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Query: UNIPROT:P06889 (Mol)
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1. Acute chloride depletion, without sodium depletion, was produced in rats by a single exchange peritoneal dialysis against sodium bicarbonate solution. Blood volume was restored after dialysis by infusion of salt-free albumin, and exogenous deoxycorticosterone and antidiuretic hormone were given. 2. Clearance studies in the period (3 h) after dialysis revealed no difference in the glomerular filtration rate or in the filtered sodium load between experimental and control rats but urinary sodium concentrations and absolute and fractional sodium excretion were significantly higher in the chloride-depleted group. 3. There was also a significant kaliuresis, increased urinary flow rate and diminished free water reabsorption. Urinary bicarbonate excretion increased to a variable degree but the major rise in anion excretion was 'unmeasured' (Na+ + K+ - [Cl- + HCO3- + PO4(3-)]). 4. It is postulated that chloride depletion imposes limitations on sodium reabsorption in the ascending limb of the loop of Henle.
Clin Sci Mol Med 1977 Jan
PMID:Natriuresis in rats acutely depleted of chloride. 60 61

Analysis of experimental data shows that interaction of charges in an aqueous medium is satisfactorily evaluated by macroscopic dielectric water permeability even at small (approximately 3--4 A) distances. It has been shown that a non-polar molecule located between the charges weakens their interaction. At the same time interaction between charges situated on the non-polar molecules somewhat increases. An estimate is made of the free energy loss of alpha- and beta-structures of polypeptides, resulting from the insertion of charged groups as well as of the free energy of interactions involving charged groups in different secondary structures.
Mol Biol (Mosk)
PMID:[Electrostatic interactions of charged groups in an aqueous medium and their effect on the formation of the secondary structures of polypeptide chains]. 61 25

In the paper results are presented of investigation of protochlorophyll (PChl) and chlorophyll (Chl) mixed associations and of interaction between them within the polymer molecular complex, which forms in mixture of water-dioxane (1 : 4). The initial PChl concentration in all solutions was constant (CPChl = 1 . 10(-5) m/l), and Chl concentration varied from 1.10(-8) m/l up to 2.10(-5) m/l. It is shown that with the rise of Chl proportion in the mixed aggregate the rearrangement of both donor (PChl) and acceptor (Chl) components of complex takes place. The luminescence quenching of PChl and the sensitization of Chl emission in mixed pigment associates were investigated of different Chl levels and the evaluation of efficiency of intracomplex electronic excitation energy transfer, determined from quenching and sensibilization, was performed. Similar dependence of energy transfer effectiveness on Chl concentration, determined by the two above-mentioned methods shows that the excitation migration in an associate takes place without losses. An analysis of results permits to conclude that a small trapping efficiency of PChl excitation by the acceptor part of the complex may be connected with the existence of the prerelaxation reverse energy transfer from Chl to PChl in mixed pigment associates. On the basis of the obtained data a mechanism of energy transfer from protochlorophyllide to chlorophyllide in etiolated leaves and homogenates is discussed.
Mol Biol (Mosk)
PMID:[Relationship between chlorophyll concentration and the energy reaction between protochlorophyll and chlorophyll in mixed associations]. 61 38

A nomograph is suggested using which one can determine from the molecular weight and one of the dimensions of the molecule the remaining dimension and the axial ratio for the ellipsoid of rotation with the volume equivalent to the volume of the protein molecule. The nomograph permits to determine molecular weight from the molecular size of the dry protein and of the protein dissolved in water. From the molecular size of the dry protein corresponding dimensions for the protein molecule in an aqueous solution can be obtained. At last the nomograph can help to evaluate the correctness of the literature data and experimental data on the molecular sizes of proteins and on their molecular weights.
Mol Biol (Mosk)
PMID:[Nomogram for the determination of the size of protein molecules and approximation of their molecular weights]. 61 43

Generation of electric potential difference by bacteriorhodopsin proteoliposomes incorporated into the phospholipid-impregnated collodion film has been studied. It is shown that illumination of this film by continuous light gives rise to the generation of an electric potential difference across the film (plus on the bacteriorhodopsin-free side), which can be as high as 300 mV. Short unsaturating flash inducing single turn-over of bacteriorhodopsin generates the potential difference which is a function of the flash intensity (70 mV at 3 mjoule light). The flash-induced photoelectric response consists of four phases. (1) Very fast (tau less than 1 microsec) generation of a potential difference (minus in the bacteriorhodopsin-free compartment). The amplitude of this phase is rather small (1--5 mV). (2) Fast phase of positive charging of the bacteriorhodopsin-free compartment (tau = 25--50 microsec). (3) Slow phase of positive charging of this compartment (tau = 6--12 msec) Amplitude of the second phase is to that of the third as 1 : 2. (4) A very slow phase of discharge of the flash-induced potential difference (tau = 1 sec at 10(8) ohm X cm2 film resistance). The third phase was specifically inhibited by La3+. Both the second and the third phases are decelerated by substitution of D2O in 4.5--5 and 2 times, respectively, while the amplitude of the first phase increases. Prolonged storage of the system in the dark (tua = 20--25 min) causes the decrease in the amplitudes of the second and the third phases as if the amount of active bacteriorhodopsin molecules were increased by factor 2. Such an inhibition was reversed by 30--60 sec illumination of the system. The dark adaptation is accompanied by some increase in the first phase amplitude. Comparison of these data with results of other studies on bacteriorhodopsin suggests that (1) the first phase is due to the photoinduced change in the retinal dipole; (2) the second phase corresponds to H+ transfer from Schiff base to the water solution in the proteoliposome interior; 3) the third phase represents H+ transfer from the incubation mixture to Schiff base; (4) the dark adaptation is a result of transition of photoelectrochemically active all-trans-retinal to the inactive 13-cis-retinal.
Mol Biol (Mosk)
PMID:[Temporal characteristics of bacteriorhodopsin as a molecular biological generator of current]. 61 49

1. We measured urinary kallikrein (kininogenin) excretion in black and white normotensive subjects during a variety of manipulations of salt and water balance. 2. A large intravenous saline load administered while the subjects were on an unrestricted sodium diet did not significantly change urinary kallikrein activity in either racial group. 3. After several days of dietary sodium restriction both racial groups increased their urinary kallikrein activity. An intravenous water load given then further increased urinary kallikrein activity. White subjects were studied for an additional 24 h period, and urinary kallikrein activity returned to pre-water load values, indicating that the excretion of a water load in sodium-depleted subjects is associated with an increase in kallikrein excretion. 4. Black subjects excreted less kallikrein in the urine than white subjects during the initial 24 h periods of unrestricted dietary sodium intake, but there were no other significant racial differences during the other experimental conditions.
Clin Sci Mol Med 1978 Jan
PMID:The relationship of urinary kallikrein activity to renal salt and water excretion. 62 Apr 92

1. Free amino acids were determined in the plasma and in the muscle tissue of 14 patients with chronic uraemia; eight were not on dialysis and six were having regular peritoneal dialysis. The concentration of each amino acid in muscle water was calculated with the chloride method. 2. In both groups of patients there were low intracellular concentrations of threonine, valine, tyrosine and carnosine, and high glycine/valine and phenylalanine/tyrosine ratios. Both groups of patients had increased amounts of 1- and 3-methyl-histidine in plasma and in muscle water. 3. The non-dialysed patients had low intracellular concentrations of lysine, and the dialysed patients had high intracellular concentrations of lysine, isoleucine, leucine and of some of the non-essential amino acids. 4. After peritoneal dialysis for 22 h, the plasma concentration of several amino acids decreased but the intracellular concentrations of most amino acids did not change significantly. 5. Intravenous administration of essential amino acids and histidine during the last 4 h of dialysis increased in muscle the total free amino acids, the ratio of essential to non-essential amino acids and the valine and phenylalanine concentrations. 6. The results demonstrated that the plasma and muscle concentrations of several amino acids are grossly abnormal in chronic uraemia. Non-dialysed and dialysed patients exhibit important differences, especially in the intracellular amino acid patterns. Infusion of essential amino acids may result in enhancement of protein synthesis.
Clin Sci Mol Med 1978 Jan
PMID:Intracellular free amino acids in muscle tissue of patients with chronic uraemia: effect of peritoneal dialysis and infusion of essential amino acids. 62 Apr 93

1. The effect of extracellular volume expansion (ECVE) during water diuresis, and of water diuresis alone, on the formation of free water in man was compared. 2. ECVE reduced free water formation at any given rate of distal delivery compared with water diuresis. Thus, ECVE depresses distal sodium chloride reabsorption. 3. This attenuation of free water formation occurred both when urine flow (V/100 ml glomerular filtration rate) and distal chloride delivery [(Cwater + Ccl)/100 ml glomerular filtration rate] were used as the terms for distal delivery. 4. We suggest that the distal depression of sodium chloride reabsorption after ECVE is robably due to a direct inhibition of distal sodium chloride transport mechanisms, and not to the flooding of the diluting site by the poorly reabsorbable bicarbonate ion.
Clin Sci Mol Med 1978 Mar
PMID:The effect of acute extracellular volume expansion on sodium chloride reabsorption in the diluting segment in man. 63 Aug 10

1. Glucose absorption, water absorption and dipeptide hydrolase activities have been determined in isolated rat small intestine at 1, 3, 5 and 21 days after a single intraperitoneal injection of 5-fluorouracil. 2. Absorption rates and enzyme activities were elevated 1 day after treatment, but were reduced to 40% of control values at 3 and 5 days. Changes were seen regardless of whether absorption was expressed per unit length or per unit dry weight of intestine. 3. There were highly significant positive correlations between glucose or water absorption rates and peptidase activities, especially in proximal jejunum. The most significant correlation was observed between water absorption rate and jejunal L-Leu-Gly hydrolase activity. 4. Malabsorption may account for some of the gastrointestinal side effects associated with treatment with 5-fluorouracil. Enzyme measurements may be useful as an index of intestinal function.
Clin Sci Mol Med 1978 Apr
PMID:Changes in absorptive and peptide hydrolase activities in rat small intestine after administration of 5-fluorouracil. 63 72

1. Five normal subjects were studied before and during treatment with carbamazepine. 2. Plasma sodium, plasma and urine arginine-vasopressin and urine osmolality were measured during a day of water deprivation, before and during drug treatment. 3. During treatment with carbamazepine plasma sodium increased wheras plasma and urine arginine-vasopressin and urine osmolality decreased. Plasma and urine arginine-vasopressin were signifi"antly correlated with urine osmolality. However, carbamazepine did not affect the osmolality of urine produced by the kidney, in response to endogenous arginine-vasopressin. 4. Plasma and urine arginine-vasopressin were significantly correlated with plasma sodium on both control and drug-treatment days, but the relationships of plasma and urine arginine-vasopressin to plasma sodium were different during carbamazepine treatment, as compared with the control period. 5. It is suggested that the threshold of the hypothalamic osmoreceptors for release of arginine-vasopressin is modified by carbamazepine, and that this may be either a direct action or secondary to another action of the drug.
Clin Sci Mol Med 1978 Apr
PMID:Effect of carbamazepine on plasma and urine arginine-vasopressin. 63 73


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