Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of nitecapone (3[(3,4-dihydroxy-5-nitrophenyl) methylene]-2,4-pentanedione) on lipid peroxidation in guinea pig liver microsomes was assessed with the thiobarbituric acid assay. Nitecapone inhibited dose-dependently hydroxyl and peroxyl radical induced lipid peroxidation with IC50-values of 11.1 microM and 16.2 microM, respectively. Nitecapone was an effective antioxidant in native microsomes, where it potentiated glutathione-dependent protection against oxidative stress. Nitecapone provided dose-dependent reduction in lipid peroxidation lasting up to three hours in the presence of glutathione. The long-lasting effect was lost, when the microsomes were boiled. These data suggest that nitecapone is an effective scavenger of oxygen derived free radicals, and that its antioxidant properties are potentiated by glutathione.
Biochem Mol Biol Int 1995 Feb
PMID:Antioxidant properties of nitecapone are potentiated by glutathione. 766 94

Nitecapone [3-(3,4-dihydroxy-5-nitrobenzylidene)-2,4-pentanedione], is a scavenger of nitric oxide produced in vitro. It reduced the rate of methemoglobin formation from oxyhemoglobin exposed to nitric oxide generated from the reaction of hydroxylamine with Complex I of catalase and it decreased the amount of nitrite formed in the reaction of oxygen with nitric oxide generated from sodium nitroprusside. Nitecapone also affected the L-arginine dependent accumulation of nitrite in a suspension of peritoneal rat neutrophils. The related compounds entacapone [2-cyano-N, N-diethyl-3-(3,4 dihydroxy-5-nitrobenzyl)-propenamide] and OR 1246 [3-(3,4-dihydroxy-5-nitrobenzyl)-2,4-pentanedione] were also able to scavenge nitric oxide. The action of nitecapone on nitric oxide expands the role of nitecapone as a scavenger of reactive oxygen species, and suggests nitecapone, entacapone and OR 1246 as potential therapeutic agents for the treatment of diseases connected with increased production of nitric oxide.
Biochem Mol Biol Int 1994 Oct
PMID:Nitecapone: a nitric oxide radical scavenger. 783 30

Therapeutic agents which target NF-kappa B transcription factor may be useful in the management of AIDS, cancer and inflammation. Since oxidative stress has been implicated in the signaling pathway, the use of antioxidants to inhibit NF-kappa B activation has gained attention. In the present study, we examined the effects of catechol derivatives, nitecapone and OR-1246, which have been identified to possess potent antioxidant properties, on NF-kappa B activation by monitoring its DNA binding activity. Both nitecapone and OR-1246 (10-300 microM) inhibited NF-kappa B activation induced by tumor necrosis factor-alpha in Jurkat T (acute human leukemia) cells. Nitecapone was a better inhibitor than OR-1246. The observed effects may, at least in part, be due to the ability of the two compounds to directly inhibit DNA binding activity of activated NF-kappa B. The inhibitory capability of OR-1246 on NF-kappa B DNA binding does not appear to be a sole mechanism, as it did not inhibit NF-kappa B activation induced by okadaic acid. Hence, catechol derivatives inhibit NF-kappa B transcription factor through multiple mechanisms, and nitecapone and OR-1246 may be useful as therapeutic agents targeting NF-kappa B.
Biochem Mol Biol Int 1994 Feb
PMID:Inhibition of NF-kappa B transcription factor by catechol derivatives. 801 35

We assessed the protection afforded against myocardial ischemia/reperfusion by nitecapone in the Langendorff heart model using male Sprague-Dawley rats. We found that when present in the perfusate 10 microM nitecapone improved the mechanical function of the heart and lowered the enzyme leakage of lactate dehydrogenase after 40 minutes of global ischemia. In nitecapone treated hearts the content of oxidized proteins and lipids (carbonyl groups and endogenous lipid fluorescent products) decreased. Nitecapone partially prevented the loss of total sulfhydryl groups and vitamin E after ischemia and reperfusion. We suggest that the mechanism of nitecapone protection most likely involves direct antioxidant action and enhancing the activity of other antioxidants.
Biochem Mol Biol Int 1993 Mar
PMID:Nitecapone protects the Langendorff perfused heart against ischemia-reperfusion injury. 848 62