Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P04637 (
p53
)
77,613
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
2-Amino-4,6-dinitrotoluene
(2-Am-DNT) and its isomers are the most common metabolites of 2,4,6-trinitrotoluene (TNT). It is desirable to know the toxicity of this metabolite particularly because of its role in carcinogenicity and because it could contaminate drinking water. We used MCF-7 human breast cancer cells which have
p53 tumor suppressor
gene in wild type form in both the loci. Immunoblotting with
p53
antibody showed enhanced
p53
level in treated cells compared to untreated control cells. Similarly,
p53
DNA-protein binding assays (gel-shift) showed accumulation of
p53 protein
in treated cells. This is the first report which shows
p53
accumulation in 2-Am-DNT treated cells providing evidence of potential carcinogenic effects of 2-Am-DNT.
...
PMID:Effects of 2-amino-4,6-dinitrotoluene on p53 tumor suppressor gene expression. 1457 14
