Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Synthesis of virus-specific RNA in cells infected with influenza B virus was studied by means of PAGE analysis of RNA hybrid duplexes or nucleocapsid-associated RNA. A switch from an "early" to "late" pattern was registered in the relative rates of synthesis of the corresponding mRNA as well as in the synthesis of vRNA segments 7 and 8. Contrary to the pattern described earlier for influenza A virus, the relative rate of synthesis of mRNA 5 was increased at the late stage of the replication cycle; besides, the efficiency of transcription of the polymerase genes was higher. Partial suppression of protein synthesis with moderate concentration of cycloheximide at a late stage of infection resulted in a differential inhibition of vRNA segments synthesis and stimulation of mRNA synthesis, leading to restoration of an "early" pattern in both cases. The results confirm the general outline of regulation as presented for influenza A virus in an earlier publication and reveal several peculiarities in regulation of influenza B viral RNA synthesis.
Mol Gen Mikrobiol Virusol 1989 Oct
PMID:[Synthesis of virus-specific RNA in cells infected with influenza B virus]. 261 71

The full-length copy of the hemagglutinin gene of influenza virus was inserted into M13 phage DNA. The DNA sequence coding for the hydrophobic prepeptide was removed from the gene by oligonucleotide-directed mutagenesis. The possibilities of expression of the full-length and mutant genes in E. coli were investigated. The beta-galactosidase-hemagglutinin fusion proteins were isolated. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess of viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.
Mol Biol (Mosk)
PMID:[Cloning and expression of the hemagglutinin gene of influenza virus subtype H1 in Escherichia coli]. 267 77

The influenza virus RNA replication and transcription have been extensively studied both in infected cell and in vitro systems. The review presents the analysis of experimental data on the molecular mechanisms for influenza virus RNA replication and transcription as well as on the regulation of the synthesis of three kinds of virus-specific RNAs (vRNA, cRNA and mRNA) and their interrelationships. A hypothetical pattern of regulation is presented, providing an explanation for change in the rate of synthesis of RNA segments and their transcripts in the course of the replication cycle.
Mol Gen Mikrobiol Virusol 1989 Aug
PMID:[The mechanism of RNA synthesis in the influenza virus]. 268 24

The complete primary structure of cDNA for hemagglutinin gene of influenza virus A/FPV/weybridge/27 subtype H7 has been determined. Its comparison with the structures of analogous genes from other strains of the same subtype has shown 75% of base changes resulting in silent mutations. This suggests the weak immunological pressing in course of evolution of this subtype strains. The reason for apathogenicity of this avian strain is supposed to be elimination of a glycosylation site present in the strain A/FPV/Rostock/34. The possibility of using the obtained data for construction of the new generation of vaccines is discussed.
Mol Gen Mikrobiol Virusol 1989 May
PMID:[Primary structure of the avian influenza virus A/FVP/Weybridge hemagglutinin gene]. 274 2

The data are presented on the possibility of using the dot immunosorption technique for identification of influenza viral antigens in the tissues of infected animal in course of infection. The possibility of quantitative evaluation of the viral antigen contents in the tissues of infected animal permits to use the technique for evaluation of dynamics and rate of viral antigens excretion in course of infection. The information might be useful for studying the pathogenicity of influenza as well as for evaluation of efficiency of immunocorrecting and antiviral preparations. Auxiliary analysis of antigen-containing material by immunoblotting technique has confirmed the viral nature of identified polypeptides. In the future the proposed method may be used to study the viral antigenic structure in infected tissues dependent of the location and time of viral antigens isolation.
Mol Gen Mikrobiol Virusol 1989 May
PMID:[Detection of viral antigens in the lungs and spleen of mice infected with influenza virus]. 274 3

The two variants of influenza A/Victoria/35/72 (H3N2) virus resistant simultaneously to remantadine, deitiforin, adapromine and amantadine were obtained while passaging the virus in presence of remantadine or deitiforin. Both variants differed from the parental strain in optimal pH for hemolysis, transcriptase activity and in amino acid sequence of M2 protein. Maximal hemolytic activity of the parental strain is registered at pH 5.2, for the variants cultured in the presence of remantadine or deitiforin at pH 5.5 and 5.8, respectively. In contrast to NH4OH, remantadine and deitiforin do not exert inhibition of virus-induced hemolysis. Transcriptase activity of resistant variants is about 50% higher as compared with parental strain (enzyme source--whole virus particles or RNP). The M2 protein of the remantadine variant has 2 amino acid substitutions: 31 (Ser----Asn) and 59 (Met----Leu); the deitiforin variant has 3 substitutions: 14 (Met----Leu), 30 (Ala----Val) and 59 (Met----Leu). The phenotypic resistance of the virus seems to be determined by the mutations in the hydrophobic protein region (30,31); the other substitutions (14,59) may modify conformational structure and functional activity of the viral proteins.
Mol Gen Mikrobiol Virusol 1989 Jun
PMID:[The change in functional activity and primary structure of the M2 protein in variants of the influenza virus resistant to remantadine and deitiforin: common and individual differences from the original strain]. 281

In the presence of [3H]-nucleosides the high-labeled viral [3H]-RNAs are shown to be sorted out and not included into maturing viral particles in the cells infected by influenza virus. Finding of the "sorting out" mechanism came to be possible due to storing cells in the cold for a long time and thus permitting to accumulate [3H]-decays under the conditions of temporary interruption of infectious process. Most probably, the high-labeled [3H]-RNAs are not included into the maturing viral particles due to their radiolytic damages. The "sorting out" of high-labeled viral RNAs is evidently compensated by comparatively low-labeled RNAs from the redundant intracellular pool of v-RNAs.
Mol Gen Mikrobiol Virusol 1989 Aug
PMID:[Intracellular "sorting" of highly marked (3H)-v-RNA during maturation of influenza virus]. 281 10

3'-Azido-2',3'-dideoxythymidine (az-T) inhibited effectively the reproduction of some retroviruses; among these viruses were the four serological subgroups of sarcoma Raus virus in chicken embryo, avian myeloblastosis virus and erythroblastosis virus in chicken. This inhibition was specific towards retroviruses and practically was not observed in the case of infections DNA- and RNA-genome model viruses of vaccinia and influenza, at whose reproduction reverse transcriptase is not involved. Three other 3'-modified nucleosides did not block the above-listed retroviruses. For chickens, az-T showed low toxicity. The molecular mechanisms of the action of az-T are discussed.
Mol Biol (Mosk)
PMID:[The effect of 3'-azido-2',3'-dideoxythymidine on experimental viral infections]. 282 79

N-vinylpyrrolidone-N(2-hydroxy) acrylamide copolymer-modified porous glass was studied as a support for gel permeation chromatography. Influenza, Sendai, fowl plague viruses and rota-viruses were purified by chromatography on the support. As compared with Sepharose 4B the support shows better hydrodynamic properties and yields higher amounts of purified viruses. The support can be also applied to the t-RNA and ribosomes separation.
Mol Gen Mikrobiol Virusol 1987 Nov
PMID:[Polymer-modified silica sorbents for molecular sieve chromatography of biopolymers]. 283 49

To determine whether the tripartite leader is required for efficient translation in adenovirus-infected cells at late times of infection, we constructed recombinant adenoviruses containing the influenza virus nucleocapsid protein (NP) gene expressed under the control of the adenovirus major late promoter (MLP). We chose the NP gene because previous results showed that the influenza virus NP mRNA was an extremely effective initiator of translation in cells which were superinfected with influenza virus at late times of adenovirus infection (M. G. Katze, B. M. Detjen, B. Safer, and R. M. Krug, Mol. Cell. Biol. 6:1741-1750, 1986). The NP gene in the adenovirus recombinants was inserted downstream of an MLP that replaced part of the early (E1A) region. The resulting NP mRNAs either lacked any tripartite leader sequences or contained at their 5' ends various portions of the tripartite leader: 33, 172, or all 200 nucleotides of the leader. The relative amounts of the NP protein synthesized by the recombinants were directly proportional to the amounts of the NP mRNA made, indicating that the presence of 5' tripartite leader sequences did not enhance the translation of NP mRNA. In addition, the sizes of the polysomes containing NP mRNA were not increased by the presence of tripartite leader sequences, indicating that the initiation of translation was not enhanced by these sequences. On the other hand, the presence of tripartite leader sequences immediately downstream of the MLP did enhance the transcription of the inserted NP gene, as shown by Northern (RNA) analysis of in vivo NP mRNA levels and by in vitro runoff assays with isolated nuclei. Our results indicate that more than 33 nucleotides of the first leader segment of the tripartite leader are required for optimal transcription from the MLP.
 ...
PMID:Efficient transcription, not translation, is dependent on adenovirus tripartite leader sequences at late times of infection. 283 10


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>