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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids or S. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared.
Mol Biol Rep 1979 Feb 15
PMID:Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA. 10 33

The formation of mating pairs between F- and Hfr cells resulted in increased sensitivity of recipient deoxyribonucleic acid (DNA) to single-strand-specific S1 nuclease, from 3.6% to 23.5% after 30 min conjugation. A comparable amount of single strand regions in the DNA of mated wild type and recA mutant cells was detected. 10 min of conjugation resulted in almost the same amount of single-strand recipient DNA as 30 min of continuous transfer of donor DNA. Also the transfer of plasmid DNA from F+ recA strain led to the occurrence of single-strand recipient DNA. In similar experiments with Hfr tra mutant no such effect was observed. We conclude that alterations in the sechases of conjugation associated with the formation of mating pairs and/or initiation of transfer donor DNA.
Mol Gen Genet 1977 Mar 16
PMID:Mechanism of conjugation and recombination in bacteria XVI: single-stranded regions in recipient deoxyribonucleic acid during conjugation in Escherichia coli K-12. 32 77

Major kinetic parameters of endonuclease S1 were determined on superhelical bacteriophage PM2 DNA and on relaxed nicked circular PM2 DNA. At 37 degrees and 0,25 M NaCl, the Michaelis constants were respectively 1.7 . 10(-8) M and 1 . 10(-9) M, and catalytic constants were respectively 0.36 sec-1 and 1.2 . 10(-2) sec-1. The inhibition of the enzyme reaction by its product was detected.
Mol Biol (Mosk)
PMID:[Kinetic parameters of superhelical DNA cleavage by endonuclease S1]. 50 58

A purification method for isolating homogeneous single-strand specific nuclease S1 from alpha-amyloryzin has been developed. The yield was about 16% and purification factor--9000. Nuclease S1 thus obtained was proved to be free of contaminations of any others nucleolytic enzymes. It is shown for the first time that ribo- and deoxy-dinucleosidemonophosphates are hydrolyzed by nuclease S1 to form 5'-nucleotides with pH optimum for ApA equal to 4.6.
Mol Biol (Mosk)
PMID:[Isolation and some properties of homogenous nuclease S1 from alpha-amyloryzin]. 54 81

The slow reassociating fraction of mouse DNA ("unique DNA"), when allowed to reassociate in 0.14 M sodoum phosphate buffer at 50 degrees C showed a biphasic melting curve with a transition at 78--80 degrees C. On the basis of this feature, the slow reassociating DNA was separated preparatively into two fractions: "unique DNA" I and II. Their duplexes showed differences with respect to thermal stability, S1 nuclease resistance and rate of reassociation. About one third of the sequences in each fraction were fraction-specific. The conclusion was drawn that for "unique DNA" I these should be the low repetitive or single copy related sequences (multigene families) and for "unique DNA" II--the unrelated single copy sequences or recent families of low repetitive not yet diverged sequences.
Mol Cell Biochem 1978 Jun 28
PMID:On the heterogeneity of the slow reassociating ("unique") DNA. 67 5

Arrangement of repetitive and single copy DNA sequences in the DNA of 8 Echinodermata species (sea urchins, starfishes and sea-cucumber) has been studied. Comparison of the reassociation kinetics of short and long DNA fragments assayed by hydroxyapatite binding indicates that the pattern of DNA sequence organization of all these species is similar to the so called Xenopus pattern found in genomes of most animals and plants. Interspecies differences consist mainly in the quantities of sequences of various repetition degrees and their interspersion with each other and with single copy sequences. Measurements of the size of S1 nuclease resistant reassociated repetitive sequences show variability in the relative quantities of long and short repetitive sequences of different species. Difference in the arrangement of single copy and repetitive sequences between Echinodermata species are not related to their evolutionary proximity.
Mol Biol (Mosk)
PMID:[Single copy and repetitive DNA sequences of Echinodermata. I. Arrangement of DNA sequences of different multiplicity]. 74 4

DNA of the IS-elements IS1 and IS2 was prepared by digestion of appropriate heteroduplex molecules with endonuclease S1, followed by sucrose gradient centrifugation or gel electrophoresis. The material obtained is homogeneous with regard to size. The length of IS1 DNA is 820 +/- 65 nucleotides, the length of IS2 DNA is 1,350 +/- 70 nucleotides. IS1 DNA is not cleaved by the restriction endonucleases Eco R1, Hind II or Hind III. IS2 DNA is cleaved once by each of the two latter enzymes. The buoyand density determined by equilibrium centrifugation of Hg-complexes in Cs2so4 corresponds to a GC content of approximately 50%. Labelling with polynucleotide kinase indicates that both IS DNA's have a guanosyl residue at both of their 5'-termini.
Mol Gen Genet 1976 May 07
PMID:The isolation of IS1 and IS2 DNA. 93 52

About 5% of the loach (Misgurnus fossillis L.) DNA reassociates at Cot values virtually equal to zero. 50% of the reassociate are resistant to nuclease S1 treatment and reveal the properties of double-stranded structure when chromatographed on hydroxyapatite. Some proofs of palindromic (hair-pin) nature of this fraction have been obtained. An introduction of nicked scissions into the palindromic DNA by pancreatic DNAse treatment under pessimal conditions made it possible to investigated reassociation kinetics of the nucleotide sequences forming palindromes. Two different types of nucleotide sequences appeared to exist in the palindromic fraction with repetition frequencies (ni) equal to 3 X 10(2) and about 1. Homologies were revealed between these sequences and the fraction of corresponding repetition frequency of the main part of the genome. Adjacent sequences contain repetitive regions with ni equal to 10(5) and 5 X 10(3). On the basis of the data obtained some conclusions were made about the distribution of usual and inverted repetitions in the loach genome.
Mol Biol (Mosk)
PMID:[Detection and several characteristics of leach genome palindromes]. 94 May 59

The organization of repetitive and single copy DNA sequences in sea urchin DNA has been examined with the single strand specific nuclease S1 from Aspergillus. Conditions and levels of enzyme were established so that single strand DNA was effectively digested while reassociated divergent repetitive duplexes remained enzyme resistant. About 25% of sea urchin DNA reassociates with repetitive kinetics to form S1 resistant duplexes of two distinct size classes derived from long and short repetitive sequences in the sea urchin genome. Fragments 2,000 nucleotides long were reassociated to Cot 20 and subjected to controlled digestion with S1 nuclease. About half of the resistant duplexes (13% of the DNA) are short, with a mode size of about 300 nucleotide pairs. This class exhibits significant sequence divergence, and principally consists of repetitive sequences which were interspersed with single copy sequences. About one-third of the long duplexes (4% of the DNA) are reduced in size after extensive S1 nuclease digestion to about 300 nucleotide pairs. About two-thirds of the long resistant duplexes (8% of the DNA) remains long after extensive SI nuclease digestion. These long reassociated duplexes are precisely base paired. The short duplexes are imprecisely paired with a melting temperature about 9 degrees C below that of precisely paired duplexes of the same length. The relationship between length of repetitive duplex and precision of repetition is confirmed by an independent method and has been observed in the DNA of a number of species over a wide phylogenetic area.
J Mol Evol 1976 Dec 31
PMID:Evolutionary divergence and length of repetitive sequences in sea urchin DNA. 101 29

About 2% of mouse DNA reassociates at extremely low Cot values (10-7-10-6 mole-liter-1-sec). This DNA fraction was isolated with the aid of nuclease S1 and purified by chromatography on hydroxyapatite. The study of this fraction suggests that it has a structure of hairpin type. The DNA of the hairpins can hybridize with sequences forming the double-stranded regions in pre-mRNA. Probably at least some of the DNA of the hairpins, described as "reversed repeating sequences" of DNA, is transcribed with the formation of structures of hairpin type in pre-mRNA.
Mol Biol 1975 Jan
PMID:Structure of nuclear pre-mRNA. VI. "Reversed repeats" in animal DNA and their hybridization with double-stranded regions of pre-mRNA. 116 48


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