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Target Concepts:
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Query: UNIPROT:P05231 (
interleukin-6
)
23,907
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Interleukin-6
(
IL-6
), a cytokine produced by skeletal cells, increases bone resorption, but its effects on collagenase expression are unknown. We tested the effects of
IL-6
and its soluble receptor on
collagenase 3
expression in osteoblast-enriched cells from fetal rat calvariae (Ob cells).
IL-6
caused a small increase in collagenase mRNA levels, but in the presence of
IL-6
-soluble receptor (IL-6sR),
IL-6
caused a marked increase in collagenase transcripts after 2-24 h. In addition, IL-6sR increased collagenase mRNA when tested alone.
IL-6
and IL-6sR increased immunoreactive collagenase levels. Cycloheximide and indomethacin did not prevent the effect of
IL-6
and IL-6sR on collagenase mRNA levels.
IL-6
and IL-6sR did not alter the decay of collagenase mRNA in transcriptionally arrested Ob cells and increased the levels of collagenase heterogeneous nuclear RNA and the rate of collagenase gene transcription in Ob cells.
IL-6
and IL-6sR increased
collagenase 3
mRNA in MC3T3 cells but only modestly in skin fibroblasts.
IL-6
and IL-6sR enhanced the expression of tissue inhibitor of metalloproteinases 1. In conclusion,
IL-6
, in the presence of IL-6sR, increases
collagenase 3
synthesis in osteoblasts by transcriptional mechanisms. This effect may contribute to the action of
IL-6
on bone matrix degradation and bone resorption.
...
PMID:Interleukin-6 and its soluble receptor cause a marked induction of collagenase 3 expression in rat osteoblast cultures. 911 85
The response of human T lymphocytes to various stimuli includes the expression of the matrix metalloproteinase (MMP) genes stromelysin 2, gelatinase A and gelatinase B. The proteins encoded by these genes could confer the capacity to degrade macromolecular components of the extracellular matrix (ECM), and to shed transmembrane proteins such as tumor necrosis factor (TNF), TNF receptor,
Interleukin-6
receptor and Fas ligand. To identify further MMP genes transcribed in T lymphocytes exposed to phorbol 12-myristate 13-acetate and a calcium ionophore, we combined reverse transcription and polymerase chain reaction using primers specific for conserved domains and detected
collagenase 3
transcripts, first described in a human breast cancer. However, when the sequence of the complementary DNA was compared, additional 23 nucleotides were found in the 5' nontranslated region of the lymphocyte messenger RNA (mRNA). Northern blot analysis revealed 2 major inducible mRNA species of 1.9 and 2.8 kilobases, whose levels were lower than those of stromelysin 2. The observation that activated T lymphocytes transcribe several MMP genes, including a collagenase, indicates that the effector functions of these cells include enzymatic activities towards most constituents of the ECM, as well as some transmembrane proteins relevant to inflammation and apoptosis.
...
PMID:A matrix metalloproteinase gene expressed in human T lymphocytes is identical with collagenase 3 from breast carcinomas. 956 63
