Gene/Protein Disease Symptom Drug Enzyme Compound
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Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
Methods Mol Biol 2009
PMID:Use of non-radioactive detection method for north- and southwestern blot. 1937 81

Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here we describe the use of a North-western and South-western blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
Methods Mol Biol 2015
PMID:Use of Nonradioactive Detection Method for North- and South-Western Blot. 2613 55