Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P05231 (
interleukin-6
)
23,907
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The human urea transporter
SLC14A1
(HUT11/UT-B) has been suggested as a marker for the adipogenic differentiation of bone cells with a relevance for bone diseases. We investigated the function of
SLC14A1
in different cells models from bone environment.
SLC14A1
expression and cytokine production was investigated in bone cells obtained from patients with osteoporosis. Gene and protein expression of
SLC14A1
was studied during adipogenic or osteogenic differentiation of human mesenchymal progenitor cells (hMSCs) and of the single-cell-derived hMSC line (SCP-1), as well as in osteoclasts and chondrocytes. Localization was determined by histochemical methods and functionality by urea transport experiments. Expression of
SLC14A1
mRNA was lower in cells from patients with osteoporosis that produced high levels of cytokines. Accordingly, when adding a combination of cytokines to SCP-1
SLC14A1
mRNA expression decreased.
SLC14A1
mRNA expression decreased after both osteogenic and more pronounced adipogenic stimulation of hMSCs and SCP-1 cells. The highest
SLC14A1
expression was determined in undifferentiated cells, lowest in chondrocytes and osteoclasts. Downregulation of
SLC14A1
by siRNA resulted in an increased expression of
interleukin-6
and interleukin-1 beta as well as adipogenic markers. Urea influx through
SLC14A1
increased expression of osteogenic markers, adipogenic markers were suppressed.
SLC14A1
protein was localized in the cell membrane and the cytoplasm. Summarizing, the
SLC14A1
urea transporter affects early differentiation of hMSCs by diminishing osteogenesis or by favoring adipogenesis, depending on its expression level. Therefore,
SLC14A1
is not unequivocally an adipogenic marker in bone. Our findings suggest an involvement of
SLC14A1
in bone metabolism and inflammatory processes and disease-dependent influences on its expression.
...
PMID:Decreased Expression of the Human Urea Transporter SLC14A1 in Bone is Induced by Cytokines and Stimulates Adipogenesis of Mesenchymal Progenitor Cells. 3195 45
