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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The stability of mRNA has been measured in 3T3 cells in the resting and the growing states, and also during the transition from the resting to the growing state. Pulse labled poly (A)+ mRNA chased with uridine and cytidine supplemented growth medium decayed with a half-life of 6.5 hr in the resting state, 26 hr during the transition from the resting to the growing condition, and 18 hr during serum-stimulated growth. The half-life of poly(A)+ mRNA determined by steady state labeling yielded similar results in resting and serum-stimulated 3T3 cells. Thus during the transition from resting to serum-stimulated growth in 3T3 cells poly(A)+ mRNA becomes more stable.
Mol Biol Rep 1977 Dec
PMID:Stabilization of mRNA following serum-induction of quiescent 3T3 cells. 59 72

The intracellular influenza virus-containing structures involved in RNA synthesis in the cytoplasm and in the nucleoplasm of infected chicken fibroblasts were studied. Two approaches were used: (1) short pulse labeling of infected cell with [3H]uridine; (2) determination in vitro of polymerase activity of intracellular virus-specific structures. Both methods revealed functionally active virus-specific structures in the nucleoplasm and showed that a functionally active virus-specific structure was localized in the nucleoplasm of infected cells. This structure contained proteins of the viral ribonucleoprotein, but sedimented somewhat faster (at 60--90S in velocity sucrose and glycerol gradients). Meanwhile, polymerase-containing structures in the cytoplasm of infected cells sedimented in the position of viral ribonucleoproteins (25--60S).
Mol Biol (Mosk)
PMID:[Intracellular structures of influenza virus]. 65 75

A mechanism responsible for proteolytic deproteinization of influenza virus A2 Hong-Kong (I)68 by plasmatic membranes of sensitive cells was studied. Presence of trypsinlike protease in plasmatic membranes of white mice lungs was demonstrated. A considerable inhibition of the membrane proteolitic activity was obtained in the presence of epsilon-aminocaproic acid. Disintegration of the virus labeled by [3H]uridine by plasmatic membranes was investigated and it was found that this process required ATP. Inhibition of the protease activity by epsilon-aminocaproic acid led to the suppression of deproteinization of influenza virus. The experimental data obtained indicate that the proteolytic enzymes of plasmatic membranes participate in the complex process of virus "uncoating".
Mol Biol (Mosk)
PMID:[Proteolytic mechanism of deproteinization of influenza virus by plasmatic membranes]. 75 90

Various structural analogues of cytosine and uracil nucleosides were tested as potential inducers of the nucleoside catabolizing (cyt) enzymes in Salmonella typhimurium. Some analogues, e.g. 5'-O-alkyl cytidines and uridines, resistant to catabolic enzymes, were as effective as the natural inducers cytidine and uridine; but etherification of one of the cis 2' or 3'hydroxyls fully abolished activity, pointing to a requirement of an intact ribose cis-glycol system for activity. A uridine analogue in the syn conformation, 6-methyluridine, a good substrate for uridine phosphorylase, was inactive as an inducer. The behavior of various other analogues, in relation to their structure, conformation and substrate properties, indicated the absence of any correlation between inducing activity and substrate susceptibility. The overall findings are consistent with conclusions derived from genetic experiments. The active analogues apparently act via similar pathways, and probably affect the same regulatory mechanism(s) as the natural inducers.
Mol Biol Rep 1975 Dec
PMID:Pyrimidine nucleoside analogues as inducers of pyrimidine nucleoside catabolizing enzymes in Salmonella typhimurium. 76 67

1. New high molecular weight RNA species have been found in an RNase III deficient mutant of E. coli. These RNA's were very minor but stable components of the cells, and their molecular weights, which range from 3-5.5 million daltons, are higher than that of 30S precursor ribosomal RNA. In these respects these RNAs are similar to the 2.5 M RNA reported previously (Yuki and Wittmann, 1974). 2. A method to analyse minor RNA components is described. A linear relationship between logarithms of molecular weights and logarithms of distance moved in 1.5-7.5% polyacrylamide concentration gradient gels is also described in this report. 3. DNA species whose molecular weights ranged from 1.8 to 5.5 million daltons and also a species of 8 million daltons are described. two techniques commonly used to identify RNA, viz. DNase treatment and labeling with radioactive uridine, are discussed in connection with these DNAs. 4. The determination of the molecular weight of 30S precursor ribosomal RNA is discussed and it is suggested that this RNA is heterogenous, consisting of two species of molecular weight 1.8 million daltons and 2.0 million daltons, respectively.
Mol Gen Genet 1976 Mar 22
PMID:Detection of ribonucleic acids which are larger than 30S precursor ribosomal RNA in RNase III deficient E. coli cells. 77 88

Two allelic auxotrophic mutants at a locus close to the bw locus (2-104.5) of Drosophila melanogaster are described. The mutants respond to dietary ribonucleosides (uridine, cytidine, adenosine, guanosine and inosine) but less well to bases or pyrimidine precursors. This phenotype is unique to these mutants. We suggest that the mutants are defective in phosphoribosyl pyrophosphate biosynthesis.
Mol Gen Genet 1976 Aug 10
PMID:Nucleoside auxotrophy in Drosophila: an autosomal locus yielding mutants supplementable by purine and pyrimidine ribonucleosides. 82 79

Exponential growing Tetrahymena pyriformis organisms were labelled with (3H) uridine or (3H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion. Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.
J Mol Evol 1976 Aug 03
PMID:Fractionation of RNA from tetahymena by affinity chromatography on poly-U-Sepharose. 82 41

In MCF-7 human breast cancer cells, insulin stimulated the rate of [3H]uridine incorporation into RNA, [3H]thymidine incorporation into DNA, and [3H]leucine incorporation into protein. In addition, hydrocortisone appeared to augment the effect of insulin, by further increasing the rate of [3H]uridine incorporation into RNA and [3H]thymidine incorporation into DNA. A significant increase in the total amount of DNA and protein was present in cultures treated with insulin compared to untreated controls. Hydrocortisone was shown to augment the insulin effect on total protein accumulation and total RNA accumulation in MCF-7 cells.
Mol Cell Endocrinol 1977 Jun
PMID:Hydrocortisone enhancement of insulin's action on macromolecular synthesis in MCF-7 cells. 88 87

Chicken embryos were pulse-labelled in vivo with [3H]uridine (10 min), the chromatin isolated and treated with DNAse I. The residual chromatin was separated from the degradation products by centrifugation. The nascent pulse-labelled RNA is completely recovered in the residual chromatin even after prolonged incubation with DNAase I, whereas the DNA is completely degraded to 80 base polynucleotide fragments and smaller fragments.
Mol Biol Rep 1977 Jun
PMID:Template active chromatin structures: degradation by deoxyribonuclease I. 88 97

In vitro RNA synthesis has been analyzed using autoradiographic and electrophoretic methods in isolated 1-2 mm segments of rat seminiferous tubules. The cellular composition of each segment was accurately identified using microscopic analysis of the transillumination pattern of the freshly isolated, unstained, seminiferous tubules combined with phase-contrast microscopy of the living spermatogenic cells. The RNA synthesized in the seminiferous tubules was found to be mostly heterogenous nuclear RNA (HnRNA), which appeared to have a long lifetime. It was most actively formed in the stages which contain mid-pachytene spermatocytes. Formation of rRNA was slow in all stages and it was first observed when a 2-h pulse with [3H]uridine was followed by a 6-h chase. A very low RNA synthetic rate was observed in the stage containing the meiotic reduction divisions. The function of the meiotic RNA in regulation of spermiogenesis is discussed.
Mol Cell Endocrinol
PMID:RNA synthesis in different stages of rat seminiferous epithelial cycle. 95 50


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