Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P05231 (
interleukin-6
)
23,907
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
PSGR
is a newly identified human prostate tissue-specific gene belonging to the G-protein coupled receptor (GPCR) family. Overexpression of
PSGR
is associated with human prostate intraepithelial neoplasia (PIN) and prostate tumors, suggesting
PSGR
may play an important role in early prostate cancer development and progression. To understand the regulation of tissue-specific expression of human
PSGR
and its upregulation mechanism in prostate cancers, we characterized the promoter region of
PSGR
and analyzed the control mechanism for
PSGR
expression in human prostate tissues/cells. In this report, we demonstrate that two distinct promoters control the transcriptional regulation of
PSGR
in human prostate cells. The first promoter region includes exon 1 and a TATA box at -31 site. The minimal DNA sequence with promoter activity is about 123 bp upstream of exon 1. Exon 1 contains tissue specific regulatory activity for the first promoter of
PSGR
gene. The second promoter is located in the upstream region of exon 2, which is a TATA-less and non-GC-rich promoter. Primer extension and RNA protection assays (RPA) revealed that the transcription driven by the second promoter is initiated at the junction of intron and exon 2 within a cluster of nucleotides located about 250 bp upstream from the junction. Both promoters show prostate cell-specific characteristics in our luciferase assays in transfected cells. Furthermore, we investigated the regulation of the promoter activities of the
PSGR
gene by different growth factors and cytokines, and demonstrated that
interleukin-6
(
IL-6
) activates the promoter activities of
PSGR
in human prostate cancer cells. These data suggest that two functional promoters regulate the transcriptional expression of
PSGR
in human prostate tissues and
PSGR
is a new target for
IL-6
transcriptional regulation.
...
PMID:Regulation of human prostate-specific G-protein coupled receptor, PSGR, by two distinct promoters and growth factors. 1614 59
