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Query: UNIPROT:P06889 (Mol)
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A DNA fragment corresponding to a low-temperature- and ABA-responsive gene (Scdhn1) was amplified by PCR from genomic DNA of a wild, frost-resistant potato species, Solanum commersonii. A homologous gene (Stdhn1) was identified in Solanum tuberosum cv. Bintje, a frost-sensitive domesticated potato cultivar. The expression of the gene was studied during low temperature and ABA treatments in both Solanum species. The analysis revealed that both low temperature and ABA lead to the accumulation of a 1 kb transcript that corresponded to the PCR fragment. The induction of the gene was relatively rapid and maximum amounts of the transcripts were detected already after 1 day and 7 h of treatment with low temperature and ABA, respectively. Previous results have shown that there is no increase in the amount of endogenous ABA in S. tuberosum during low-temperature treatment, which indicates that two independent signalling pathways lead to the induction of this gene.
Plant Mol Biol 1996 Jan
PMID:Induction of homologous low temperature and ABA-responsive genes in frost resistant (Solanum commersonii) and frost-sensitive (Solanum tuberosum cv. Bintje) potato species. 861 56

In Craterostigma plantagineum the CDeT-6-19 and CDeT-27-45 genes are expressed following desiccation and/or ABA treatment. Their promoters were fused to the beta-glucuronidase reporter gene (GUS) and tested in transgenic Arabidopsis. GUS activity was measured in mature Arabidopsis seeds, and the responsiveness to ABA in vegetative tissue was found to be limited to the early developmental stages. When transgenic plants were crossed with plants over-expressing the ABI3 gene, it was observed that ABI3 is not required for ABA induction of the CDeT-6-19 promoter, whereas it is crucial for expression of the CDeT-27-45 promoter.
Plant Mol Biol 1996 Jan
PMID:Differential regulation of two ABA-inducible genes from Craterostigma plantagineum in transgenic Arabidopsis plants. 861 58

We have characterized the structure and expression of a senescence-associated gene (sen1) of Arabidopsis thaliana. The protein-coding region of the gene consists of 5 exons encoding 182 amino acids. The encoded peptide shows noticeable similarity to the bacterial sulfide dehydrogenase and 81% identity to the peptide encoded by the radish din1 gene. The 5'-upstream region contains sequence motifs resembling the heat-shock- and ABA-responsive elements and the TCA motif conserved among stress-inducible genes. Examination of the expression patterns of the sen1 gene under various senescing conditions along with measurements of photochemical efficiency and of chlorophyll content revealed that the sen1 gene expression is associated with Arabidopsis leaf senescence. During the normal growth phase, the gene is strongly induced in leaves at 25 days after germination when inflorescence stems are 2-3 cm high, and then the mRNA level is maintained at a comparable level in naturally senescing leaves. In addition, dark-induced senescence of detached leaves or of leaves in planta resulted in a high-level induction of the gene. Expression of the sen1 gene was also strongly induced in leaves subjected to senescence by 0.1mM abscisic acid or 1 mM ethephon treatment. The induced expression of the gene by dark treatment was not significantly repressed by treatment with 0.1 mM cytokinin or 50 mM CaCl2 which delayed loss of chlorophyll but not that of photochemical efficiency.
Plant Mol Biol 1996 Feb
PMID:A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescence. 862 6

We have isolated and sequenced two cDNA clones (PM 18.2A; PM 18.2B) from Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) which encode for the low-molecular-weight heat shock proteins (LMW HSPs) of 18.2 kDa. The predicted amino acid sequences of the two Douglas fir proteins are 97.5% identical. A phylogenetic tree of class I LMW HSPs showed that the PM LMW HSPs are found within a subgroup consisting exclusively of dicot species indicating that class I LMW HSPs evolved from a common ancestor predating the divergence of gymnosperms and angiosperms. Northern blots of RNA from dry, imbibed, stratified and germinated seeds revealed a notable induction of LMW HSP transcripts during post-germination and early seedling growth. Unlike previous reports, the expression of these HSPs appears to be primarily restricted to seedlings as mRNA transcripts were detected at very low levels during seed development and desiccation. Maximum induction of LMW HSPs in seedlings occurred during heat shock treatment at 38-40 degrees C, whereas cold shock or wounding failed to induce HSP transcripts. The transcription of HSP genes is up regulated by GA, MeJA and auxin and is down regulated by ABA. Methyl jasmonate treatment induced expression of these genes in dormant seeds of Douglas fir. The expression of class I cytoplasmic LMW HSPs in seedlings and their regulation by plant growth regulators suggests specific roles in plant development other than desiccation tolerance.
Plant Mol Biol 1996 Mar
PMID:Post-termination-induced and hormonally dependent expression of low-molecular-weight heat shock protein genes in Douglas fir. 870 23

A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings, whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
Plant Mol Biol 1996 Mar
PMID:cDNA encoding a wheat (Triticum aestivum cv. Chinese spring) glycine-rich RNA-binding protein. 870 37

DC8 is a late embryogenesis-abundant (LEA) protein gene isolated from carrot (Daucus carota). Deletion analysis of the DC8 promoter was performed to determine the sequences required for ABA and seed-specific regulation of DC8 transcription. To investigate the mechanism of DC8 expression during seed development, chimeric gene constructs containing DC8 promoter fragments fused to a promoterless beta-glucuronidase gene (DC8:GUS) were introduced into carrot, tobacco (Nicotiana tobacum) and Arabidopsis thaliana plants. Seed-specific DC8 expression patterns was conserved among the three plant species. However, differences among the species in the patterns of DC8 expression in the embryo and endosperm that correlated with differences in the rates of embryo and endosperm growth were found. Lack of correspondence between DC8 activation and embryo development among the seeds of the three species suggests that DC8 expression, which is associated with seed maturation, is not coupled to the embryo development program. The presence of DC8 activity in carrot callus and endosperm is consistent with the notion that DC8 expression is independent of embryo morphogenesis. A similar DC8 activity time-course during callus induction and seed development suggests that explantation and 2,4-D treatment initiates a course of events similar to that in the carrot ovule. After fertilization, two pathways one leading to embryo development and another to seed maturation are initiated, but they are not closely linked. As a result we find DC8, part of the maturation program, being activated at different embryonic stages in different plant species.
Plant Mol Biol 1996 Apr
PMID:Expression of DC8 is associated with, but not dependent on embryogenesis. 870 45

The tobacco anionic peroxidase gene encodes the predominant peroxidase isoenzyme in the aerial portions of tobacco. Three kb of the peroxidase promoter was joined to the coding region of the Escherichia coli beta-glucuronidase gene (GUS), and transiently expressed in tobacco mesophyll protoplasts in the presence or absence of plant growth regulators. Benzyladenine, ethylene, and gibberellic acid did not affect peroxidase gene expression. Abscisic acid slightly inhibited expression at high concentrations. The auxins indole-3-acetic acid (IAA) and naphthaleneacetic acid strongly suppressed peroxidase expression. We observed half maximal suppression at 30 microM IAA. An anti-auxin, p-chlorophenoxyisobutyric acid (PCIB), enhanced expression from the peroxidase promoter above that of untreated controls or restored activity when used in combination with IAA. Sequencing 3 kb of the peroxidase promoter revealed many potential regulatory elements based on sequence homology to previously characterized genes. This includes several consensus transcription factor binding sites found in auxin-regulated promoters. 5' deletions of the peroxidase promoter/GUS fusion revealed several positive and negative regulatory elements. An upstream enhancer element was found between -3146 and -638 from the start of transcription. A strong silencer element was observed between -638 and -220. Removal of this silencer resulted in a truncated promoter (-220) with 100% activity of the full-length promoter (-3146). Inhibition by auxin was observed with all 5' deletions.
Plant Mol Biol 1996 Jun
PMID:Phytohormone control of the tobacco anionic peroxidase promoter. 879 Feb 89

We have isolated a gene and cDNA from Brassica napus encoding a hybrid-proline-rich protein. The putative protein is modular in structure. The N-terminal domain has properties of a signal peptide which would direct the protein into the ER. Amino acids 27 to 287 comprise three domains which contain high levels of proline and several other amino acids common in proline-rich cell wall proteins. These domains are characterised by repeating amino acid motifs. The C-terminal domain (amino acids 288 to 376) contains three putative membrane-spanning regions and shows a high degree of amino acid similarity to known hybrid-proline-rich proteins from several species. It is likely that the protein is secreted from the cell, located in the cell wall and anchored in the plasma membrane via the C-terminal domain. Transcripts encoding this protein are induced in leaf tissue within 8 h of cold treatment and decrease rapidly when plants are returned to normal temperatures. The transcripts are not induced by heat shock, dehydration, exogenous ABA or wounding, whereas transcripts of a control B. napus gene are induced by dehydration and ABA. The possible function of this protein in cold tolerance is discussed.
Plant Mol Biol 1996 Jul
PMID:Transcripts of a gene encoding a putative cell wall-plasma membrane linker protein are specifically cold-induced in Brassica napus. 880 8

We have studied two lines of sunflower (Helianthus annuus L.) selected in the field as drought-tolerant (R1 genotype) or drought-sensitive (S1 genotype). When subjected to drought conditions, the R1 line was able to maintain high leaf water potential longer and wilted later than the S1 line. Therefore, this indicates that R1 tolerance includes a leaf-adaptive response. By subtractive hybridization, we have isolated six different cDNAs (designated sdi for sunflower drought-induced) corresponding to transcripts accumulated in R1 and S1 leaves during adaptive response. Analysis of transcript accumulation in response to drought in both genotypes suggests a preferential expression of three sdi genes in the tolerant line. Abscisic acid-mediated induction, analysed in R1 leaves, was observed for only four sdi genes. Sequence analysis of six sdi clones revealed that five clones were related to known proteins including non specific lipid transfer proteins (nsLTP), early light-induced proteins (ELIP), l-aminocyclopropane-l-carboxylate oxidase (ACC oxidase) or dehydrins, predicted to be involved in a wide range of physiological processes.
Plant Mol Biol 1996 Jul
PMID:Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype. 880 12

A cDNA and two genomic clones comprising highly similar genes that encode a protein with a Myb-related DNA-binding domain were isolated from the resurrection plant Craterostigma plantagineum. The structure of cpm5 and cpm10 (Craterostigma plantagineum myb) genes consists of three putative exons encoding a protein of 36.6 kDa. The cDNA of cpm7 encodes a closely related protein of 36.8 kDa. The canonical Myb domain present in transcriptional activators of yeast, animals and plants was localized in the amino terminus of deduced Cpm5, Cpm7 and Cpm10 proteins and corresponds to the two Myb repeats found in plants. The Myb domain of Cpm deduced proteins and a short stretch of amino acids adjacent to this region are closely related to a myb gene from Arabidopsis thaliana which is expressed in response to osmotic stress and ABA. The rest of the deduced protein has no similarity to other reported sequences. The myb-related genes in the Craterostigma genome comprise a small gene family of 6-8 members as estimated by hybridization with a bona fide Myb domain probe. Northern blot experiments showed specific expression of cpm10 in undifferentiated callus tissue up-modulated by ABA and expression of cpm7 mRNA in roots up-regulated by dehydration.
Plant Mol Biol 1996 Nov
PMID:A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation. 898 May 22


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