Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of biologically low temperature (12 degrees C) on the parameters of microbial population such as survival, catalase activity and its isoenzyme spectrum have been investigated on the models of Escherichia coli and Yersinia pseudotuberculosis. The quantity of nucleic acids, plasmid spectrum and temperature effect on the level of plasmid DNA spiralization were studied under these conditions. The process of molecular genetic adaptation of bacterial populations having the broad temperature limits of growth and demonstrating the increased genetical expression when affected by the biologically low temperature has been found to be regulated on the transcriptional level. The inducible catalase isoenzymes participate in adaptation. The effect of biologically low temperature on the level of the plasmid DNA superhelicity and DNA quantity during the short period of poststress was not demonstrated.
Mol Gen Mikrobiol Virusol 1989 Sep
PMID:[Various approaches to studying molecular-genetic mechanisms of low-temperature adaptation of microbial populations]. 269 57

The results of the plasmid screening of Yersinia pestis strains isolated from four autonomous focuses on the northern border of the Central Asian zone of plague natural focality are presented. The plasmid profile of Yersinia pestis strains from the focuses is characterized as stable and independent of the source and time of strain isolation. The peculiar characteristic of the strains isolated in Tuva is the presence of an "additional" 15-16 Md plasmid in those strains.
Mol Gen Mikrobiol Virusol 1989 Apr
PMID:[Results of screening of plasmids of Yersinia pestis strains from various regions of endemic central-asian plague focus]. 274 98

The Yop proteins of Yersinia are important virulence determinants. The Yop1 protein sequences of Yersinia pestis, Yersinia pseudotuberculosis, and two Yersinia enterocolitica serotypes, 0:3 and 0:8, deduced from the nucleotide sequences of the corresponding yopA genes, were compared. Most differences were found in the hydrophilic domains of the proteins, whereas the hydrophobic domains were conserved. The amino acid sequences revealed a signal sequence 25 amino acids long. No cysteine residues were present, even though Yop1 forms a polymeric structure. The transcription startpoint of yopA was determined by primer extension. The coding region and transcription startpoint were separated by a leader sequence 270 nucleotides long. The yopA promoter sequence of Y.pestis is identical to the corresponding sequence of Y. pseudotuberculosis and transcription studies revealed that this promoter is active in Y.pestis. Thus, the inability of Y.pestis to express the Yop1 protein is due to a single base pair deletion in the coding region of the yopA gene of Y.pestis.
Mol Microbiol 1989 Apr
PMID:Analysis of the yopA gene encoding the Yop1 virulence determinants of Yersinia spp. 276 89

Hemin and hemoglobin are found to be adequate sources of iron for growth of Yersinia pestis. The mechanism of their assimilation is similar and consists of absorbtion of the free intact hem molecule.
Mol Gen Mikrobiol Virusol 1989 Jun
PMID:[Utilization of hemoglobin by the plague microbe]. 281 99

The relation of Yersinia pestis calcium dependence plasmid (pCad) to known Inc FI (F'lac, R386, pOX38) and IncFV (F0lac) plasmids has been studied. Evidence that plasmid pCad of Yersinia pestis belongs to FI incompatibility group is presented.
Mol Gen Mikrobiol Virusol 1987 Oct
PMID:[Incompatibility of the Cad plasmid from Yersinia pestis with plasmids of the IncFI group]. 282 35

The DNA sequence of the structural gene (yopE) of one of the Yersinia pseudotuberculosis virulence plasmid-plB1-encoded proteins, Yop5, is presented. The deduced protein showed a molecular weight of 22,971 Daltons. A specific mutant, having a kanamycin-resistance fragment inserted within the yopE gene was no longer virulent for mice. The expression of the Yop5 protein is regulated at the level of transcription by temperature as well as by the Ca2+-concentration of the medium. A significant increase in the level of transcription was not detected until 45 min after a temperature shift from 26 degrees C to 37 degrees C in the absence of calcium; addition of Ca2+ inhibited the expression. The yopE promoter is under positive, as well as negative, plB1-encoded control. The positive function is solely regulated by temperature, while the regulation of the negative function involves at least five different plasmid-encoded gene loci; one of these genes encodes the V-antigen.
Mol Microbiol 1988 Jan
PMID:The virulence protein Yop5 of Yersinia pseudotuberculosis is regulated at transcriptional level by plasmid-plB1-encoded trans-acting elements controlled by temperature and calcium. 283 86

The basic Yop2b protein, encoded by the virulence plasmid pIBI of Yersinia pseudotuberculosis, is produced under Ca2+-deficient conditions. A mutant deleted for the entire yopH gene, which encodes the Yop2b protein, was found to be avirulent. Virulence could be restored by trans-complementation. The DNA-sequence of yopH predicted a 50 737 D polypeptide lacking a typical signal peptide. Transcription of yopH is regulated by both temperature and Ca2+-concentration. Mutations within the region of the virulence plasmid known to be involved in regulating gene expression in response to Ca2+ abolished transcription of yopH. Other temperature-sensitive mutations in the Ca2+-regulatory locus showed a high level of transcription regardless of Ca2+-concentration. These responses were similar to those of the yopE gene. The promoter region of the yopE and yopH genes were compared and four conserved motifs identified.
Mol Microbiol 1988 Mar
PMID:The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription. 283 14

Yersinia pestis cells are shown to be sensitive to bacteriophage Mu cts62 infection. Lysis of bacteria has been shown to be more efficient on solid nutrient medium than in LB broth. 10(-5) pfu per ml is the maximal concentration of bacteriophage particles yielded from the broth cultures of bacteria. Moi 0.1 has been used to obtain such yields of bacteriophage. Lysogenization of Yersinia pestis cells has not been achieved when the standard methods of bacteriophage infection have been used. It was accomplished by the conjugal transfer of plasmid RP4::MU cts62 to Yersinia pestis from Escherichia coli. The deficiency of Yersinia pestis in producing bacteriophage Mu cts62 mature particles during the lytic cycle of bacteriophage is discussed.
Mol Gen Mikrobiol Virusol 1985 Sep
PMID:[Interaction of Yersinia pestis bacteria with bacteriophage mu]. 294 25

Transposons Tn1, Tn7, Tn9, Tn10 have been inserted into each of three known plasmids in Yersinia pestis and have been shown to mutagenize the different plasmid genes. The marked plasmids are shown to be transduced by bacteriophage P1 cml clr 100 ts in intrageneric crosses. The genes of 61-65 Md plasmid were found to be impaired with high frequencies by Tn9 and Tn10 insertions blocking the synthesis of fraction I antigen. The genes are also impaired in course of transduction of transposon marked plasmids.
Mol Gen Mikrobiol Virusol 1985 Oct
PMID:[Isolation of Yersinia pestis plasmids with transposon markers]. 302 77

Genes of resistance to some aminoglycoside antibiotics from plasmid R323 were transduced by bacteriophage P1 cml clr100 ts in Yersinia pestis. The resistance markers were capable of insertion into the chromosome or plasmid in the recipient cells causing the mutagenic effect. The results obtained suggest the transposon nature of plasmid fragment coding for gentamicin-kanamycin resistance.
Mol Gen Mikrobiol Virusol 1985 Mar
PMID:[Mutagenic effect during transduction of (Gm-Km)R markers of the R323 plasmid in Yersinia pestis]. 302 95


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