Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

By measuring DNA temperature transition profiles the evidence was obtained that DNA of phages Tg9 and OB infecting Bacillus thuringiensis and Brevibacterium flavum respectively have a random base distribution. After alkaline treatment in the presence of 10 per cent formaldehyde at room temperature partially denatured molecules of phage Tg9 and OB DNA's with different degrees of denaturation were obtained. Using electron microscopy the maps of distribution of melted regions along these DNA's were obtained. The location of the main peaks on histograms did not vary with the increase of the degree denaturation and changes of pH values. The map of Tg9 DNA is characterized by four main peaks located at the positions 0.031, 0.075, 0.145 and 0.885 but the map of OB DBA is characterized by seven main peaks located at positions: 0.09, 0.20, 0.34 0.42,0.49, 0.80 and 0.88 from the left end (in relative units). The increase of denaturation up to a certain value did not change the half-width of the main peaks at the maps of these DNA's. It may be due to the presence in the DNA's some "heat stolle" sequences limiting early melting DNA regions.
Mol Biol (Mosk)
PMID:[Construction of partial denaturation charts for DNA with random base distribution]. 3 54

Fluorescamine is a sensitive cytochemical probe for primary amino groups and produces an intense general fluorescence in unfixed tissue sections reflecting the ubiquitous occurrence of such groups. Following treatment with formaldehyde, most primary amino groups react to form derivatives unable to yield fluorescence with fluorescamine. Certain cell systems, however, contain amino groups which do not react with formaldehyde but display strong reactivity with fluorescamine. In formaldehyde- and fluorescamine-treated specimens such cell systems display an intense fluorescence, whereas the majority of tissue constituents are non-fluorescent. Fluorescent cell systems include certain protein- and peptide-secreting cells and a large number of different types of carcinoma cells. In some cases it appears that neoplastic transformation is necessary before the cells display formaldehyde-fluorescamine-induced fluorescence. Available data indicate that the reactive substance(s) are peptide in nature and that the production of such substance(s) may be a general property of carcinoma cells.
Virchows Arch B Cell Pathol Incl Mol Pathol 1979 May 31
PMID:Formaldehyde-fluorescamine-induced fluorescence as a property of carcinoma cells. 3 59

The combined formaldehyde-fluorescamine technique demonstrates fluorescence by mammary carcinoma cells whereas cells of the normal gland or of benign tumours do not show fluorescence. We have studied the correlation between histological staging of the disease, concentrations of oestrogen-binding proteins and the occurrence of formaldehyde-fluorescamine (FF)-inducible fluorescence. Our results demonstrate that the FF-technique detects all types of mammary carcinoma cells irrespective of their concentration of oestrogen receptors. Hence, the FF-technique represents a valuable tool for detecting both hormone-responsive and hormone-unresponsive malignant cells of the mammary gland.
Virchows Arch B Cell Pathol Incl Mol Pathol 1979 Jun 29
PMID:Formaldehyde-fluorescamine-induced fluorescence of mammary carcinoma cells. Lack of concordance with occurrence of oestrogen-binding receptor proteins. 4 8

In order to study the distribution of LH (HCG) receptors on luteal cells ferritin was coupled to ovine LH with glutaraldehyde and purified by gel chromatography. The conjugate (FELH) competed with 125I-hCG for binding to isolated luteal membranes and stimulated a dose-dependent release of progesterone (P) from isolated luteal cells which was inhibited by PGF2 alpha. FELH was distributed as single molecules or in small clusters at intervals on the surfaces of luteal cells labeled at 37 degrees C, 4 degrees C or with formaldehyde prefixation. Capping or preferential labeling at one site was not observed. The general distribution of LH (hCG) binding sites at 37 degrees C was confirmed by light-microscopic autoradiography. The distribution at 4 degrees C or with prefixation was more diffuse than at 37 degrees C suggesting that FELH binding induces small changes in receptor aggregation. Binding of FELH was specific since excess hCG reduced FELH binding to luteal cells. In cells labeled at 4 degrees C, rinsed and warmed to 37 degrees C FELH was observed along cell surfaces and within some coated vesicles and a few lysosomes within minutes suggesting that receptor internalization is a rapid and possibly continual process.
Mol Cell Endocrinol 1979 Aug
PMID:Localization of LH receptors on luteal cells with a ferritin--LH conjugate. 22 60

A method is described which demonstrates the posibility of visualizing protein-complexed DNA by using pronase for both deproteinization and spreading of DNA for electron microscopy. The results show that proteolytic digestion is complete even under conditions of short formaldehyde fixation and pronase is an excellent substitute for cytochrome c for spreading of DNA. The pronase method is successfully applied to virions, native and partially denatured chromatin. The procedure is highly advantageous because it does not require preliminary isolation of DNA, can be applied to microamounts of material and permits the visualization of partial denaturation of DNA in chromatin.
Mol Biol Rep 1979 Aug 31
PMID:The use of pronase for electron microscopy of protein-complexed DNA. 49 60

The formation of glycerol occurs when a solution of DL-glyceraldehyde is heated in the presence of hydrogen sulfide at room temperature. DL-glyceraldehyde and dihydroxyacetone treated with hydrazine, as well as DL-glyceraldehyde incubated with formaldehyde are also partially converted to glycerol. The yields of the above reactions are from approximately 1% to about 3%. The formation of glycerophosphates occurs when glycerol is heated with ammonium dihydrogen phosphate and either urea or cyanamide. The yield of glycerophosphates is about 30%, most of which is sn-glycero-1 (3)-phosphate. These findings indicate that glycerol and sn-glycero-3-phosphate, which are moieties of glycerolipids, could have been formed under conditions which may have prevailed on the primitive Earth.
J Mol Evol 1979 Dec
PMID:Cyanamide mediated synthesis under plausible primitive earth conditions. VI. The synthesis of glycerol and glycerophosphates. 53 3

Even a small amount of formaldehyde is shown to induce a drop in the RNase A enzymatic activity. This drop is rapid from the start and then begins to be slower. A supposition was made on nature of the enzyme activity. Comparison of the effects of formaldehyde on the enzymatic and the destabilizing activity of RNase A was made. The effect of formaldehyde on the enzymatic activity does not correlate with its effect on the ability of RNase to destabilize the DNA double helix.
Mol Biol (Mosk)
PMID:[Effect of formaldehyde on the enzymatic activity of RNAase A]. 56 74

The kinetics and equilibrium of the reaction between nucleic acids components and the products of formaldehyde interaction with ethanolamine and different amino acids has been studied. These parameters were found to be similar for all the products used. The destabilization of the N-glycosidic bond in deoxyadenosine caused by formaldehyde derivatives of different amines was studied. The rate of the cleavage of the N-glycosidic bond under the action of formaldehyde derivatives of glycine and ethanolamine was found to be 10 times greater than that under the action of formaldehyde derivatives of other amines. It is shown that DNA preparations with different content of adenine can be obtained by adding the product of formaldehyde reaction with glycine to DNA.
Mol Biol (Mosk)
PMID:[The action of products of the formaldehyde reaction with different amines on nucleic acids and their components]. 57 Jun 37

It is shown that the kinetics of DNA despiralization in the presence of beta-alanine--formaldehyde reaction product (beta-ALA-FORM) can be described in terms of theory of DNA despiralisation by "slowly reacting agents". Conditions are determined in which beta-ALA-FORM product can be used to establish the concentration of defects in DNA secondary structure. Possible advantages are discussed of using the new agent in the kinetic method of determining DNA defects as compared to formaldehyde, in particular in analysis of DNA complexes with proteins. The data obtained throw some light on the nature of the interaction between beta-alanine and formaldehyde in slightly acidic solutions and with the excess of aminoacid.
Mol Biol (Mosk)
PMID:[Use of the reaction product of beta-alanine and formaldehyde in the kinetic method of determining defects in secondary structure]. 57 55

The rate constants of forward and reverse reactions between methylol derivatives of beta-alanine and deoxycytidine 5'-phosphate, deoxyadenosine 5'phosphate and deoxyguanosine 5'phosphate and the equilibrium constants of these reactions were determined by the spectrophotometric method at 39,5 degrees C and pH 6,95. Besides, the equilibrium constant of the reaction between beta-alanine and formaldehyde was determined. Unlike deoxycytidine and deoxyadenosine 5'-phosphates, interaction of deoxyguanosine 5'phosphate with methylol derivatives is more complicated. A model proposed for the interaction of deoxyguanosine 5'phosphate with methylol derivatives explains the behavior of this nucleotide in the reaction. The kinetic and equilibrium constants of the interaction of methylol derivatives with nucleotides investigated exceed by two or three orders of magnitude the corresponding constants of the interaction of formaldehyde with these nucleotides.
Mol Biol (Mosk)
PMID:[Kinetics and equilibrium of reactions between nucleotides and methylol derivatives of beta-alanine]. 74 10


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