Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P05231 (
interleukin-6
)
23,907
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have previously shown that L58 in the putative 5th helical region of human
interleukin-6
(
IL-6
) is important for activation of the
IL-6
signal transducer gp130 [de Hon et al. (1995) FEBS Lett. 369, 187-191]. To further explore the importance of individual residues in this region for gp130 activation we have now combined Ala substitutions of residues E52,
S53
, S54, K55, E56, L58 and E60 with other substitutions in
IL-6
, known to affect gp130 activation (Q160E and T163P). The combination mutant protein with L58A completely lost the capacity to induce the proliferation of XG-1 myeloma cells, and could effectively antagonize wild type
IL-6
activity on these cells. Moreover, the data suggest that besides L58, S54 particularly, but also E52,
S53
, K55 and E56 contribute to gp130 activation.
...
PMID:Identification of residues in the putative 5th helical region of human interleukin-6, important for activation of the IL-6 signal transducer, gp130. 889 3
