Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

During progression and in the early phase on a regression regimen, calcification of the necrotic portion of the atheroma of swine abdominal aorta occurred primarily in degenerated cells or in membranous, vesicular cellular degradation products which varied in size, shape, and the amount of mineral deposit. Calcium appeared to be deposited in amorphous granular or needle-like crystalline forms. Energy dispersive X-ray and line profile analysis showed that the major elements in the heavily calcified portions of the plaques were calcium and phosphorus. There was a direct relationship between the distribution and concentration of these elements indicating that the mineral deposit was a calcium phosphate. Select area electron diffraction analysis of grossly calcified portions of the plaque gave a diffraction pattern identical to that of calcium hydroxyapatite. Calcification was not observed to occur on elastic tissue or collagen fibers.
Exp Mol Pathol 1985 Dec
PMID:Ultrastructural and elemental analysis of calcification of advanced swine aortic atherosclerosis. 406 12

Treatment of gp18, a biologically active monomer of the structural protein of the bacteriophage T4 contractile sheath, with 0.6 M-HClO4 leads to the release of GDP, GMP and inorganic phosphate. Each gp18 molecule is shown to carry three atoms of phosphorus. In the isolated protein preparation, gp18 and the nucleoside phosphate are in equimolar relation. It is suggested that in the native sheath-protein subunit, GDP and inorganic phosphate are united as GTP.
J Mol Biol 1984 Nov 05
PMID:On the presence of guanosine phosphate in the tail of bacteriophage T4. 609 55

Aurosomes produced in the rabbit synovial membrane after intraarticular injection of colloidal gold were found to contain large spherical electron-dense granules and fine electron-dense particles. Electron-probe x-ray analysis demonstrated the presence of gold in the granules and iron in the particles. Sulphur and phosphorus were not detected in these aurosomes produced by colloidal gold. This is in contrast to the aurosomes produced by the soluble gold salt sodium aurothiomalate where besides gold, sulphur and phosphorus are easily detected.
Virchows Arch B Cell Pathol Incl Mol Pathol 1980
PMID:Electron-probe x-ray analysis of granules and particles found in aurosomes produced by colloidal gold. 610 25

The lipid composition of rat spinal cord undergoing postmortem autolysis for 3 min and for 4 h at 38 degrees C was investigated as a model for lipid changes in total spinal cord ischemia. The only change in cords incubated for 3 min was an 11.7% decrease in cholesterol/g fresh weight. The cords incubated for 4 h showed a similar 11.6% decrease in cholesterol/g fresh weight as well as a 5.6% increase in water content and a 22% decrease in phosphatidyl serine. Changes of marginal statistical significance included a 15% increase in lipid phosphorus/g dry wt. and a 15% decrease in G4 (GM1)1 in the 4 h incubated cords. Therefore, autolytic processes are of little consequence in total spinal cord ischemia and attention should now be focused on exogenous pathogenetic factors to explain such ischemic changes in spinal cord. We also report discovery of an alkali-labile ganglioside, G1a in rat spinal cord.
Virchows Arch B Cell Pathol Incl Mol Pathol 1981
PMID:Effects of postmortem autolysis on the lipids of rat spinal cord. 611 47

Cultured rabbit kidney cells were exposed to uranyl acetate. This produced single-membrane-bound presumably lysosomal bodies (called 'uraniosomes') containing electron-dense crystals in the cultured cells. Similar crystalline deposits were seen in extracellular locations also. All uraniosomes and extracellular uranium deposits analyzed by electron-probe x-ray analysis were found to contain uranium, potassium, calcium and phosphorus. Traces of sulphur were detected in some but not all uraniosomes and extracellular uranium deposits.
Virchows Arch B Cell Pathol Incl Mol Pathol 1982
PMID:Uraniosomes produced in cultured rabbit kidney cells by uranyl acetate. 612 78

We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum], EC 3.1.3.2) in Saccharomyces cerevisiae at the physiological and molecular levels, through a series of repression and derepression experiments. We demonstrated that APase synthesis is tightly regulated throughout the growth phase and is influenced by exogenous and endogenous Pi pools. During growth in a nonlimiting Pi medium, APase is repressed. When external Pi becomes limiting, there is a biphasic appearance of APase mRNA and enzyme. Our data on APase mRNA half-lives and on the flux of intracellular Pi and polyphosphate during derepression are consistent with a mechanism of transcriptional autoregulation for the biphasic appearance of APase mRNA. Accordingly, preculture concentrations of Pi control the level of corepressor generated from intracellular polyphosphate degradation. When cells are fully derepressed, APase mRNA levels are constant, and the maximal linear accumulation rate of APase is observed. A scheme to integrate phosphorus metabolism and phosphatase regulation in S. cerevisiae is proposed.
Mol Cell Biol 1983 May
PMID:Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae. 634 58

Diets containing alkali-treated soy protein have been shown to cause nephrocalcinosis in rats. In order to determine if alkali-treated soy protein is the dietary component that induces nephrocalcinosis, the effects of a purified diet containing 20% alpha-protein (an alkali-treated soy protein) were compared with the effects of the same diet containing 20% promine-D (a non-alkali-treated soy protein) on renal morphology and renal calcium and phosphorus metabolism. After a 9-week feeding trial, light and transmission electron microscopy revealed that the animals fed either the alpha-protein or promine-D diet developed nephrocalcinosis. In fact, the type of nephrocalcinosis was the same in both groups of animals. Moreover, quantitative determinations of total renal calcium and phosphorus showed that the severity of nephrocalcinosis was also the same in the two groups. No signs of nephrocalcinosis were detected in rats fed a standard commercial laboratory diet. Since nephrocalcinosis was present in the animals fed the promine-D diet, and that it was identical to that found in the animals fed the alpha-protein diet, it appears that alkali-treated soy protein is not the factor responsible for nephrocalcinosis in rats fed a diet containing the protein.
Exp Mol Pathol 1984 Oct
PMID:Nephrocalcinosis and diets containing alkali- and non-alkali-treated soy protein. 654 Nov 56

A high incidence of spontaneously formed urinary stone was found in the females of a jaundiced strain of rat developed from a cross between Gunn's rat and Wistar-Imamichi rat. In this colony, 42.3% of the females had urinary calculi. Elemental analyses of these urinary calculi were carried out with an analytical electron microscope, a high-resolution transmission electron microscope fitted with an energy dispersive type X-ray microanalyzer and a scanning device. In the surface and middle areas of the stone, the main components were recognized as magnesium (Mg) and phosphorus (P). In the central region of the stone, calcium (Ca) and phosphorus (P) were found as the main elements with trace amounts of sodium (Na), chlorine (Cl), and potassium (K). The analyses indicated that the spontaneous urinary stone consisted of phosphate salts with calcium or magnesium. In addition, the mass ratio of the Mg/P or Ca/P in the stones was calculated from X-ray pulse intensity ratios and compared with the mass ratio of a standard sample. The results suggested that the magnesium and phosphorus in the urinary stones existed as ammonium magnesium phosphate, MgNH4PO4, and the calcium and phosphorus as tribasic calcium phosphate, Ca3(PO4)2.
Exp Mol Pathol 1984 Feb
PMID:X-ray microanalysis of spontaneously formed urinary calculi in a jaundiced strain of rat. 669 2

The affinity labeling of E. coli RNA polymerase by periodate oxidized uridine triphosphate was carried out without and with the template (polydeoxynucleotides poly(dA) and poly(dT) ), and under the conditions of poly(dA) and poly(dT) transcription. The extent of RNA polymerase labeling was nearly the same in the presence of poly(dA) and poly(dT) (0.9 and 0.7). However, during the transcription of poly(dA) the extent of RNA polymerase labeling proved to be more than twice as high as that in the case of poly(dT) (0.75 and 0.3). The longitudinal relaxation times (T1) for phosphorus of ATP and dinucleotide pUpU in the complexes of RNA polymerase with the template (poly(dA) and poly(dT) in the presence of MnCl2 were determined. The distance from the phosphorus of enzyme-bound ATP and pUpU and the Mn2+ ion coordinated at the active center on RNA polymerase were calculated using the Solomon - Blombergen formula. According to the results of the affinity labeling of RNA polymerase and the NMR (nuclear magnetic resonance) experiments it can be inferred that the template influences the formation of the working conformation of the enzyme active center, specific with respect to the true substrate, in the stage of RNA elongation.
Mol Biol (Mosk)
PMID:[Interaction of RNA polymerase of Escherichia coli with substrate by affinity labeling and NMR with nuclear 31P]. 675 Mar 60

Equivalent conductivity (lambda MeP) was studied for the Li+, Na+, K+, Cs+, NH4+ salts of poly(U) and for polyribouridilic acid. Concentration of the salt free solutions of poly(U) ranges from 1 . 10(-4) M-3 . 10(-3) M. Limiting equivalent conductivity (lambda 0 MeP) was obtained based on linear dependence of lambda MeP on Cp1/2 (where Cp is the concentration of nucleic phosphorus). The lambda 0 MeP values obtained were shown to increase linearly with the increase of the limiting mobility of counterion. These data were used to calculate limiting mobility of macroion (lambda p = 43 Ohm-1 Cm2 equiv-1) and parameter xi = 1.13 which characterizes charge density on a macroion. Linear dependence was shown to take place for poly(U) acid and it's salts in the Ostwald's coordinates; moreover, pK value for the phosphate groups is practically independent of the counterion's nature and equal 1.93 +/- 0.32.
Mol Biol (Mosk)
PMID:[Structural parameters of charge density, macroion mobility and dissociation constants of the phosphate groups from conductivity measurements of salt free solutions of poly(U) and its salt]. 685 64


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