Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

After immunization of rabbits the antiserum was prepared against purified reverse transcriptase (revertase) from avian myeloblastosis virus (AMV). The antiserum demonstrated enzymeneutralizing antibody activity that was associated with ummunoglobulin G fraction but not with IgM. The high antigenicity of AMV revertase for rabbits was shown. The active antiserum was obtained after 4 immunizations of rabbit with approximately 20 microgram of the enzyme. Non-specific revertase inhibitors were found in normal rabbit serum, which were absent in IgG fraction from this serum. The revertase activity of Rauscher leukemia virus (RLV) and Visna virus was not neutralized by antisera against AMV polymerase. This work was supported by the project "Revertase".
Mol Biol (Mosk)
PMID:[Immunologic aspects of preparation of antiserum to the reverse transcriptase from avian myeloblastosis virus]. 8 6

Low molecular weight chromatin peptides exert a dose-dependent inhibition of Dimethylsulfoxide (DMSO)-induced erythroid differentiation of murine Friend Leukemia Cells (FLC). This effect correlates with the degree of purification of the peptide fractions. Crot analysis of globin mRNA amounts in DMSO-treated FLC given the peptides showed a 4-5-fold decrease of messenger RNA in the cytoplasma with no nuclear storage of globin transcripts. Spectrin accumulation in "induced" FLC is inhibited as well. The effects of the peptides on erythroid markers are reversible upon removal of the compounds. They also appear to be specific for induced gene expression as (1) no effects are observed on cell growth and RNA synthesis in normal non-differentiating cell lines; and (2) no changes have been detected with regard to the expression of integrated viral genes coding for continuous shedding of viral particles.
Mol Biol Rep 1979 Dec 31
PMID:Effects of a chromatin low molecular weight peptidic fraction on differentiation markers and virus production in Friend leukemia cells. 9 93

Experimental evidence for the presence and biosynthesis of subviral, leukemogenic particles in the isolated mitochondria of spleen cells of mice infected with Rauscher murine leukemia (RML) virus is presented. These subviral particles sediment at a density of 1.27-1.29 g/ml and induce splenomegaly and RML three weeks after i.v. or i.p. administration to white mice. Virosomes have been labelled with [32P]phosphate in the isolated mitochondria from RML spleen cells and high molecular weight (70S) [32P]RNA has been isolated from these subviral, leukemogenic particles. Rauscher virus group specific antigens were detected by immunodiffusion in the inner membrane and matrix fraction of the mitochondria of RML spleen cells. These results together with our earlier findings strongly suggest that mitochondria of the transformed cells participate in the biosynthesis of RNA tumor viruses. Possible mechanism of the penetration of viral genetic information of RNA tumor viruses into mitochondria of tumor cells in vivo is discussed.
Mol Cell Biochem 1977 Feb 04
PMID:Biosynthesis of subviral oncogenic particles (virosomes) in mitochondria of Rous sarcoma and Rauscher murine leukemia cells. 19 96

1. A granulocyte-rich fraction was isolated from blood of a patient with chronic granulocytic leukaemia and from blood of a normal subject and the cells were disrupted in isotonic sucrose. The nuclei were removed by low-speed centrifugation and the post-nuclear supernatant was fractionated by centrifugation on sucrose density gradients. 2. The subcellular organelles in the gradients were detected with marker enzymes by the use of highly sensitive assay techniques. Similar results were obtained with granulocytes from both subjects. 3. Unsaturated vitamin B12-binding proteins were almost exclusively localized to the specific granules. Chromatographic analysis of these proteins showed them to have approximately equal proportions of transcobalamins I and III. Vitamin B12 assayed microbiologically could not be detected in the specific granule fractions.
Clin Sci Mol Med 1975 Aug
PMID:Analytical subcellular fractionation of human granulocytes with reference to the localization of vitamin B12-binding proteins. 23 84

Wild mice (Sk, Hz-Vl, Hz-IV Om, Mol.A, Fu, Te, and Sn) trapped in various areas of Japan were crossed with mice of inbred strains (C57BL/6, C57L, BALB/c, and C57BL/10), and their progeny were infected with NB-tropic Friend nurine leukemia virus. Ten days after infection, the spleens were weighed, examined for macroscopic focal lesions, and assayed for infectious virus by the XC test. Genetic analysis indicated that 4 of 8 mice tested had a dominant gene that suppresses the virus replication; the gene resembles the Fv-4' allele. No mice with the Fv-2' allele were found.
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PMID:Genetic resistance in Japanese wild mice (Mus musculus molossinus) to an NB-tropic Friend murine leukemia virus. 28 Jul 14

Activated lymphocytes release numerous products which are either synthesized de novo or in increased amounts; some of these products play a role in the regulation of the immune response and are designated as mediators of cellular immune reactions or lymphokines. The first lymphokine described was the macrophage migration inhibitory factor (MIF) which has been studied most extensively with regard to its chemical and biological properties. Using sensitive radiolabelling techniques and an antiserum against highly purified fractions of MIF we were able to identify several products of activated guinea pig lymphocytes with different molecular weights of 15.000, 30.000, 45.000, 60.000 which all had an isoelectric point of 5.2 and were all inhibitory to macrophage migration. It is suggested, that these molecules are oligomers of a common subunit of molecular weight 15.000. It was further shown, that molecules of the same physical-chemical and serological characteristics are produced by activated B-cells, L2C leukemia cells and growing fibroblasts, thus further substantiating earlier reports on the production of MIF by lymphoid and non-lymphoid cells. The described molecules were also shown not to contain determinants of the major histocompatibility complex and to be distinct from lymphotoxin, another lymphocyte activation product. It is concluded, that MIF is not a single molecule but rather a system of structurally related molecules. Their interaction with macrophages and possible relationships to macrophage activating factor is discussed.
Mol Cell Biochem 1979 Dec 14
PMID:The biochemistry and in vitro activity of soluble factors of activated lymphocytes. 39 92

Murine L1210 leukemia cells were cultured in the presence of various steroid hormones to determine their growth response. Cells maintained in medium containing glucocorticoids such as dexamethasone exhibit a 10--20% inhibition of growth, whereas progesterone of 17 beta estradiol produce a 40--50% inhibition of growth. The response is due to growth inhibition and not to any cytolytic effect of the steroids. L1210 cytoplasmic and nuclear extracts contain high affinity binding sites for [3H]dexamethasone, [3H]progesterone and [3H]17 beta-estradiol. Competition with various steroids indicate that estrogens bind to one class of cytoplasmic binding sites, while glucocorticoids and progestins bind to a second class of receptor sites. These receptors appear not be be completely responsible for growth inhibition since there is no correlation between the magnitude of growth inhibition and the intracellular concentration of receptor sites, and since the concentration of steroid required to cause significant growth inhibition exceeds those concentrations required to saturate the cytoplasmic receptors.
Mol Cell Endocrinol 1979 Jan
PMID:Steroid receptors and steroid response in cultured L1210 murine leukemia cells. 44 77

We have isolated the gp70 of a helper-independent strain of a Friend mink cell focus-inducing (MCF) virus, Fr-MCF-1. This recombinant virus, like the previously described AKR-MCF viruses, has been shown by both biological and biochemical means to be an envelope gene recombinant between Friend murine leukemia virus (F-MuLV) and a mouse xenotropic virus. Utilizing (125)I- labeled Fr-MCF-1 gp70 and antiserum prepared against an MCF strain of Moloney type-C virus (Mol-MCF(83)), we have developed a radioimmunoassay which detects immunological determinant (s)contained in the gp70s of MCF viruses derived from F-MuLV, Mol-MuLV, and AKR-MuLV. This MCF determinant(s) is not detected in the ecotropic parents of each of these MCF viruses, nor in helper-independent murine xenotropic viruses derived from Swiss or BALB/c mice. A protein partially cross-reactive with the MCF gp70 determinant(s) is detected in a replicating xenotropic virus derived from NZB mice. Utilizing this MCF gp70 specific immunoassay, we can detect a cross-reacting gene product coded for by the Friend strain of the spleen focus-forming virus (SFFV) in rat fibroblasts nonproductively infected with SFFV. The results support earlier molecular hybridization studies which indicated that the genome of SFFV contains genetic information derived from both F-MuLV and xenotropic virus, and that the xenotropic-related sequences in SFFV are highly related to those found in MCF murine type-C viruses.
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PMID:Type-specific radioimmunoassays for the gp70s of mink cell focus-inducing murine leukemia viruses: expression of a cross-reacting antigen in cells infected with the Friend strain of the spleen focus-forming virus. 70 51

1. The properties of ferritin in serum have been compared with those of ferritin from a number of tissues including blood cells. On anion-exchange chromatography with DEAE-Sephadex, the behaviour of human heart ferritin is different from that of liver, kidney or spleen ferritin. Reticulocyte ferritin appears to have similar characteristics to heart ferritin. 2. Serum ferritin from normal subjects and patients with various degrees of iron load, leukaemia or liver disease all have a much lower affinity for the anion-exchange column that any tissue ferritin, suggesting a difference in isoelectric point. The elution point of serum ferritin from patients with acute myeloblastic leukaemia is significantly different from normal. 3. Density gradient centrifugation in sucrose showed that ferritin in leucocyte extracts and partially purified ferritin from the serum of two patients with iron overload behaved as apoferritin rather than the iron-rich protein. 4. The results suggest that ferritin is modified during its entry into the plasma and that even in cases of iron overload the iron content of serum ferritin may be low. The findings are of importance in considering the origin of plasma ferritin, the clearance of ferritin from plasma and its role in iron metabolism.
Clin Sci Mol Med 1975 May
PMID:The characteristics of ferritin from human tissues, serum and blood cells. 116 59

The ability of Moloney murine leukemia virus to accelerate lymphomagenesis in E mu-myc transgenic mice is frequently associated with proviral integration within a locus denoted bmi-1. This locus contains not only the bmi-1 gene implicated as a collaborator with myc in lymphomagenesis but also just upstream an unknown gene denoted bup. The nucleotide sequence reported here for bup cDNA and flanking genomic sequences reveals that this widely expressed gene comprises at least 7 exons and potentially encodes a polypeptide of 195 amino acid residues. Computer searches with this polypeptide sequence revealed no close homolog in the databases, nor any conserved motifs, and it is unrelated to the product of the mel-13 gene, which lies just upstream from the bmi-1 homolog mel-18.
Mol Biol Rep 1992 Nov
PMID:Nucleotide sequence of bup, an upstream gene in the bmi-1 proviral insertion locus. 128 75


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