Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P04637 (
p53
)
77,613
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of 4-hydroxybenzyl isothiocyanate (HBITC), a natural H
2
S-donor from white mustard seeds (Sinapis alba), on the proliferation of human neuroblastoma (SH-SY5Y) and glioblastoma (U87MG) cells was studied and some aspects of the mechanism of its activity were suggested. The inhibition of both SH-SY5Y and U87MG cell proliferation was associated with an increase in the thiosulfate level, the number of cells with the inactive form of Bcl-2 protein, and with a decrease of mitochondrial membrane potential. Interestingly, HBITC results in downregulation of
p53 protein
and upregulation of p21 protein levels in SH-SY5Y cells. In the presence of elevated levels of H
2
S and thiosulfate, the sulfhydryl groups of
p53 protein
as well as Bcl-2 protein could be modified via HBITC-induced S-sulfuration or by oxidative stress. It seems that the induction of p21 protein level is mediated in SH-SY5Y cells by
p53
-independent mechanisms. In addition, HBITC-treatment caused downregulation of the level of mitochondrial
rhodanese
and 3-mercaptopyruvate sulfurtransferase, and consequently increased the level of the reactive oxygen species in SH-SY5Y cells.
...
PMID:New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H
2
S-donor. 2950 61
