Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Human liver alpha-L-fucosidase was purified to apparent homogeneity and analyzed for carbohydrate content primarily by gas-liquid chromatography (glc). The enzyme is about 7% carbohydrate by weight and contains the following sugars (residues per 50, 000 molecular weight subunit): mannose (8.3), glucosamine (4.3) (presumably N-acetylated), sialic acid (1.6) and glucose (1.6). Galactose (0.8) and L-fucose (1.8) were also found but their presence may be due to artifacts of the purification procedure.
Mol Cell Biochem 1977 Nov 25
PMID:Carbohydrate composition of purified human liver alpha-L-fucosidase. 60 Feb 69

1. The limitations inherent in the conventional treatment of glucose decay curves as first-order rate systems are described. 2. The conventionally derived K value is a rate constant and should not be confused with a rate. 3. First-order systems are described by this rate constant and the initial concentration of substance studied. They cannot be described by either factor alone. 4. Two parallel curves cannot both result from first-order systems. 5. If K is conventionally calculated for two parallel curves, then the value obtained for the upper curve must be smaller than the value for the lower.
Clin Sci Mol Med 1977 Jan
PMID:The analysis of decay curves. 60 68

1. Galactose utilization after intravenous injection was measured in fed and fasted man together with changes in blood glucose, lactate and insulin. 2. Feeding did not alter blood galactose half-life. 3. The mean increases in blood glucose and lactate were greater in the fasted subjects but their concentrations reached similar values in both fed and fasted states. 4. Plasma insulin increased after galactose in the fasted state, but there was no change in the fed state, indicating that galactose is not insulinogenic. 5. After an intravenous galactose load in the fed state insulin appears to inhibit hepatic glucose release. 6. An intravenous galactose test might be a useful measure of hepatic glucose release under different physiological and pathological conditions.
Clin Sci Mol Med 1978 Jan
PMID:The metabolic response to galactose as a measure of hepatic glucose release in man. 62 Apr 87

1. Glucose absorption, water absorption and dipeptide hydrolase activities have been determined in isolated rat small intestine at 1, 3, 5 and 21 days after a single intraperitoneal injection of 5-fluorouracil. 2. Absorption rates and enzyme activities were elevated 1 day after treatment, but were reduced to 40% of control values at 3 and 5 days. Changes were seen regardless of whether absorption was expressed per unit length or per unit dry weight of intestine. 3. There were highly significant positive correlations between glucose or water absorption rates and peptidase activities, especially in proximal jejunum. The most significant correlation was observed between water absorption rate and jejunal L-Leu-Gly hydrolase activity. 4. Malabsorption may account for some of the gastrointestinal side effects associated with treatment with 5-fluorouracil. Enzyme measurements may be useful as an index of intestinal function.
Clin Sci Mol Med 1978 Apr
PMID:Changes in absorptive and peptide hydrolase activities in rat small intestine after administration of 5-fluorouracil. 63 72

1. The effects of streptozotocin-diabetes on the local and general responses to a 4 h period of bilateral hind-limb ischaemia in the rat have been investigated. The rats were injured 48 h after the intravenous injection of the streptozotocin. 2. Less fluid was lost from the circulation into the injured limbs after injury in the diabetic rats and this was directly related to the severity of the diabetes, but could not be explained by dehydration. However, when the diabetic and nondiabetic injured rats were considered together there was significant negative correlation between either plasma osmolality or plasma glucose concentration and water content in the injured hind limb. 3. The relationship between plasma glucose concentration and plasma osmolality was changed by injury such that, particularly in the injured diabetic rats, plasma osmolality at a given glucose concentration was higher than that predicted from the relationship betweeen these variables in the uninjured rat.
Clin Sci Mol Med 1978 Apr
PMID:Effect of streptozotocin-diabetes on the local and general responses to injury in the rat. 63 75

1. Hepatic carbohydrate metabolism was studied by an intravenous galactose test in control patients, malnourished non-septic patients, patients with prolonged severe sepsis and patients after recovery from sepsis. 2. Blood galactose half-life was not significantly increased in the septic group despite abnormal liver-function tests, whereas it was approximately doubled in the malnourished patients. 3. The rise in blood glucose after galactose injection was less in both the septic and malnourished groups, as compared with that in the control subjects. 4. Fasting blood glucose, lactate and pyruvate concentrations were similar in all groups, whereas blood ketone bodies were increased in the malnourished and septic groups, and blood alanine was decreased only in the septic group. 5. The changes in hepatic metabolism and function were reversible on recovery from sepsis. 6. It is suggested that alterations in hepatic blood flow and the metabolic fate of galactose within the liver may explain the changes in the metabolic response to galactose observed in malnourished or septic patients.
Clin Sci Mol Med 1978 Aug
PMID:Galactose and hepatic metabolism in malnutrition and sepsis in man. 67 28

A study has been made of urinary peptide output in rats before and after production of a Fanconi syndrome induced by a single injection of sodium maleate. There was an unequivocal increase of urinary peptides on the first and second days after the injection, without any detectable change in the concentration of plasma peptides. 2. Similar results were obtained in osteolathyritic rats in which skeletal lesions had been produced by ingestion of beta-aminopropionitrile. 3. The fractional amino acid content of urinary peptides after maleate and beta-aminopropionitrile is shown to be significantly different from that in control animals. 4. Evidence is presented that the increased output of peptides is mainly due to increased renal clearance similar to that previously described for amino acids, glucose and several electrolytes in this type of experimental Fanconi syndrome.
Clin Sci Mol Med 1978 Aug
PMID:Peptide excretion in experimental Fanconi syndrome in the rat. 67 29

The interaction of arginine vasotocin (AVT) and norepinephrine (NE) upon pineal gland indoleamine synthesis was investigated. Rat pineal glands were incubated for 10 h in Krebs--Ringer bicarbonate plus 2 mg/ml glucose, 1 mg/ml bovine serum albumin, [14C]tryptophan, NE (10(-6) M), and log doses of AVT ranging from 100 ng to 10 microgram. Incubation media were extracted for [14C]serotonin while the other [14C]indoleamines, melatonin, hydroxyindoleacetic acid (HIAA), methoxyindoleacetic acid (MIAA), N-acetylserotonin (NAS), hydroxytryptophol (HTOL), and methoxytryptophol (MTOH) were separated by thin-layer chromatography. Serotonin metabolism was decreased by 0.1 microgram AVT and NAS decreased by 1.0 microgram AVT. Melatonin synthesis was decreased by both 0.1 and 1.0 microgram AVT. AVT also decreased the conversion of [14C]serotonin to MIAA and to HTOL. The data indicates that AVT decreased NE-stimulated pineal indoleamine synthesis in vitro and further suggests that AVT may participate in the intracellular regulation of melatonin synthesis.
Mol Cell Endocrinol 1978 Jun
PMID:Interaction of arginine vasotocin and norepinephrine upon pineal indoleamine synthesis in vitro. 68 Mar 35

1. Infusion of a triglyceride emulsion (Intralipid) into overnight fasted normal subjects produced a rise in plasma free fatty acids (FFA) and blood ketones. 2. Glucose given orally 60 min after the start of the Intralipid infusion produced a sharp fall in blood ketones without much change in plasma FFA. 3. An infusion of glucagon given together with Intralipid did not alter the reduction in blood ketones produced by oral glucose in normal subjects. 4. Oral glucose given 60 min after the start of the Intralipid infusion in three insulin-requiring diabetic subjects produced no fall in blood ketones. 5. The results suggest that glucose prevents the increase in blood ketones after Intralipid through an increase in insulin secretion rather than through a suppression of glucagon or as a direct effect of glucose. 6. It is most likely that the effect of insulin is to inhibit hepatic ketogenesis.
Clin Sci Mol Med 1978 Nov
PMID:Evidence for an hepatic anti-ketogenic effect of insulin in man. 72 4

Gluconeogenesis by isolated hepatocytes resulted in glucose release but insignificant rates of glycogen synthesis. The effectiveness of precursors was similar for hepatocytes from fed and starved chickens except for impaired gluconeogenesis from pyruvate when compared to lactate in lactate starved chicken hepatocytes. The impairment was caused by limitations in cytosolic NADH production as a result of the mitochondrial location of phosphoenolpyruvate carboxykinase in chicken liver. The order of effectiveness of precursors on hepatic gluconeogenesis was generally similar to the effects of precursors on increasing the plasma glucose concentration in vivo. The exceptions were caused by interactions with other precursors in vivo. The alteration of the NADH/NAD+ ratio by ethanol and ATP/ADP ratio by adenosine could play significant roles in the control of precursor conversion to glucose. Physiological glucagon concentrations stimulated gluconeogenesis from precursors entering the pathway both above and below the level of triose phosphates, and its effect were mimicked by dibutyryl cyclic AMP. Previous results on the effects of precursor and glucagon injection on the plasma glucose concentration of chickens in vivo can largely be explained by effects at the hepatic level. Isolated chicken and rat hepatocytes share many common features. Qualitatively the ordering of gluconeogenic effectiveness was similar but quantitive differences existed as a result of differing activities and cellular locations of enzymes. Neither preparation readily synthesised glycogen and the sensitivity to glucagon was similar.
Mol Cell Biochem 1978 Dec 22
PMID:Hepatic gluconeogenesis in chickens. 74 98


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