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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The origin of DNA in transducing particles of bacteriophage Mu was investigated by density labelling techniques. Unlabelled plaque-forming and leu+-transducing particles were of about the same density. Preinfection labelling of DNA with 5-bromodeoxyuridine increased the density of the transducing particles, but not that of the infective ones. Postinfection labelling increased the density of the infective particles twice as much as that of the transducing particles. We conclude that half of the transducing DNA is synthesized before infection and half is synthesized after infection, similar to the results obtained with P1kc transducing phages (Ikeda and Tomizawa, 1965).
Mol Gen Genet 1979 Oct 03
PMID:The origin of the DNA in transducing particles of bacteriophage Mu. Density gradient analyses of intact phages. 16 Sep 75

In this paper, we report the construction in vitro of chimerae between lambdoid replacement vectors (Murray et al., 1977) and the miniF Apr plasmid: pSC138 (Timmis et al., 1975). lambdaF recombinants were shown to be chimerae between the lambda and the F replicons. By genetical tests, we have demonstrated that both lambda and F replication mechanisms are functional: the lambdaF recombinant behaves as a non defective plaque forming phage on lambda sensitive bacteria and establishes itself as a stable plasmid on recA F- homoimmune bacteria. In the extra-chromosomal state, the lambdaF recombinant apparently retains the controlled autonomous replication and the FI incompatibility characteristics of the F plasmid. The potential experimental uses of these phages are discussed.
Mol Gen Genet 1979 Jan 16
PMID:Construction in vitro of "phage-plasmid" chimerae: a new tool to analyse the mechanism of plasmid maintenance. 28 46

Lambda mutants capable of N-independent red-gam gene expression were isolated by selecting Fec+ plaque-forming derivatives of lambda N+ nutL- (Fec-) strains. In addition to true nutL+ reversions, three classes of second-site mutations were identified: (1) ninL deletions that remove a region containing either tL1 or both tL1 and tL2 termination signals, or only a small region (defining the rut site) just upstream from tL1, (2) new constitutive promoters that map just upstream from the tL2 termination site and which are created either by point mutations (hip) or by short insertion sequences (isp), (3) small internal deletions in gene cro. The positions and individual effects of these mutations, some of which only partially abolish termination function, provide evidence for a complex multipartite structure of the termination signals.
Mol Gen Genet 1979 Jan 10
PMID:N-independent leftward transcription in coliphage lambda: deletions, insertions and new promoters bypassing termination functions. 28 66

We have isolated a plaque-forming derivative of phage Mu which carries a determinant for ApR. The biological properties of this MuAp phage are similar to those of normal Mu. Its genome contains a 1.1 kb substitution where Mu DNA from the right end of the G region has been replaced by a similar length of DNA from the transposon Tn3. This fragment of Tn3 DNA carries the ApR gene, but is no longer capable of independent transposition.
Mol Gen Genet 1979 May 04
PMID:The isolation and characterisation of a plaque-forming derivative of bacteriophage Mu carrying a fragment of Tn3 conferring ampicillin resistance. 28 92

The lymph node viable cells suspension of immunized mice was centrifugated. The supernatant was chromatographed in Sephadex G-200, and fractions were deproteinized. The deproteinized third fraction (Mol wt 30000) stimulated specifically the plaque-forming cells of intact mice immunized by SRBC. It restored the capacity to antibody production in the lethally irradiated intact mice protected by the syngeneic bone marrow. The activity of this fraction disappeared following treatment with RNA-ase, but not with DNA-ase or trypsin. The first and the second deproteinized fractions of the supernatant inhibited non-specifically the viable lymph node cells of the immunized animals in the intact mice immunized with SRBC.
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PMID:[Replacement of the helper function of T cells by an RNA-containing antigen-specific lysis factor]. 31 Dec 27

Derivatives of phage lambda are described for use as vectors for fragments of DNA generated with the HindIII and EcoRI restriction enzymes. With some vectors, hybrid molecules are recognised by a change from a turbid to a clear plaque morphology resulting from the insertion of a fragment of DNA into the lambda gene coding for the phage regressor. Other vectors contain a central, replaceable fragment of DNA which imparts a readily recognisable phenotype. This central fragment may include either a gene for a mutant transfer RNA (suppressor) or a part of the lacZ gene of E. coli able to complement a lacZ host. The appropriate lacZ host and indicator plates permit the ready distinction between recombinant and vector phages by the colour of the plaques.
Mol Gen Genet 1977 Jan 07
PMID:Lambdoid phages that simplify the recovery of in vitro recombinants. 31 44

A bacterial mutation affecting lambda DNA replication, called groPC756, has been mapped between the thr and leu bacterial loci. Most of the parental lambda DNA does not undergo even one round of replication in this host. Lambda mutants, call pi, which map in the lambda P gene are able to overcome the inhibitory effect of the groPC756 mutation. It is shown that the mutation at the groPC locus also interferes with bacterial growth at 42 degree C. A lambda-transducing phage,carrying the groPC+ allele, was isolated as a plaque-former on groPC756 bacteria. Upon lysogenization, it restores both the gro+ and temperature resistant phenotypes.
Mol Gen Genet 1977 Feb 28
PMID:A new bacterial gene (groPC) which affects lambda DNA replication. 32 65

Mutagenesis by 5-bromouracil of lambda phage to clear plaque formers does not depend on the recA function of the host E. coli cell or on the red function of the phage. Pretreatment of the host cells with ultraviolet light does not affect bromouracil mutagenesis of the adsorbed phage. Mutagenesis by hydroxlamine to clear plaque formers takes place at a high level in recA- host cells, and is not changed by preirradiation of of rec+ (wild type) hosts with ultraviolet light. Thus, bromouracil and hydroxylamine appear to mutate lambda phage by a process which differs from that responsible for ultraviolet mutagenesis. Two characteristics of bromouracil mutagenesis--the nonlinear dependence of the number of mutants on bromouracil incorporation, and a high frequency of heterozygotes--fit in with Rydberg's (1977) picture of bromouracil mutagenesis as a consequence of base mispairing, with mismatch repair removing the mutations at low incorporation of the analog.
Mol Gen Genet 1977 Mar 28
PMID:Mutagenesis of lambda phage: 5-bromouracil and hydroxylamine. 32 84

By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of temperature-resistant derivatives of the lysogen resulted in a fusion of the lac genes to a region of deoxyribonucleic acid that is transcribed under the control of the ilvC regulatory elements. A strain bearing the fusion was shown to be inducible for beta-galactosidase by acetohydroxybutyrate, a natural inducer of acetohydroxy acid isomeroreductase. Induction of the lysogen by mitomycin C led to the isolation of a plaque-forming lambda derivative carrying this ilvC-lac fusion.
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PMID:Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions. 33 10

A new approach for isolation of a plaque forming lambda specialized transducing phage is described. It consists of directed transposition of an F' plasmid into the gal region of a dnaAts galE- Escherichia coli strain by integrative suppression and deletion of the chlD region in order to shorten the distance between the marker of interest on the F' and the prophage serving to prepare an LFT1 lysate. An F' danC+thr+ plasmid was used here and lambdadthr and lambdaddnaC phages were isolated. In addition, lambdapdnaC was obtained from a double lysogen for lambdaddnaC and lambda b2.
Mol Gen Genet 1977 Oct 20
PMID:Directed integration of an F' plasmid by integrative suppression: isolation of plaque forming lambda transducing phage for the dnaC gene. 33 25


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