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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Biosynthesis and deposition of collagen, as well as DNA and total proteins, are increased in aortae of rats after 1, 3 and 6 weeks of hypertension. 2. The maximal increase in the rate of synthesis of collagen is observed within one week of hypertension when the stress to the arterial wall is maximal. 3. Reserpine administration prevents hypertension and inhibits the increase of collagen metabolism. 4. At any time of evolution of the hypertension, a linear positive correlation is found between the collagen content in the aorta and the level of blood pressure. 5. These data suggest that synthesis of matrix components by the arterial smooth-muscle cells is controlled by variation in the blood pressure level and is not a direct consequence of circulating humoral factors liberated by the ischaemic kidney.
Clin Sci Mol Med Suppl 1978 Dec
PMID:The relationship between blood pressure and aortic collagen metabolism in renal hypertensive rats. 28 65

Reserpine administration has been shown to increase striatal tissue levels of neurotensin with a time course similar to that for striatal dopamine depletion. To determine whether reserpine treatment may increase striatal neurotensin synthesis we have examined striatal neurotensin mRNA levels using in situ hybridization histochemistry. The number of striatal cells expressing neurotensin mRNA was increased 6 h, but not 1 h, after reserpine administration. Thus, the increase in striatal tissue levels of neurotensin after reserpine may be due in part to an increase in peptide synthesis.
Brain Res Mol Brain Res 1992 Feb
PMID:Reserpine increases striatal neurotensin mRNA levels. 131 7

Expression of beta 1-adrenergic receptors and guanine nucleotide-binding proteins in rat submandibular glands was determined after reserpine administration and sympathetic denervation. Pretreatment of rats with reserpine resulted in up-regulation of the density of beta 1-adrenergic receptors and the immunoreactivity of the 64-kDa species of beta 1-adrenergic receptor in submandibular membranes, by 2.6 +/- 0.3-fold (eight experiments), within 7 days. Steady state levels of beta 1-adrenergic receptor mRNA quantified by DNA-excess solution hybridization were 0.15 +/- 0.03 amol of beta 1-adrenergic receptor mRNA/micrograms of total cellular RNA (six experiments). beta 1-Adrenergic receptor mRNA increased by 50% within 8 hr after pretreatment with reserpine. Maximal levels of 0.37 +/- 0.04 amol of beta 1-adrenergic receptor mRNA/micrograms of RNA were attained by 4 days and these levels were sustained for the next 3 days (six experiments). Northern blot hybridization also revealed a 3-fold increase in the 2.5-kilobase beta 1-adrenergic receptor mRNA transcript, which was equivalent in magnitude to that determined by solution hybridization. Reserpine pretreatment also affected steady state levels of submandibular guanine nucleotide-binding proteins. Two immunoreactive forms of the alpha subunit of Gs, migrating as 42 kDa (major) and 50 kDa (minor), were detected in salivary membranes. The immunoreactivity of the 42-kDa species of Gs alpha declined by 50% after 7 days of continuous daily injections of reserpine. In contrast, steady state levels of Gi alpha 2 (41 kDa), Go (39 kDa), and G beta 2 (35 kDa) and their mRNAs in submandibular membranes were unaffected by reserpine pretreatment. The rate of beta 1-adrenergic receptor gene transcription assessed by nuclear run-on transcription assay in nuclei of submandibular glands was not altered by reserpine pretreatment. However, reserpine had a dramatic effect on the half-life of beta 1-adrenergic receptor mRNA in submandibular glands. The half-life of beta 1-adrenergic receptor mRNA in control submandibular glands was 3.5 hr, whereas it increased to 8 hr in reserpine-pretreated glands. Reserpine-promoted stabilization of beta 1-adrenergic receptor mRNA provides a mechanism for up-regulation of postjunctional beta 1-adrenergic receptors in sympathetically innervated tissues.
Mol Pharmacol 1992 Dec
PMID:Effects of chemical and surgical sympathectomy on expression of beta-adrenergic receptors and guanine nucleotide-binding proteins in rat submandibular glands. 133 18

The levels of tyrosine hydroxylase and galanin mRNA were measured by in situ hybridization histochemistry in the rat locus coeruleus after repeated (21 days) administration of desmethylimipramine (10 mg/kg/day), of reserpine (0.25 mg/kg/day), of coadministered desmethylimipramine and reserpine, or of vehicle. Reserpine administration resulted in increased levels of both tyrosine hydroxylase and galanin mRNAs in locus coeruleus neurons as compared to vehicle-treated controls. Administration of desmethylimipramine alone failed to alter either the tyrosine hydroxylase or galanin mRNA. However, coadministration of desmethylimipramine with reserpine blocked the elevation in tyrosine hydroxylase mRNA induced by reserpine alone.
Brain Res Mol Brain Res 1991 Jul
PMID:Repeated administration of desmethylimipramine blocks the reserpine-induced increase in tyrosine hydroxylase mRNA in locus coeruleus neurons of the rat. 171 8

Transsynaptic neurogenic activity and reserpine are two signals that cause the proenkephalin (Penk) gene to alter the levels of preproenkephalin (PPenk) mRNA and enkephalin-containing (EC) peptides. In the Syrian hamster adrenal, but not in rat adrenal, both of these signals appear to be positive activators of Penk gene expression. The separate and combined effects of reserpine and denervation on EC peptides and catecholamine systems were investigated in the adrenal of the hamster, a species with relatively high medullary PPenk mRNA and EC peptide levels. Unilateral adrenal denervation resulted in a rapid decrease in PPenk mRNA levels of 54% after 2 days, and by 11 days 90% of Penk mRNA had disappeared. After 4 days both EC peptide and PPenk mRNA levels fell in parallel, whereas total RNA and soluble protein levels were unchanged. Denervation had no effect on TH mRNA levels until 8 days after surgery, and after 11 days both TH mRNA and catecholamine levels had decreased by 35-45%. Reserpine produced a dose- and time-dependent depletion of EC peptides and catecholamines. One day after 5 mg/kg reserpine (given subcutaneously on each of 2 consecutive days), EC peptides were reduced by 80%, norepinephrine by 79%, and epinphrine by greater than 95%. By 4 days after treatment, EC peptides and catecholamines slightly exceeded or had returned to control (concurrent vehicle treatment) values. PPenk mRNA levels, as measured by solution hybridization, were doubled (206 +/- 17%, mean +/- standard error) by day 4. Tyrosine hydroxylase (TH) mRNA levels were increased nearly 7-fold (686 +/- 71%) 24 hr after the first reserpine dose and declined thereafter. Northern blot analysis demonstrated that reserpine did not alter the size of either PPenk or TH mRNAs. Size exclusion chromatography showed a small (20%) reserpine-induced increase in processing of high molecular weight Penk-like peptides. The effects of reserpine, which increases PPenk mRNA, EC peptides, and TH mRNA, were completely blocked by unilateral denervation, whereas the contralateral innervated gland showed the expected responses. The co-localized EC peptide and catecholamine systems, as reflected in their mRNAs, respond differently in both time sequence and magnitude to reserpine and to denervation. Our results support a critical role, in vivo, for transsynaptic mechanisms in the maintenance of the high levels of Penk gene expression in this species and for the positive activation (mediated by reflex neurogenic stimulation) of reserpine on Penk and TH gene expression.
Mol Pharmacol 1991 Oct
PMID:Transsynaptic activity regulates proenkephalin and tyrosine hydroxylase gene expression and the response to reserpine in the hamster adrenal. 171 19

The existence of a mycoplasmal arginine deiminase which catalyzes the conversion of L-arginine to L-citrulline has been postulated. Here we show the partial amino acid sequence of arginine deiminase of Mycoplasma arginini and the complete nucleotide sequence of the arginine deiminase gene of M. arginini. The open reading frame deduced from this sequence consists of 1,230 bp encoding 410 amino acids. The mature form of this enzyme contains 409 amino acids after the deletion of the first methionine. In this open reading frame, TGA nonsense codons are used as tryptophan codons; this usage was verified by determination of the amino acid sequence. The molecular weight of the enzyme calculated from the deduced amino acid sequence is 46,372. Recently, the nucleotide sequence of the arginine deiminase gene of M. arginini was reported by Kondo et al. (K. Kondo, H. Sone, H. Yoshida, T. Toida, K. Kanatani, Y.-M. Hong N. Nishino, and J. Tanaka, Mol. Gen. Genet. 221:81-86, 1990). However, their sequence differed from ours in several places and especially at the C terminus.
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PMID:Cloning and nucleotide sequence of the gene encoding arginine deiminase of Mycoplasma arginini. 222 48

The catecholamine uptake inhibitor tetrabenazine (TBZ) binds to a high affinity site on the chromaffin granule membrane, presumably on the monoamine transporter. The hydrophobicity of the TBZ-binding site was investigated by comparing the potency of drugs to displace [3H]dihydrotetrabenazine (TBZOH), a ligand of the TBZ-binding site, with the lipophilicity of these drugs reflected by their octanol/buffer apparent partition coefficient (P o/b). Drugs tested were five substrates of the transporter, seven TBZ derivatives, and the inhibitors reserpine, haloperidol, and chlorpromazine. The validity of apparent P o/b as an index of lipophilicity was shown by measuring drug partitioning between buffer and chromaffin granule membranes. For most of the inhibitors tested, octanol/buffer and membrane/buffer apparent partition coefficients were correlated. For substrates of uptake and TBZ derivatives, the potency of a compound to displace [3H]TBZOH from its binding site was correlated to its apparent P o/b. This relationship was valid over a range of 5 orders of magnitude. These data are interpreted as indicating that the TBZ-binding site is hydrophobic and is in equilibrium with the ligand present in the membrane phase, and that substrates and TBZ derivatives are characterized by an equal intrinsic affinity for this site of about 1 microM. The 3-fold difference in affinity observed between alpha- and beta-diastereoisomers of TBZOH was accounted for by a similar difference in apparent P o/b. Reserpine, haloperidol, and chlorpromazine have much lower intrinsic affinity for the TBZ-binding site.
Mol Pharmacol 1988 Jan
PMID:Hydrophobicity of the tetrabenazine-binding site of the chromaffin granule monoamine transporter. 333 49

1. Catecholamines are transported into chromaffin granules via a carrier-mediated, active-transport process which is inhibited by micromolar concentrations of the sulfhydryl reagent, N-ethylmaleimide (NEM). Reserpine is a very potent, competitive inhibitor of the catecholamine transporter and can be used to investigate the characteristics of the catecholamine transporter. 2. The purpose of this study was to determine whether [3H]reserpine binding to the catecholamine transporter present in chromaffin granule membranes isolated from bovine adrenal glands was also inhibited by NEM and, if so, whether this was a direct or an indirect effect of NEM on the catecholamine transporter. 3. Both [3H]norepinephrine transport into and [3H]reserpine binding to the chromaffin granule ghosts isolated from bovine adrenal glands are inhibited by NEM, with IC50 values of 0.63 +/- 0.02 and 2.8 +/- 0.66 microM, respectively. 4. Mg and ATP protected both the [3H]norepinephrine transport into the ghosts and the [3H]reserpine binding to the transporter from inhibition by NEM, shifting the IC50 values to 260 +/- 43 and 120 +/- 29 microM, respectively. 5. NEM inhibition of the catecholamine transport and reserpine binding appears to be due to an action on the proton translocator associated with the Mg ATPase enzyme rather than a direct action on the catecholamine transporter since (a) the concentration of NEM required to inhibit formation of a membrane potential is similar to that required to inhibit [3H]norepinephrine transport into and [3H]reserpine binding to the ghosts and (b) Mg and ATP protected the proton translocation and [3H]norepinephrine transport into the ghosts, and [3H]reserpine binding to the ghosts, from inhibition by NEM.(ABSTRACT TRUNCATED AT 250 WORDS)
Cell Mol Neurobiol 1988 Jun
PMID:Effects of the sulfhydryl reagent N-ethylmaleimide on reserpine binding to the catecholamine transporter in chromaffin granule membranes. 340 68

The binding of receptor specific radioligands to autonomic receptors in the rat submandibular gland was characterized after chronic drug administration and surgical sympathetic denervation. Reserpine administration resulted in an up-regulation of both alpha 2-adrenergic receptors labeled by [3H]clonidine and beta 1-adrenergic receptors labeled by [3H]dihydroalprenolol. The increase in alpha 2-receptors was half-maximal 24 hr after a single injection of reserpine, and was about 10-fold greater than control after seven daily injections. By contrast, the beta-adrenergic receptor density was the same as control after 3 days of reserpine administration, but within 7 days was about 2-fold greater than control. Guanethidine or yohimbine administration also resulted in an up-regulation of alpha 2-adrenergic receptors. Reserpine administration or unilateral superior cervical ganglionectomy increased the density of alpha 1-adrenergic receptor binding sites 24-80%. Norepinephrine and methoxamine, but not clonidine, caused potassium to be released from submandibular gland slices. Prazosin, but not yohimbine, blocked this response to norepinephrine, indicating that the response was mediated by alpha 1-adrenergic receptors. Potassium release elicited by alpha 1-agonists was augmented in slices from animals that received reserpine. Neither drug treatment nor sympathetic denervation altered muscarinic cholinergic receptor binding. The densities of muscarinic and beta-adrenergic receptors were found to be 23-51% higher in glands from female rats than in glands from male rats.
Mol Pharmacol 1982 Jan
PMID:Regulation of autonomic receptors in rat submandibular gland. 612 19

[3H]Reserpine bound reversibly in vitro to chromaffin granule membranes. Binding was temperature-dependent and slow, and had biphasic kinetics. The addition of ATP accelerated the kinetics, which became monophasic and comparable to those of [3H] dihydrotetrabenazine, without affecting the binding equilibrium constants. The ATP effect was related to H+ -electrochemical gradient generation by the granule membrane H+ pump. Binding of reserpine to chromaffin granule membranes occurred on two classes of sites: R1, Bmax = 7 pmoles/mg of protein and KD = 0.7 nM, and R2, Bmax = 60 pmoles/mg of protein and KD = 25 nM. Sites R2 were considered to be equivalent to [3H] dihydrotetrabenazine binding sites, as the densities of the R2 and the [3H] dihydrotetrabenazine binding sites were similar and because tetrabenazine displaced reserpine from R2 sites. Sites R1 were tetrabenazine-resistant; they were involved in monoamine uptake, since their KD values were similar to the KI values of reserpine for noradrenaline uptake. Sites R1 were less abundant than sites R2 on chromaffin granule membranes, but they were present at the same concentration in intact chromaffin granules. We propose that the monoamine carrier exists in two forms: (a) an active form bearing both high- and low-affinity sites for reserpine and (b) an inactive form with only the low-affinity R2 sites.
Mol Pharmacol 1984 Jan
PMID:Reserpine binding to bovine chromaffin granule membranes. Characterization and comparison with dihydrotetrabenazine binding. 670 29


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