the hemocyanin polypeptide chain, under appropriate conditions, almost completely into fragments of 50000 and 55000 daltons with conservation of the oxygen binding properties of the nondigested protein. We conclude from these studies that the polypeptide chain of Helix pomatia alpha-hemocyanin is folded into about seven compact tertiary structures, which are covalently interconnected. This chain of structural domains has been visualized. (Siezen and Van Bruggen (1974), J. Mol. Biol. 90, 77-89) by electron microscopy, which shows 1/20 hemocyanin molecules to be flexible structures consisting of 7-8 apparently spherical units of 55-60 A diameter.
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PMID:Proteolytic fragmentation of Helix pomatia alpha-hemocyanin: structural domains in the polypeptide chain. 93 32

1. Oxygen breath tests were performed in nine patients with diffuse pulmonary infiltration whose resting arterial PO2 (Pa, O2) ranged from 8-9 kPa to 11-8 kPa. The inspired air was suddenly replaced with oxygen for 30 s and the percentage fall in ventilation over the last 10 s was measured. 2. Pa,O2 rose rapidly and exceeded 16 kPa within 20 s in each patient. 3. The ventilation fell significantly in seven of the nine patients. It is concluded that these seven patients had a demonstrable reflex hypoxic respiratory drive at rest. This tended to be greatest in patients with the lowest Pa, O2. The percentage falls in ventilation observed were similar to those previously reported at comparable Pa, O2 levels in patients with chronic bronchitis. 4. The resting arterial PCO2 (Pa,CO2) ranged from 5-0 to 5-8 kPa. It did not change by more than 0-3 kPa during the oxygen breath tests in any patient.
Clin Sci Mol Med 1976 Feb
PMID:Estimation of the resting reflex hypoxic drive to respiration in patients with diffuse pulmonary infiltration. 94 73

1.Gas-density-dependence of maximal expiratory flow rats (V max), defined as the ratio of V max. while breathing helium/oxygen (80:20) to V max. while breathing air at the same lung volume, was examined in relation to other measurements of airways obstruction in patients with obstructive airways disease before and after administration of bronchodilators. 2. Seventeen patients showed a 45% or greater increase in specific conductance (sG aw) after bronchodilator therapy (group A) and thirteen patients demonstrated a lesser response (group B). 3. Before the administration of bronchodilators, the degree of obstruction in two groups was not different as measured by lung volumes, sG aw, forced expiratory volume in 1 s, and flow rates high in the vital capacity; yet the maximal mid-expiratory flow rate and the degree of density-dependence were significantly lower in group B. 4. After bronchodilators, both groups of patients showed significant improvements in sG aw, flow rates and lung volumes. However, group A patients showed a signifcant increase in density-dependence whereas group B patients did not. 5. Increased density-dependence after bronchodilators in the group A patients was associated with an increase in the computed resistance of the upstream segment with air and a decrease in resistance with helium/oxygen. These changes could be explained by a more mouthward of equal pressure points, and therefore a further increase in the relative contribution of the larger density-dependent airways to limitation of flow. 6. The fact that density-dependence was not altered after bronchodilators in the group B patients suggests that the site of limitation of flow did not change appreciably. The shift in the pressure-flow curve for the upstream airways was such that the computed resistance of these airways fell. Thus it appears that the airways comprising the upstream segment were dilated.
Clin Sci Mol Med 1976 Aug
PMID:Density-dependence of maximal expiratory flow rates before and after bronchodilators in patients with obstructive airways disease. 95 59

1. Physiological dead-space volume (V D) was measured in twenty-four healthy men and women aged from 20 to 71 years, at rest and at two rates of work on a treadmill, whilst breathing air and breathing oxygen. 2. The effect of correction of arterial carbon dioxide tension (Pa,CO2) to pulmonary capillary temperature on the resulting value for V D was investigated. We find that the effect is substantial and that a correction should be made. 3. Equations have been derived for the prediction of normal V D during exercise. The best prediction was given by a regression on height, age, carbon dioxide output, ventilation and respiratory frequency, with an upper 95% confidence limit of +81 ml.
Clin Sci Mol Med 1976 Oct
PMID:Gas exchange during exercise in healthy people. I. The physiological dead-space volume. 97 73

1. Venous admixture/cardiac output ratio (Qva/Qt) has been measured in twenty-four healthy volunteer subjects of both sexes aged 20-71 years, at rest and during the steady state of treadmill exercise at two rates of work, and breathing air and breathing oxygen. 2. With oxygen breathing, Qva/Qt was considerably less during exercise than during the time subjects were taking either normal or deep breaths of oxygen at rest, and did not significantly increase with the intensity of exercise. It is postulated that the increase in ventilation during exercise opens most or all of those alveoli which, during oxygen breathing at rest, close because of critically low ventilation/perfusion (V/Q) ratios. 3. With air breathing, Qva/Qt fell from rest to exercise (especially in older subjects), presumably due to improved ventilation of alveoli at the lung bases. With an increase in work rate Qva/Qt increased in all age groups. This increase was not due to increase in the shunt fraction (Qs/Qt), nor to limitation of diffusing capacity; it arose from an increase in V/Q variance. 4. Equations have been derived for the prediction of normal Qva/Qt during exercise, with or without correction for the effects of increasing pulmonary capillary temperature. These effects do not materially influence the accuracy of prediction, but may be relevant to some of the interpretations. In particular, they provide a further indication that Qs/Qt probably cannot be measured by breathing oxygen at rest, even in deep breathing.
Clin Sci Mol Med 1976 Oct
PMID:Gas exchange during exercise in healthy people II. Venous admixture. 97 74


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