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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of biologically low temperature (12 degrees C) on the parameters of microbial population such as survival, catalase activity and its isoenzyme spectrum have been investigated on the models of Escherichia coli and Yersinia pseudotuberculosis. The quantity of nucleic acids, plasmid spectrum and temperature effect on the level of plasmid DNA spiralization were studied under these conditions. The process of molecular genetic adaptation of bacterial populations having the broad temperature limits of growth and demonstrating the increased genetical expression when affected by the biologically low temperature has been found to be regulated on the transcriptional level. The inducible catalase isoenzymes participate in adaptation. The effect of biologically low temperature on the level of the plasmid DNA superhelicity and DNA quantity during the short period of poststress was not demonstrated.
Mol Gen Mikrobiol Virusol 1989 Sep
PMID:[Various approaches to studying molecular-genetic mechanisms of low-temperature adaptation of microbial populations]. 269 57

Using the technique of the genes probes the naturally occuring strains Y. pseudotuberculosis and Y. enterocolitica of epidemiological importance were shown to contain pCad plasmid integrated with the chromosome. The strains having integrated plasmid express all pathogenicity determinants necessary for realization of infectious process.
Mol Gen Mikrobiol Virusol 1989 May
PMID:[Integration with the chromosome--an alternative state of calcium-dependency plasmids in Yersinia]. 274 4

The Yop proteins of Yersinia are important virulence determinants. The Yop1 protein sequences of Yersinia pestis, Yersinia pseudotuberculosis, and two Yersinia enterocolitica serotypes, 0:3 and 0:8, deduced from the nucleotide sequences of the corresponding yopA genes, were compared. Most differences were found in the hydrophilic domains of the proteins, whereas the hydrophobic domains were conserved. The amino acid sequences revealed a signal sequence 25 amino acids long. No cysteine residues were present, even though Yop1 forms a polymeric structure. The transcription startpoint of yopA was determined by primer extension. The coding region and transcription startpoint were separated by a leader sequence 270 nucleotides long. The yopA promoter sequence of Y.pestis is identical to the corresponding sequence of Y. pseudotuberculosis and transcription studies revealed that this promoter is active in Y.pestis. Thus, the inability of Y.pestis to express the Yop1 protein is due to a single base pair deletion in the coding region of the yopA gene of Y.pestis.
Mol Microbiol 1989 Apr
PMID:Analysis of the yopA gene encoding the Yop1 virulence determinants of Yersinia spp. 276 89

The DNA sequence of the structural gene (yopE) of one of the Yersinia pseudotuberculosis virulence plasmid-plB1-encoded proteins, Yop5, is presented. The deduced protein showed a molecular weight of 22,971 Daltons. A specific mutant, having a kanamycin-resistance fragment inserted within the yopE gene was no longer virulent for mice. The expression of the Yop5 protein is regulated at the level of transcription by temperature as well as by the Ca2+-concentration of the medium. A significant increase in the level of transcription was not detected until 45 min after a temperature shift from 26 degrees C to 37 degrees C in the absence of calcium; addition of Ca2+ inhibited the expression. The yopE promoter is under positive, as well as negative, plB1-encoded control. The positive function is solely regulated by temperature, while the regulation of the negative function involves at least five different plasmid-encoded gene loci; one of these genes encodes the V-antigen.
Mol Microbiol 1988 Jan
PMID:The virulence protein Yop5 of Yersinia pseudotuberculosis is regulated at transcriptional level by plasmid-plB1-encoded trans-acting elements controlled by temperature and calcium. 283 86

The basic Yop2b protein, encoded by the virulence plasmid pIBI of Yersinia pseudotuberculosis, is produced under Ca2+-deficient conditions. A mutant deleted for the entire yopH gene, which encodes the Yop2b protein, was found to be avirulent. Virulence could be restored by trans-complementation. The DNA-sequence of yopH predicted a 50 737 D polypeptide lacking a typical signal peptide. Transcription of yopH is regulated by both temperature and Ca2+-concentration. Mutations within the region of the virulence plasmid known to be involved in regulating gene expression in response to Ca2+ abolished transcription of yopH. Other temperature-sensitive mutations in the Ca2+-regulatory locus showed a high level of transcription regardless of Ca2+-concentration. These responses were similar to those of the yopE gene. The promoter region of the yopE and yopH genes were compared and four conserved motifs identified.
Mol Microbiol 1988 Mar
PMID:The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription. 283 14

The plasmids pCG86 and RP4elt coding for thermolabile enterotoxins of Escherichia coli (LT) were transferred in conjugation to Yersinia enterocolitica and Yersinia pseudotuberculosis cells. Both plasmids were stably inherited by the recipient cells. The elt genes of the toxins were expressed in Yersinia cells at the level comparable to the one registered in Escherichia coli cells. In the broth cultures of transconjugant cells the major part of LT toxin is bound with cells (74-97%). The obtained data may serve an experimental basis in favour of possibility of Ent+ strains of Yersinia enterocolitica and Yersinia pseudotuberculosis formation in nature and expediency of search and diagnosing of such strains.
Mol Gen Mikrobiol Virusol 1988 Aug
PMID:[Production of thermolabile enterotoxins of Escherichia coli by Yersinia enterocolitica and Yersinia pseudotuberculosis]. 305 60

The clones of two types (T+ and T-) have been identified among the strains of Y. pseudotuberculosis. The difference in the morphology of the clones is based on the ability of T+ clones to agglutinate in the process of growth. The identified clones are different in calcium dependence, in the ability to agglutinate and in their virulence for the laboratory animals. The differences have been proved to be connected with the presence of a 45 Md plasmid in T+ cells. Replication of this plasmid is suppressed by the acridine orange dye in concentrations of 12.5 or 25.0 mkg X ml-1. The plasmid is spontaneously lost from the strains during their continuous storage. The microscopy of colonies permits the selection of clones with the parental phenotype from the populations having lost the 45 Md plasmid.
Mol Gen Mikrobiol Virusol 1986 Oct
PMID:[Various characteristics of Yersinia pseudotuberculosis determined by plasmid 45 Md associated with calcium dependence]. 379 52

Yersinia pestis plasmids having inserted Tn-movable elements and determining pesticinogenicity, Ca2+-dependence, production of mice toxin and fraction I have been mobilized for conjugal transfer into Y. pestis, Y. pseudotuberculosis or E. coli cells by plasmid pKA7. Plasmid determinants have been transferred with the frequencies 10-4-10-7 per donor cell. The plasmid DNA of high molecular mass has been identified in transconjugant cells. Plasmid mobilization in Yersinia pestis is supposed to be due to cointegrate formation of yersinia plasmids with R-plasmids.
Mol Gen Mikrobiol Virusol 1986 May
PMID:[Mobilization of plasmid determinants of Yersinia pestis using the plasmid pKA7]. 379 53

The virulence plasmid-encoded YopE cytotoxin of Yersinia pseudotuberculosis is secreted across the bacterial membranes and subsequently translocated into the eukaryotic cell. Translocation of YopE into target cells was recently shown to be polarized and only occurred at the zone of contact between the pathogen and the eukaryotic cell. Immunogold electron microscopy on cryosectioned Y. pseudotuberculosis revealed that YopE is secreted and deposited on the bacterial cell surface when the bacteria are grown in Ca(2+)-depleted media at 37 degrees C. No YopE was detected in the cytoplasm or in the membranes. In yerA mutants which are downregulated for YopE at a post-transcriptional level, the cytotoxin could only be detected in the cytoplasm. The overall recovery of YopE from the yerA mutant strain was, however, considerably lower than from the wild-type strain. yerA had no major effect on the translation of YopE, but was found to stabilize YopE in the cytoplasm. YerA was shown to specifically interact with YopE in the cytoplasm in vivo and this binding also correlated with YopE secretion. Targeting of YopE to the secretion loci as well as translocation of YopE into HeLa cells occurred also in the absence of YerA. Based on our findings, we suggest that YerA by binding to YopE stabilizes and maintains the cytotoxin in a secretion-competent conformation.
Mol Microbiol 1995 May
PMID:The chaperone-like protein YerA of Yersinia pseudotuberculosis stabilizes YopE in the cytoplasm but is dispensible for targeting to the secretion loci. 747 59

The genomes of three main biovars of Yersinia pestis were subjected to restriction fragment length polymorphism analysis using I-CeuI endonuclease. I-CeuI which is encoded by a mobile intron in Chlamydomonas engamenans recognizes a 25-bp site in the ribosomal RNA rrl gene and cuts DNA of most representatives of Enterobacteriaceae into seven fragments corresponding to the presence of seven rrn-operons. Glycerol-positive Y. pestis strains (biovars antiqua and mediaevalis) contain seven ribosomal operons which can be recognized by I-CeuI endonuclease. However, glycerol-negative strains of Y. pestis biovar orientalis expose only six restriction sites for I-CeuI. The restriction fragment length polymorphism patterns obtained with I-CeuI make it possible to distinguish between three biovars of Y. pestis. Use of another rare cutting restriction enzyme, Bln/I, permits differentiation between pigment-adsorbing and avirulent non-pigment-adsorbing Y. pestis. Still, due to homologous recombination between the two copies of IS 100 insertion sequence bracketing the pgm-locus, the mechanism of deletions in the pgm-locus seems to be confined only to strains of biovars antiqua and mediaevalis, and can be different in Y. pestis strains of biovar orientalis. The I-CeuI restriction patterns of two Yersinia strains isolated within a ten-year period in the port of St. Petersburg and originally identified as Y. pseudotuberculosis 01 turned out to be related to typical representatives of Y. pestis biovar antiqua. These strains could be exported from the same source or circulate among Rattus norvegicus population of the port as non-pigment-adsorbing avirulent immunogenic clone.
Mol Gen Mikrobiol Virusol
PMID:The established Yersinia pestis biovars are characterized by typical patterns of I-CeuI restriction fragment length polymorphism. 747 35


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