Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P06126 (CD1a)
 2,221 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A panel of 380 commercially available monoclonal antibodies (mAbs) against human CD molecules from various sources was tested during the 8th Human Leukocyte Differentiation Antigen Workshop (HLDA8) for cross-reactivity on canine peripheral blood leukocytes by flow cytometry. In addition, all mAbs were used to label a 50:50 mixture of platelets and erythrocytes of the same dogs. This testing resulted in 51 cross-reacting mAbs. mAbs with specificity for CD9, CD29, CD42a, CD61, and CD41/CD61 showed cross-reactivity with canine platelets in a non-polymorphic and one mAb with the erythrocyte antigen CD235a in a polymorphic reaction pattern. Canine leukocyte-reactive mAbs included those with specificity for CD11a, CD11b, CD14, CD18, CD21, CD22, CD47, CD49d, CD49e, CD56, CD62L, CD91, CD94, and CD172a. In addition, several mAbs resulted in a staining pattern of canine cells which suggest that the canine epitope equivalents have an alternate expression pattern from that expected for humans (CD1a, CD35, CD44, CD45, CD75s, CD81). In summary, this study confirmed the reactivity of previously described cross-reactive mAbs with canine cells and resulted in the characterization of mAbs recognizing so far undetectable canine CD molecules.
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PMID:Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes. 1764 96

To study the feasibility of in a A patient with extramedullary hematopoiesis presenting as a posterior mediastinal tumor underwent fine-needle aspiration for cell pathology diagnosis. The primary locus of a posterior mediastinal extramedullary hematopoiesis was examined with Papanicolaou staining and HE staining, and the expressions of cytokeratin, epithelial membrane antigen (EMA), terminal deoxynucleotidyl transferase, CD3, CD20, anaplastic lymphoma kinase, CD34, CD235a, myeloperoxidase, CD61, P53, CD30, S-100, CD1a, and Ki-67 with immunohistochemistry. The results were analyzed of bone marrow biopsy and cell smears, examination of chromosome structure and number, and detection of BCR/ABL fusion gene using fluorescence in situ hybridization. Examination of cell pathology of fine-needle aspiration in the posterior mediastinal focus revealed scatter cells of heterogeneous sizes consisting mainly of erythroid cells, and granulocytes and erythroid cells at different stages and lobulated large mature megakaryocytes were found. The eythroid cells in the core biopsy tissue were distributed in multiple cell islands. Immunohistochemistry showed positive results for erythroid cell CD235a, granulocyte MPO, megakaryocyte CD61, and Ki-67 (about 90%). Examination of the bone barrow biopsy tissue and cell smear also showed the changes of hyperplastic anemia without clone structure or abnormal number of the chromosomes, and no BCR/ABL fusion gene was detected by fluorescence in situ hybridization. Fine-needle aspiration cell pathology combined with the patient's clinical data allows the diagnosis of extramedullary hematopoiesis in the rare site of the posterior mediastinum.
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PMID:[Fine-needle aspiration cell pathology for diagnosis of intrathoracic extramedullary hematopoiesis presenting as a posterior mediastinal tumor: a case report]. 2853 98