Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P06126 (CD1a)
 2,221 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have attempted to improve retrovirus-mediated gene transfer efficacy into hematopoietic progenitor cells (HPCs) without causing them to lose their lymphoid potential. Highly purified CD34(+) cells on CH-296 fibronectin fragments have been transduced with three different cytokine combinations. Murine CD2 was used as a marker gene. Transgene expression was assayed by FACS analysis shortly after transduction of CD34(+) cells and after long-term culture (LTC) extended by differentiation of various lymphoid lineages: NK cells, B cells, and dendritic cells. Compared with the historical cytokine mix, i.e., SCF (stem cell factor) + IL-3 (interleukin 3) + IL-6, the combination SCF + FL (Flt-3 ligand) + M-GDF (megakaryocyte growth and differentiation factor) + IL-3 significantly improved the total number of viable cells and CD34(+) cells after transduction and the long term-cultured progenitors after 6 weeks. In addition, the combination of SCF + FL + M-GDF + IL-3 maintained more efficiently the lymphoid potential of the progeny of transduced long term-cultured CD34(+) cells, as attested by the significantly higher number of CD56(+), CD19(+), and CD1a(+) cells recovered when FL and M-GDF were added to SCF + IL-3. Thus, even though additional improvements may still be needed in transduction of HPCs, these conditions were adopted for a clinical trial of gene therapy for X-linked severe combined immunodeficiency.
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PMID:Optimization of retroviral gene transfer protocol to maintain the lymphoid potential of progenitor cells. 1117 65

To study the feasibility of in a A patient with extramedullary hematopoiesis presenting as a posterior mediastinal tumor underwent fine-needle aspiration for cell pathology diagnosis. The primary locus of a posterior mediastinal extramedullary hematopoiesis was examined with Papanicolaou staining and HE staining, and the expressions of cytokeratin, epithelial membrane antigen (EMA), terminal deoxynucleotidyl transferase, CD3, CD20, anaplastic lymphoma kinase, CD34, CD235a, myeloperoxidase, CD61, P53, CD30, S-100, CD1a, and Ki-67 with immunohistochemistry. The results were analyzed of bone marrow biopsy and cell smears, examination of chromosome structure and number, and detection of BCR/ABL fusion gene using fluorescence in situ hybridization. Examination of cell pathology of fine-needle aspiration in the posterior mediastinal focus revealed scatter cells of heterogeneous sizes consisting mainly of erythroid cells, and granulocytes and erythroid cells at different stages and lobulated large mature megakaryocytes were found. The eythroid cells in the core biopsy tissue were distributed in multiple cell islands. Immunohistochemistry showed positive results for erythroid cell CD235a, granulocyte MPO, megakaryocyte CD61, and Ki-67 (about 90%). Examination of the bone barrow biopsy tissue and cell smear also showed the changes of hyperplastic anemia without clone structure or abnormal number of the chromosomes, and no BCR/ABL fusion gene was detected by fluorescence in situ hybridization. Fine-needle aspiration cell pathology combined with the patient's clinical data allows the diagnosis of extramedullary hematopoiesis in the rare site of the posterior mediastinum.
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PMID:[Fine-needle aspiration cell pathology for diagnosis of intrathoracic extramedullary hematopoiesis presenting as a posterior mediastinal tumor: a case report]. 2853 98