Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: UNIPROT:P05231 (
interleukin-6
)
23,907
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cystatins are physiological cysteine proteinase inhibitors. Here we report a novel function for some family 2 cystatins that is not related to these activities. The release of
interleukin-6
(
IL-6
) and interleukin-8 (IL-8) by the gingival fibroblasts and that of
IL-6
by murine splenocytes were measured using ELISA systems specific for these cytokine molecules. Family 2 cystatins, including cystatins C, SA1,
SA2
, S, and egg white cystatin, upregulated the
IL-6
production by two-lasts at physiological concentrations. After complete saturation with papain, those family 2 cystatins still upregulated
IL-6
production, suggesting that the papain-inhibitory site was not involved in the cytokine-inducing activity.
...
PMID:Cytokine-inducing activity of family 2 cystatins. 1115 74
Recently, family 2 cystatins have been demonstrated to upregulate
interleukin-6
(
IL-6
) production by human gingival fibroblasts [Biol. Chem. 381 (2000) 1143]. To elucidate the mechanism of the
IL-6
inducing activity of cystatins, we tested NF-kappa B activation with salivary cystatins SA1 and
SA2
-stimulated human gingival fibroblast whole cell lysates. The
IL-6
production by human fibroblasts in response to these cystatins was inhibited by tyrosine kinase inhibitors and an inhibitor of NF-kappa B activation. The
IL-6
inducing activity of the cystatins was depressed by the anti-CD58 monoclonal antibody. These findings supply evidence that cystatins SA1 and
SA2
adhere to human fibroblasts and that the event results in tyrosine phosphorylation and upregulation of the release of
IL-6
mediated enhancement of NF-kappa B activity.
...
PMID:Salivary cystatins induce interleukin-6 expression via cell surface molecules in human gingival fibroblasts. 1241 93
Recently, it has been demonstrated that family 2 cystatins upregulate
interleukin-6
production by human gingival fibroblasts. In the present study, we investigated the effects of cystatin SA on cytokine production by helper T cells. Human CD4-positive T cells were cultured with phytohemagglutinin in the presence or absence of 0.1 microM recombinant cystatin SA1 or
SA2
. When the amounts of interleukin-4 (IL-4) and interferon-gamma (IFNgamma) were analyzed in an ELISA system after stimulation with either cystatin, no significantly increased levels of IL-4 were detected. However, the amounts of IFNgamma were significantly increased after stimulation with the cystatins. Our results suggest that salivary family 2 cystatins are involved in immune responses through the cytokine network.
...
PMID:Cystatin SA, a cysteine proteinase inhibitor, induces interferon-gamma expression in CD4-positive T cells. 1519 2
