Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P04637 (
p53
)
77,613
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Our previous studies indicated that
SPATA12
, a novel spermatogenesis-associated gene, might be an inhibitor involved in spermatogenesis and tumorigenesis. To obtain a better understanding of the functions of
SPATA12
, a yeast two-hybrid screening system was used to search for interacting proteins, and chromodomain helicase DNA binding protein 2 (CHD2) was successfully identified. Bimolecular fluorescence complementation (BiFC) and subcellular co-localization assays further suggested a possible interaction between
SPATA12
and CHD2 in the nuclei. CHD2 is known to be involved in the later stage of the DNA damage response pathway by influencing the transcriptional activity of
p53
. Thus, our hypothesis is that
SPATA12
might play a role in DNA damage signaling. Western blotting results showed that
SPATA12
expression could be induced in ultraviolet-C (UV-C) irradiated cells. Through reporter gene assays and the activator protein-1 (AP-1) decoy oligodeoxynucleotide method, we demonstrated that
SPATA12
promoter activity could be up-regulated in response to UV-C radiation exposure and an AP-1 binding site in the
SPATA12
promoter may have a role in transcriptional regulation of
SPATA12
. Using colony formation and host cell reactivation assays, it was demonstrated that
SPATA12
might lead to inhibition of cellular proliferation in UV-C-irradiated DNA damage. Furthermore,
SPATA12
was transfected into H1299, MCF-7 and HeLa cells, and flow cytometry (FCM) results suggested that there are some biological association between
SPATA12
and
p53
in UV-C-irradiated DNA damage. In addition, we investigated whether
SPATA12
could up-regulate the expression of
p53
. Taken together, these findings indicate that
SPATA12
could be induced under UV-C stress. During DNA damage process, AP-1 involves in the transcriptional up-regulation of
SPATA12
in response to UV-C radiation and
p53
involves in growth inhibitory effects of
SPATA12
on UV-C irradiated cells.
...
PMID:SPATA12 and its possible role in DNA damage induced by ultraviolet-C. 2420 57
