Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
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The complex proton spin-echo decay curve was recorded for human serum albumin (SA) solutions with different concentrations in normal and heavy water. The curve included three fast-decaying components for SA, in addition to the slow-decaying component for the water. The total amplitude of these three components roughly corresponded to the number of protons in the SA (with isotopic exchange taken into account); the component ratio remained constant at different concentrations and different temperatures between 4 and 39 degrees. The relatively slow-decaying protein component, which accounted for similar to 10% of the SA protons, was produced by the side chains of the protein. The presence of two other faster-decaying SA components with approximately equal amplitudes indicated that only about half of the remaining protons in the SA macromolecule are incorporated into the comparatively rigid globule, the other half belonging to groups with high conformational lability in aqueous solution. The activation energy for the aqueous component was close to that for pure water, while the activation energies for the protein components were roughly twice as large.
Mol Biol 1975 Jan
PMID:Investigation of the conformational lability of serum albumin macromolecules in aqueous solution by the NMR spin-echo method. 23 8

1. Synthesis of several pepstatin A derivatives was performed with the aim of increasing water solubility without altering the capacity to inhibit the renin-angiotensinogen reaction. 2. Pepstatinyl-arginine-O-methyl ester was studied in vitro and in vivo and compared with pepstatin A and with the arginine salt of pepstatin A. 3. This compound inhibited in vitro the reaction between purified hog renin and the synthetic renin N-acetyl-tetradecapeptide or the natural rat renin substrate. The inhibitory constant was of the same order of magnitude as that of pepstatin A. 4. In renal hypertensive rats, the bolus injection of pepstatinyl-arginine-O-methyl-ester or of the arginine salt of pepstatin decreased blood pressure to the same extent as a bolus injection of Sar1, Ala8-angiotensin II.
Clin Sci Mol Med Suppl 1978 Dec
PMID:Soluble pepstatins: a new approach to blockade in vivo of the renin-angiotensin system. 28 46

1. In normally hydrated rats prostaglandin F2alpha (PGF2alpha) in doses of 5 microgram/100 g body weight given subcutaneously every 2 h (three times) induced a significant increase in urinary kallikrein activity, and in sodium, potassium and water excretion for 8 h after the first injection. In moderately hyperhydrated rats loaded 2.5% of body wt. with 0.5% NaCl solution, PGF2alpha produced similar changes in kallikrein activity and electrolyte excretion. 2. In normally hydrated rats prostaglandin E2 (PGE2) in the same conditions and doses as in 1 had no effect on kallikrein activity, showing a tendency to decrease potassium and water excretion. 3. PGE2 in doses of 5, 12.5 and 25 microgram/100 g body wt. in overhydrated rats given 2.5% and 0.5% NaCl and 5% of tap water/100 g body wt. 1 h later, significantly increased kallikrein activity in the urine collected for 120 min after the injections. A significant decrease in potassium and water excretion was observed with the highest dose. 4. PGF2alpha, had no effect on kallikrein activity in overhydrated rats, but an increase in sodium and a decrease in potassium excretion was seen at the highest dose. 5. The different actions of PGE2 and PGF2alpha may be part of a regulatory mechanism associated with the kallikrein-kinin system which contributes maintainance of extracellular fluid homeostasis.
Clin Sci Mol Med Suppl 1978 Dec
PMID:Effects of prostaglandin E2 and prostaglandin F2alpha upon urinary kallikrein excretion in rats. 28 49

1. Indomethacin was administered alone or in addition to either diuretic or propranolol therapy to three groups of patients with essential hypertension on a free sodium diet. 2. Indomethacin administration reduced renin secretion by about 30% in untreated uncomplicated hypertensive patients and by about 75% in those whose renin secretion had either been stimulated or suppressed by maintained diuretic or beta-adrenoreceptor-blockade therapy. 3. Indomethacin administration produced no net effect on blood pressure in untreated patients with uncomplicated hypertension but it blunted or reversed the antihypertensive effect of either diuretic or propranolol therapy. 4. Salt and water retention may be an important factor in the blood pressure-raising effect of indomethacin during diuretic or propranolol therapy: In addition, prostaglandin synthesis may be important in counteracting increased alpha-adrenergic tone, which may limit the blood pressure-lowering effect of beta-adrenoreceptor-blockade. 5. Because of these interactions and their pressor potential indomethacin should be used with caution when combined with either diuretics or beta-adrenoreceptor blockers.
Clin Sci Mol Med Suppl 1978 Dec
PMID:Effects of indomethacin alone and during diuretic or beta-adrenoreceptor-blockade therapy on blood pressure and the renin system in essential hypertension. 28 51

The formation of compact particles from double-stranded DNA molecules in water-salt solutions containing spermidine was studied. It has been shown that in solutions of low ionic strength (0.01 M NaCl) DNA-spermidine complexes have the form of large particles which scatter UV-light. Electron micrographs show that such complexes formed at certain molar ratios spermidine/DNA may exist both as intermolecular aggregates and as toroidal particles 1500 A in diameter. The CD spectra of solutions containing DNA-spermidine complexes are characterized by the positive band (delta epsilon max = 10) at 265--270 nm. The appearance of the positive CD band may be caused by two factors: interaction between DNA and spermidine may lead to the alteration of the DNA secondary structure "in direction to A-form" or intermolecular aggregation, which may change the initial shape of the CD spectrum. The exclusion of spermidine molecules from DNA-spermidine complexes by Na+ ions in presence of poly(ethylene glycol) which occurs as the ionic strength increases from 0.01 to 0.3 does not lead to decompactization of DNA molecules but is accompained by the appearance of the intense negative CD band at 270 nm.
Mol Biol (Mosk)
PMID:[Formation of the compact form of DNA in solution after reaction with spermidine]. 34 64

The interaction of water molecules from the vapour phase with the total backer's yeast tRNA preparation was studied by the dynamic aquametric method. The primary hydration sites for processes of sorption and desorption of water molecules was evaluated by means of multilayer adsorption BET-equation. It was shown that the primary hydration sites are the oxygen atoms in the ribose-phosphate backbone of the tRNA molecule. The structure of surfaces of globular proteins and tRNA molecules were compared from the point of view of their ability to interact with water molecules. The higher degree of maximal hydration (under saturated water vapour or in aqueous solution) was considered as a result of regular arrangement of the most part of tRNA primary hydration sites.
Mol Biol (Mosk)
PMID:[Hydration of a preparation of total yeast tRNA]. 37 64

The present work is concerned with a sensitive and fast micromethod for separation of single- and double-stranded molecules of nucleic acid by hydroxyapatite (HAP) thin-layer chromatography. The thin layers were obtained by precipitation of ground HAP particules into the surface of the plates in water. Chromatography in sodium phosphate buffer makes it possible to separate from 1 to 50 micrograms of nucleic acids for 30--50 sec. Thereby double-stranded molecules remain at the starting line, whereas single-stranded DNA or RNA follow up the solvent. For quantitative assay of nucleic acids by HAP thin-layer chromatography, the plates were scanned in UV light, radioactivity was measured without extracting substances from HAP and DNA and RNA were eluted with the help of phosphate buffer. A simple and accurate determination method has been suggested consisting in dissolving HAP in perchloric acid followed by hydrolysis of nucleic acids and spectrophotometry of solutions. The retrieval of the material after chromatography in 99 +/- 2%, the mean determinations error is 2--3%. The conditions are described for extraction, after thin-layer chromatography, of desalted and concentrated DNA, ready for use in later experiments. The paper describes a method: for determination of the degree of DNA nativity; quantitative determination of DNA in solutions, containing admixtures; separation of synthesized RNA from its precursors and from the DNA template; assay of DNA thermostability; investigation of the kinetics of DNA reassociation and DNA-DNA hybridization. Some results obtained from hydroxyapatite thin-layer chromatography are discussed.
Mol Biol (Mosk)
PMID:[Hydroxyapatite thin-layer chromatography of nucleic acid]. 37 2

The effect of ozone exposure on Saccharomyces cerevisiae was studied. Factors such as ozone concentration, treatment time, media, initial cell concentration and growth phase were shown to influence ozone response in this organism. Logarithmic phase cells were much more sensitive than stationary phase cells to the lethal effect of ozone. The radiation-sensitive mutants rad3, rad6, rad51 and rad52 of S. cerevisiae were exposed, in water, to 50 ppm of ozone for 30 min. On comparing their survival curves, the rad51 and the rad52 mutants showed a greater sensitivity to ozone exposure than the wild type.
Mol Gen Genet 1979 Nov
PMID:Ozone response in wild type and radiation-sensitive mutants of Saccharomyces cerevisiae. 39 40

1. The effect of oral administration of an inhibitor of prostaglandin synthetase, indomethacin, on the natriuresis induced by the infusion of sodium chloride (saline) was studied in 11 healthy volunteers. 2. The administration of indomethacin did not alter sodium excretion before saline infusion, but it resulted in a significant increase of the natriuresis after saline infusion. This increase was not accompanied by any change in post-infusion urine flow rate or free water reabsorption. 3. It is suggested that intrarenal prostaglandins might suppress the natriuretic effect of saline infusion, probably by increasing sodium reabsorption in the distal nephron.
Clin Sci Mol Med 1978 Jan
PMID:Effect of inhibition of prostaglandin synthesis on the natriuresis induced by saline infusion in man. 41 87

The day kaolinite was tested for its ability to promote nucleotide oligomerization in model prebiotic systems. Heterogeneous mixtures of clay, water and nucleotide were repeatedly evaporated to dryness at 60 degrees C and redissolved in water in cyclic fashion in the presence or absence of cyanamide and/or ammonium chloride. With or without cycling, kaolinite alone did not promote the oligomerization of nucleotides at detectable levels. Cycling of clay in combination with cyanamide, however, promoted high levels of condensation to a mixture of oligonucleotides and dinucleotide pyrophosphate without requiring ammonium chloride. Although cycling with clay favored synthesis of dinucleotide pyrophosphate, cycling without clay enhanced formation of oligonucleotides. These results support the hypothesis that the presence of clays in fluctuating environments would have influenced the -ourse of prebiotic condensation reactions.
J Mol Evol 1979 Mar 15
PMID:Prebiotic nucleotide oligomerization in a fluctuating environment: effects of kaolinite and cyanamide. 43 48


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