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Query: UNIPROT:P06889 (Mol)
 630,302 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It is shown that the formation of complexes with several arginine peptides stabilizes the B-form of DNA with 10 (+/- 0.15) base-pairs per turn at all relative humidities, even upon complete dehydration. From an analysis of the packing arrangement and from the calculated diffraction patterns, it is concluded that arginine is associated with DNA in its major groove. It is also shown that the diffraction pattern of nucleoprotamine can be interpreted by placing the protamine on the major groove of DNA. The strong intensity on the first layer-line is due to the influence of neutral residues on the diffraction pattern. Thus, we conclude that protamine is bound to the major groove of DNA.
J Mol Biol 1983 Jun 15
PMID:X-ray diffraction study of DNA complexes with arginine peptides and their relation to nucleoprotamine structure. 686 99

Phospholipid vesicles (liposomes) were subjected to dehydration-hydration cycles in the presence of 6-carboxyfluorescein or salmon sperm DNA. We found that the vesicles fused into multilamellar structures during dehydration with solutes trapped between the lamellae. Upon rehydration the lamellae swelled and formed large vesicular structures containing solute. This model can be used to study encapsulation of macromolecules by lipid membranes to form protocellular structures under prebiotic conditions.
J Mol Evol 1982
PMID:Encapsulation of macromolecules by lipid vesicles under simulated prebiotic conditions. 709 80

Using laser resonance Raman spectroscopy the influence of water on the structure of the chromophore centre in bacteriorhodopsin from Halobacterium halobium has been studied. The absorption band has been found to shift from 568 nm to 506 nm due to local protein changes in the chromophore centre near Schiff base bounding retinal with the lysine residue. These changes are not accompanied by the Schiff base deprotonation. Dehydration decreases essentially the reaction rate of the cis in equilibrium trans isomerization processes. In the dry state the potential barriers of the cis in equilibrium trans transition reaction turns out to be higher than that of the reverse reaction. As a result the equilibrium shifts to the cis-retinal form. Comparison of the Raman spectra of the M412 intermediate in wet and dry states of purple membranes leads to the conclusion that in water suspensions of purple membranes the chromophore state of the M412 intermediate is closer to cis- than to trans-retinal.
Mol Biol (Mosk)
PMID:[Investigation of the effects of dehydration on bacterial rhodopsin by laser resonance Raman spectroscopy]. 712 64

We have isolated a 7 kb EcoRI genomic fragment from Arabidopsis thaliana which contains, in a tandem arrangement, two closely related dhn/lea/rab-like genes, lti29 (formerly named lti45) and cor47, corresponding to previously isolated cDNA clones. Both transcripts have been shown to accumulate in response to low temperature (LT), abscisic acid (ABA) and dehydration. Alignment of the amino acid sequences of the deduced polypeptides showed that they are 67% identical. The calculated molecular masses of the two polypeptides were 29 kDa for LTI29 and 30 kDa for COR47. Both polypeptides contain one conserved serine-stretch and three lysine-rich repeats characteristic of DHN/LEA/RAB-like proteins. In addition, both LTI29 and COR47 harbour and N-terminal acidic repeat only found in a few members amongst the DHN/LEA/RAB proteins. The close distance between the two genes (separated by 2.7 kb) and their tandem organization in the A. thaliana genome as well as the overall homology at the nucleotide sequence level of the coding region suggest that the two genes have evolved through a duplication event. This seems to be a common feature among A. thaliana LT-responsive genes.
Plant Mol Biol 1995 Oct
PMID:Structure and organization of two closely related low-temperature-induced dhn/lea/rab-like genes in Arabidopsis thaliana L. Heynh. 757 89

The Arabidopsis thaliana genes kin1 and cor6.6 belong to the same family and were expressed at higher levels following low temperature and ABA treatments. In an attempt to elucidate the mechanism of gene regulation by low temperature, the relationship between low-temperature- and abscisic acid (ABA)-induced gene expression and possible differential expression of the two genes, we have cloned a 5.3 kb genomic fragment harboring kin1 and cor6.6 and their respective 5' sequences. The putative promoters of both genes were fused to the beta-glucuronidase (GUS) coding sequence and GUS expression was analysed in transgenic tobacco and Arabidopsis plants. The cor6.6 promoter produced a higher basal level of expression than the kin1 promoter in transgenic tobacco. Enzyme assays of inducible GUS activity in transgenic Arabidopsis and tobacco plants showed that GUS activity directed by both kin1 and cor6.6 promoters was significantly induced by ABA, dehydration and osmoticum, but not by low temperature. Northern analysis revealed, in contrast, that GUS mRNA was significantly induced in these transgenic plants by low temperature. Further analysis showed that, at low temperature, GUS protein synthesis from the induced GUS mRNA was inhibited. Together these results reveal induction of kin1 and cor6.6 transcription by low temperature, exogenous ABA and dehydration. However, low-temperature expression is dramatically reduced at the translational level.
Plant Mol Biol 1995 Jul
PMID:Promoters from kin1 and cor6.6, two homologous Arabidopsis thaliana genes: transcriptional regulation and gene expression induced by low temperature, ABA, osmoticum and dehydration. 764 94

Changes in RNA of Baker's yeast have been studied during a thermal dehydration, through which the cellular moisture decreased from 70% to 9%, followed by a mortality of cells being less than 8%, and by consumption of the cellular glycogen. Simultaneously, the RNA of 4.2s and 5.8s increase, however, the RNA of 12.0s and 18.0s decrease during the thermal cellular dehydration. The contents of the low molecular mass RNA may not be affected by incubation of chloromycetin and penicillin in media during the fed-batch culture. It suggests that RNA play a part during the cellular thermal dehydration.
Biochem Mol Biol Int 1995 May
PMID:Changes in RNA of Saccharomyces cerevisiae during thermal cellular dehydration. 766 3

Diffusion of the fluorescent membrane probe, Dil-C16 (3), from labelled to unlabelled human erythrocytes has been employed to monitor hemi-fusion (membrane fusion) in monolayers of cells exposed to poly(ethylene glycol) (PEG). Diffusion of the cytoplasmic probe, 6-carboxyfluorescein, was used similarly to monitor cell fusion (cytoplasmic mixing). Hemi-fusion, which is normally seen when erythrocytes are exposed to dehydrating concentrations of commercial PEG 6000, did not occur when the PEG was pretreated with Chelex 100 resin to remove metal ions. Cytoplasmic mixing, which is normally observed when the dehydrated erythrocytes are substantially rehydrated, also failed to occur when both PEG 6000 and the rehydrating buffer had been treated with Chelex 100. The re-addition to Chelex-treated PEG of components removed by the resin, and the addition of 10 mu mM concentrations of La3+ or Al3+, restored its ability to induce hemi-fusion and cell fusion. Higher concentrations of several other metals, including Ca2+, were also effective. These observations show that metal ions are required for hemi-fusion with erythrocytes in the presence of PEG, and that dehydration alone is insufficient to induce hemi-fusion. Phosphatidylserine was apparently not accessible in erythrocytes treated with PEG 6000 until the cells were rehydrated. This indicates that metal ions do not assist the hemi-fusion of erythrocytes by forming trans complexes with surface phosphatidylserine when the cells are dehydrated by PEG.(ABSTRACT TRUNCATED AT 250 WORDS)
Mol Membr Biol
PMID:Interactions between metal ions and poly(ethylene glycol) in the fusion of human erythrocytes. 774 82

In Arabidopsis thaliana, the induction of a dehydration-responsive gene, rd22, is mediated by abscisic acid (ABA) but the gene does not include any sequence corresponding to the consensus ABA-responsive element (ABRE), RYACGTGGYR, in its promoter region. The cis-regulatory region of the rd22 promoter was identified by monitoring the expression of beta-glucuronidase (GUS) activity in leaves of transgenic tobacco plants transformed with chimeric gene fusions constructed between 5'-deleted promoters of rd22 and the coding region of the GUS reporter gene. A 67-bp nucleotide fragment corresponding to positions -207 to -141 of the rd22 promoter conferred responsiveness to dehydration and ABA on a non-responsive promoter. The 67-bp fragment contains the sequences of the recognition sites for some transcription factors, such as MYC, MYB, and GT-1. The fact that accumulation of rd22 mRNA requires protein synthesis raises the possibility that the expression of rd22 might be regulated by one of these trans-acting protein factors whose de novo synthesis is induced by dehydration or ABA. Although the structure of the RD22 protein is very similar to that of a non-storage seed protein, USP, of Vicia faba, the expression of the GUS gene driven by the rd22 promoter in non-stressed transgenic Arabidopsis plants was found mainly in flowers and bolted stems rather than in seeds.
Mol Gen Genet 1995 May 20
PMID:Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis. 777 45

A need for the computer simulation of hydration/dehydration processes in functional aluminosilicate structures has been noted. Full and realistic simulations of these systems can be somewhat ambitious and require the aid of interactive computer graphics to identify key structural/chemical units, both in the devising of suitable water-ion simulation potentials and in the analysis of hydrogen-bonding schemes in the subsequent simulation studies. In this article, the former is demonstrated by the assembling of a range of essential water-ion potentials. These span the range of formal charges from +4e to -2e, and are evaluated in the context of three types of structure: a porous zeolite, calcium silicate cement, and layered clay. As an example of the latter, the computer graphics output from Monte Carlo computer simulation studies of hydration/dehydration in calcium-zeolite A is presented.
J Mol Graph 1995 Feb
PMID:Modeling cation/anion-water interactions in functional aluminosilicate structures. 779 32

Exposure of yeast cells to high osmolarities leads to dehydration, collapse of ion gradients over the plasma membrane and decrease in cell viability. The response of yeast cells to high external osmolarities is designated osmostress response. It is likely that both osmoregulatory and general stress reactions are involved in this so far poorly understood process. Part of the response aims at raising the internal osmotic potential, i.e. the production of osmolytes such as glycerol, and exclusion of toxic solutes. In addition, heat-shock proteins and trehalose are synthesized, probably to protect cellular components and to facilitate repair and recovery. Recent analyses of osmosensitive yeast mutants strongly suggest the involvement of protein kinase-mediated signal-transduction pathways in the maintenance of the osmotic integrity of the cell. This has stimulated interesting hypotheses as to the actual osmosensing mechanism.
Mol Microbiol 1993 Oct
PMID:Osmostress response of the yeast Saccharomyces. 793 16


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