Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P04637 (p53)
 77,613 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The alterations of multiple genes and their carcinogenic mechanism in cervical carcinoma were studies by molecular hybridisation, PCR and PCR-ASO techniques. The G-T point mutation in the 12th coden of Ha-ras was detected in cervical carcinomas with mutation frequency of 18.2% (8/44), and the amplification rate of Ha-ras gene was 45% (9/20). The c-erb B2 was amplified 3-30 fold with an amplification rate of 73.3% (11/15) in cervical carcinomas and 5 cancerous samples showed gene rearrangement. The elevated copies of c-myc gene with amplification rate of 91.7% (11/12) were observed in cervical carcinomas. The study of HPV16 viral gene showed that the existence of HPV16 DNA sequence was positively associated with c-myc gene amplification in cervical cancerous samples. The p53 and Rb tumor suppressor genes absence of deletion were observed in the 12 specimens of cervical carcinoma investigated. As mentioned above, the study on alteration and carcinogenic mechanism of multiple genes indicated that 3 oncogenes and HPV16 viral gene were activated or integrated throygh different mechanisms and they played roles in co-carcinogenesis. The integration of HPV16 gene might promote the c-myc gene at the early stage in carcinogenesis of cervical carcinoma, while the alteration of Ha-ras and c-erb B2 gene might be middle-late event. As for the roles of the p53 and Rb tumor suppresor gene in cervical carcinogenesis need further researches.
Hua Xi Yi Ke Da Xue Xue Bao 1996 Mar
PMID:[Carcinogenic mechanisms of multiple genes in cervical carcinoma]. 920 11

In order to increase the sensitivity to immunostaining in formalinfixed, paraffin-embedded tissues, we developed an immunohistochemical method by microwave heating of tissue sections instead of trypsin digestion. The results of this study showed that there was no positive P53 protein reaction in normal and hyperplastic mucousa of human mouth, whereas 90% (27/30) of cases with dysplasia, 61% (30/49) of oral squamous cell carcinomas and 86% (13/15) of regional metastatic lymph nodes were positive. And all positive reactions were localized in nuclei. Comparison of these positive results of p53 gene mutation detected by silver staining method with the results by polymerase chain reaction-single strand conformation polymorphism analysis did not reveal matched results, especially during the precancerous period. The authors analysed the causes of difference not only by methodology, but also by cell groups which had different genetic changes in tissues of precancerous lesions.
Hua Xi Yi Ke Da Xue Xue Bao 1996 Sep
PMID:[The role of p53 gene during the development of human oral malignant lesions: a comparative study of p53 gene mutation with P53 protein positive immunostaining]. 938 53

This study sought to address the relationship between crocidolite and p53 gene mutation. The mutations of p53 gene in 8 BALB/c 3T3 cell lines transformed by crocidolite were analysed. Altogether 11 exons of the gene from 8 cell lines were detected by PCR-SSCP. 7 alterations were found; 2 of them were located in exon 4-6, and 5 in 9-11. Most of the mutations (5/7) were of one more band than that of wild cell from SSCP, and alterations were randomly scattered among the crocidolite doses groups. The results suggest that the presence of a p53 alteration is not related to the dose of crocidolite used. Besides, p53 mutation may occur in a relatively later period of the growth of the transformed cell lines. The results also showed that the mutations occurred predominantly in exons 9-11. This was different from that seen in human mesothelioma where mutations in the exon 5-8 of p53 gene were more frequently observed.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Dec
PMID:[p53 gene mutations in BALB/c 3T3 cells transformed by crocidolite]. 1068 50

An immunohistochemical method utilizing avidin-biontin complex (ABC) technique was used in this study to detect P16 and P53 protein expressions in 40 ovarian serous cystadenocarcinomas and 10 serous cystadenomas. The results showed that the total positive rates of P16 and P53 protein were 40% and 60% respectively. The positive staining rate was higher in P53 protein expression than in P16 protein expression in the same cases. The positive staining rates of P16 protein were 16.67%, 33.33% and 52.63% respectively in stages I, II and III. There was no significant difference in histological grading. The postive staining rate of P53 protein expression in poorly differentiated tumor was higher than that in well differentiated group (P < 0.05). These results suggested that P16 and P53 protein expressions of the ovarian serous cystadenocarcinoma might be correlated with human organs, histological type of tumor, cyclin and cyclindependent kinase.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Dec
PMID:[A study on P16 and P53 protein expressions in ovarian serous cystadenocarcinoma]. 1068 58

To study the relationship of P53 protein expression with clinical course and prognosis in malignant germ cell tumor of ovary P53 protein expression was examined by immunohistochemical ABC methods in 82 cases of the neoplasm and 20 cases of normal ovary tissue. The results showed that no expression of P53 protein was detected in normal ovarian tissue. The total expression rate of P53 protein was 24.39% in neoplasm, and the expression was not significantly correlated with the different histological types of neoplasm. The study however found that the protein expression of p53 gene was significantly correlated with the clinical stages and prognosis of the neoplasm.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Dec
PMID:[P53 protein expression in malignant germ cell tumor of ovary and its relationship with clinical course and prognosis]. 1068 59

In order to investigate the prognostic significance of P53 protein expression in the patients with colorectal adenocarcinoma, we used the P53 monoclonal antibody and ABC immunohistochemical method combined with the repairing method for antigen by microwave oven to study the relationship between the overexpression of P53 protein and the prognosis in 40 cases of colorectal adenocarcinoma. The results showed that 65% (26/40) cases of colorectal adenocarcinoma were positive for overexpression of P53 protein. The 5-year survival rate for positive cases was 50%, but 87.5% for negative cases. These findings suggest that the overexpression of P53 protein closely associates with the prognosis in colorectal adenocarcinoma (P < 0.05), thus it might become a new prognostic indicator. The prognosis in colorectal adenocarcinoma could be predicted thoroughly by considering the expression of P53 protein in combination with the traditional prognostic factors.
Hua Xi Yi Ke Da Xue Xue Bao 1998 Jun
PMID:[Prognostic significance of P53 protein expression in patients with colorectal adenocarcinoma]. 1068 11

In order to observe the relation of tumor supperssor gene p53 and Rb gene to nasopharyngeal carcinoma, we investigated p53 gene mutations in exon 7-8 in 33 cases of nasopharygeal carcinoma (NPC), using single-strand conformation ploymorphism analysis of PCR (PCR-SSCP). The data showed p53 mutations in 7 of 33 specimens at exon 8 (21.2%). No mutation in exon 7 was detected. Analysis of Rb gene in NPC tissues by southern blot revealed the deletion and loss of activity of Rb gene in 11 of 13 cases (86.6%). Our results suggest that Rb gene and p53 gene are closely associated with the tumorigenesis of NPC.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Sep
PMID:[The expression of tumor suppressor gene p53 and Rb gene in nasopharyngeal carcinoma]. 1068 31

In this study PCR-SSCP alalysis was made to detect the mutation of the exon 5-7 of p53 gene in 37 cases of human primary brain tumor. The results showed that 10 human primary brain tumors had mutation, of which 9 were gliomas (37.5%) and 1 was meningioma (7.7%), and that the higher the grade of tumor was, the higher would be the frequency of mutation. These results suggest that human primary brain tumor is related to p53 gene mutation and such mutation may be the major reason for human primary brain tumor genesis and malignancy.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Mar
PMID:[PCR-SSCP analysis of p53 gene in human primary brain tumor]. 1068 61

This study screened 32 cases of hepatocellular carcinoma (HCC) from Chengdu detecting HBV DNA. HBsAg and p53 mutations by using Southern blot hybridization, immunohistochemistry and polymerase chain reaction/restriction enzyme digest methods, respectively. The results revealed all the cases had been infected by HBV; the frequency of HBV DNA integration into HCC cell was 72%, and the positive staining for HBsAg 96.3%(26/27). Of the 32 cases, 8 showed nuclear staining of p53 protein (25%), no mutation of p53 gene at all in 27 nontumorours liver tissues was identified. The resluts suggest the inactivation of p53 function may play a significant role in the genesis of HBV-associated HCC; however, the largely negative p53 mutation results in the current study conversely indicate that hepatocarcinogenesis may even involve other comprehensive mechanisms. Further studies are worth doing to evaluate the possible contribution of HBV to the p53 mutation in HCC.
Hua Xi Yi Ke Da Xue Xue Bao 1997 Mar
PMID:[Detection of p53 gene mutations in hepatocellular carcinoma]. 1068 62

To investigate the relationship between immunoreaction of histologic subtype and prognosis, this paper analysed the clinicopathological data from 20 cases of hepatoblastoma. Immunohistochemical staining was performed in 18 cases. The results showed that cytopolasmic postivities of epithlial tumor cells were observed by CK, AFP, S-100 protein and vimentin in 14, 10, 9 and 4 cases respectively. Positive staining for CEA was seen in the nuclei of epithelial tumor cells in 11 cases. Nuclear P53 protein staining was found in 9 cases. Nuclear and cytoplasmic postivities of P16 protein were observed in 7 cases. S-100 protein, vimentin, CK and P16 protein were detected in mesenchymal component in 1 case. This study suggested that immunoreactions of hepatoblastomas were different in histologic subtypes. The expression may correlate with the neoplastic differentiation and prognosis.
Hua Xi Yi Ke Da Xue Xue Bao 1998 Sep
PMID:[Clinicopathological and immunohistochemical study of hepatoblastoma]. 1068 98


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